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RNAseq
•
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0
votes
4
replies
1.2k
views
How to analyze 10 samples (5 control, 5 treatment) which perform the experiment from different day
DESeq2
RNASeq
4.0 years ago
Yosapol
• 0
0
votes
5
replies
2.4k
views
RNA-seq with patient samples without replicates with multiple timepoints
edgeR
RNASeq
multi
updated 4.0 years ago by
Gordon Smyth
53k • written 4.0 years ago by
kevin
• 0
0
votes
2
replies
1.3k
views
How to convert TMM RNA-seq file (generated by edgeR) to list of differentially expressed genes
edgeR
DifferentialExpression
TMM
RNASeq
updated 4.0 years ago by
Gordon Smyth
53k • written 4.0 years ago by
hamidrezarazzaghian
• 0
0
votes
2
replies
1.4k
views
How similar or disimilar are two sets of DEG?
DESeq2
RNASeq
updated 4.0 years ago by
Michael Love
43k • written 4.0 years ago by
curiousmind007
▴ 10
0
votes
2
replies
1.9k
views
voom flips the sign relative to log(cpm)
limma
limma-voom
rnaseq
voom
updated 4.1 years ago by
Gordon Smyth
53k • written 4.1 years ago by
taur.vil
• 0
0
votes
1
reply
2.4k
views
Gene expression analysis between three different tissues
DESeq2
RNAseq
updated 4.1 years ago by
James W. MacDonald
68k • written 4.1 years ago by
Margot
• 0
0
votes
3
replies
1.5k
views
DEseq2 extracting results from multifactor design
timecourse
DESeq2
RNASeq
updated 4.1 years ago by
Michael Love
43k • written 4.1 years ago by
Akhil
• 0
0
votes
2
replies
2.3k
views
Should immunoglobulin genes be filtered out during RNA-seq differential expression analysis
RNASeq
updated 19 months ago by
leedakyung1998
• 0 • written 4.1 years ago by
MP
• 0
0
votes
1
reply
1.2k
views
Job:
Computational Biologist at Chinook Therapeutics
RNASeq
Bioconductor
Genomics
written 4.1 years ago by
eolson
• 0
0
votes
2
replies
2.2k
views
log2FoldChange calculation in DESeq2
DESeq2
RNASeq
qPCR
qPCRData
4.1 years ago
Claire
• 0
0
votes
4
replies
1.3k
views
Clarify some basic concepts about DESeq normalization
deseq2
rnaseq
normalization
written 7.6 years ago by
xavi
• 0
0
votes
2
replies
2.1k
views
featureCounts: unable to find chromosome in SAM header
RNASeq
featureCounts
updated 3.0 years ago by
marie
• 0 • written 4.1 years ago by
nklier38
• 0
1
vote
2
replies
1.4k
views
DESeq2 log2Foldchanges opposite sign for almost the same groups of comparison
RNASeq
DESeq
4.1 years ago
krawczyk.margarita
• 0
5
votes
4
replies
11k
views
DESeq2: LRT or WALD guidance
DESeq2
RNASeq
DifferentialExpression
updated 3.5 years ago by
chm
• 0 • written 4.1 years ago by
morgan.sobol
▴ 10
0
votes
0
replies
732
views
EventPointer on TCGA RNA-seq
RNASeq
EventPointer
4.1 years ago
Marie
• 0
0
votes
5
replies
1.7k
views
DESeq2 shows comparison nonsignificant, unclear why
DESeq2
RNASeq
DifferentialExpression
updated 3.1 years ago by
Michael Love
43k • written 4.1 years ago by
daniella
• 0
4
votes
4
replies
1.6k
views
Raw RNA-seq data: column names are sequences
RNASeq
updated 4.2 years ago by
ATpoint
★ 5.0k • written 4.2 years ago by
Anne
• 0
0
votes
1
reply
858
views
PCA M3C
M3C
RNASeq
updated 4.2 years ago by
Kevin Blighe
★ 4.0k • written 4.2 years ago by
Johannes
• 0
0
votes
2
replies
1.7k
views
Deseq2 outlier detection (about Cook's distance)
DESeq2
RNASeq
DifferentialExpression
4.2 years ago
peter.zhouyu
• 0
0
votes
1
reply
1.7k
views
Identify a scRNASeq cell-population using a bulk RNA Seq dataset of that population?
RNASeq
rnaseq
scRNAseq
updated 4.2 years ago by
Aaron Lun
★ 29k • written 4.2 years ago by
curiousmind007
▴ 10
3
votes
1
reply
1.2k
views
Compare two samples by Deseqs
DESeq2
RNASeq
updated 4.2 years ago by
Kevin Blighe
★ 4.0k • written 4.2 years ago by
hwu12
▴ 10
0
votes
3
replies
1.4k
views
EdgeR question: why I got discrepancy results for DEGs when using different ways setting up the models
RNAseq
edgeR
DEGs
updated 3.6 years ago by
Gordon Smyth
53k • written 4.2 years ago by
Ming
▴ 380
0
votes
1
reply
880
views
What is the best approach to analyze RNA-seq data over 3 time points, but with average 60% missing samples in different time points?
RNASeq
updated 4.2 years ago by
James W. MacDonald
68k • written 4.2 years ago by
Jayanta Kumar
• 0
0
votes
0
replies
1.1k
views
Job:
Computational postdoctoral fellow for cancer disparities research (Boston University)
DNASeq
RNASeq
SingleCell
4.2 years ago
camp
▴ 10
2
votes
6
replies
4.3k
views
DESeq2 - understanding intercept and contrasts
DESeq2
RNASeq
4.2 years ago
sarah-spie
▴ 20
0
votes
3
replies
1.1k
views
identifying trends in RNA-seq data
RNASeq
trend
updated 4.2 years ago by
ATpoint
★ 5.0k • written 4.2 years ago by
Bogdan
▴ 670
0
votes
4
replies
1.9k
views
AlignmentsTrack empty plot
Coverage
RNASeq
plot
Gviz
updated 2.7 years ago by
James W. MacDonald
68k • written 4.2 years ago by
karlaarz
• 0
0
votes
0
replies
887
views
WGCNA out of memory
wgcna
RNASeq
4.2 years ago
zli1
• 0
0
votes
14
replies
3.6k
views
Best practice for quantifying sncRNA
Salmon
sncRNA
RNASeq
4.2 years ago
Abhishek
• 0
5
votes
9
replies
2.3k
views
Time series DE gene analysis with edgeR where specific timepoints have NA levels across different patients
edgeR
RNASeq
DifferentialExpression
TimeCourse
updated 4.2 years ago by
James W. MacDonald
68k • written 4.2 years ago by
svlachavas
▴ 840
2
votes
6
replies
5.9k
views
How can I use geom_label_repel with EnhancedVolcano?
ggrepel
RNASeq
R
EnhancedVolcano
4.2 years ago
Loren
▴ 10
1
vote
4
replies
7.2k
views
Issues with scaling down enrichplot's dotplot and gseaplot2 plots
RNASeq
clusterProfiler
enrichplot
updated 3.5 years ago by
Lihe
• 0 • written 4.2 years ago by
jiro.becker
• 0
3
votes
4
replies
1.8k
views
DRIMSeq: many NAs in results compared to DESeq2 ?
DESeq2
DRIMSeq
dtu
RNASeq
4.2 years ago
sbcn
▴ 80
0
votes
1
reply
1.1k
views
Pseudoalignment for repetitive sequences
Kallisto
RNASeq
pseudoaligners
ShortTandemRepeats
updated 4.2 years ago by
James W. MacDonald
68k • written 4.2 years ago by
Shu Yi
• 0
1
vote
3
replies
1.2k
views
subset a list based on table
edgeR
RNASeq
resfilt
updated 4.3 years ago by
ATpoint
★ 5.0k • written 4.3 years ago by
najib
• 0
2
votes
9
replies
3.3k
views
extract fold change of a list of gene from EdgeR?
EdgeR
RNAseq
Foldchange
updated 4.3 years ago by
swbarnes2
★ 1.4k • written 4.3 years ago by
najib
• 0
2
votes
1
reply
965
views
estimateSizeFactors before or after removing non-coding genes and filtering for low counts
DESeq2
RNASeq
updated 4.3 years ago by
Michael Love
43k • written 4.3 years ago by
Tash.
• 0
2
votes
1
reply
9.1k
views
EnhancedVolcano Label Question
RNASeq
EnhancedVolcano
DEGs
updated 4.3 years ago by
Kevin Blighe
★ 4.0k • written 4.3 years ago by
robledoj13
• 0
2
votes
1
reply
1.4k
views
Complex design with paired and unpaired samples with edgeR RNA-seq DE analysis
edgeR
RNASeq
updated 4.3 years ago by
Gordon Smyth
53k • written 4.3 years ago by
Basti
▴ 780
0
votes
1
reply
726
views
Creating the correct model to account for paired-samples (patient-effect) help needed!
edgeR
limma
rnaseq
updated 4.3 years ago by
Michael Love
43k • written 4.3 years ago by
22GL22
• 0
1
vote
1
reply
1.3k
views
DESeq2 for allele-specific expression with contrasts between conditions
DESeq2
AllelicImbalance
edgeR
RNASeq
updated 4.3 years ago by
Michael Love
43k • written 4.3 years ago by
Katya
• 0
0
votes
8
replies
2.8k
views
DESeq2: Variable Importance
DESeq2
RNASeq
updated 4.3 years ago by
Michael Love
43k • written 4.3 years ago by
lolol
• 0
2
votes
4
replies
1.7k
views
RNAseq : identical sample-sample distances after using limma::removeBatchEffect
DESeq2
RNASeq
BatchEffect
limma
updated 2.1 years ago by
Michael Love
43k • written 4.3 years ago by
athiebaut
• 0
0
votes
1
reply
981
views
Query regarding WGCNA module formation
WGCNA
RNAseq
RNASeqData
updated 4.4 years ago by
Lluís Revilla Sancho
▴ 760 • written 4.4 years ago by
abhisek001
• 0
0
votes
0
replies
850
views
TCSeq input information
time
RNAseq
TCSeq
4.4 years ago
curiousmind007
▴ 10
1
vote
5
replies
3.1k
views
Error in stattest function
R
rnaseq
Ballgown
updated 4.4 years ago by
muhammadharoon974
• 0 • written 7.7 years ago by
amy16
• 0
0
votes
1
reply
1.7k
views
Filtering DESeq2 results for | LFC | > 1 genes
RNAseq
LFC
DEseq
4.4 years ago
ayy2110
• 0
0
votes
2
replies
2.9k
views
Can't install KEGGprofile
KEGG
RNASeq
updated 4.4 years ago by
James W. MacDonald
68k • written 4.4 years ago by
Sophie
• 0
0
votes
2
replies
2.0k
views
Error in DESeqDataSetFromMatrix()
DESeq2
RNAseq
updated 3.3 years ago by
Luke
▴ 10 • written 4.4 years ago by
elizabethe819
• 0
0
votes
1
reply
1.1k
views
Extracting Go-enrichment and pathway analysis from phytozome annotation file
RNASeq
GO
updated 4.4 years ago by
James W. MacDonald
68k • written 4.4 years ago by
Yosapol
• 0
1,409 results • Page
7 of 29
Recent ...
Replies
Comment: PCA subset of genes vs DE
by
Ben
• 0
PCA evaluates separation according to your input. The subset of genes you used may be entirely identical in terms of expression, indicatin…
Comment: Problem with MakeTxDbFromGFF
by
Ben
• 0
Wow can't believe he can really answer this [speed stars][1] [1]: https://speed-stars.co
Comment: Help with defining groups
by
Phinney, Brett
▴ 10
I got it working , thank you!!!
Comment: Check removeBatchEffect effectiveness
by
massimo
• 0
I would kindly ask for additional support. When not including the design_timepoint argument in removeBatchEffect and thus keeping the inter…
Comment: Error Loading Experiment from SimBenchData Package
by
ksankaran
• 0
Aha, that was it! The object was class Seurat, but I didn't have it installed. After installing, everything works as expected.
Votes
Comment: Check removeBatchEffect effectiveness
Gene expression associated with continuous (quantitative) traits (Limma/edgeR/correlation)
Answer: Error Loading Experiment from SimBenchData Package
Comment: incomplete imputation of missing values with LEA impute() function
Answer: Expected memory usage for analyzing large single-cell seq datasets (> 450,000 ce
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