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Answer: How to account for solvent controls in DESeq2 when comparing follicular vs lutea
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Your approach 1 is a bad idea. Subtracting the gene counts won't do what you want to do.
I would personally check to see if there is any…
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You can always discard, although you can also retain them to check for unexpected differences.
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Yes, if I discern a batch from EDA including looking at PCs, sample correlations, etc.
It is close to SVA, we discuss both in the workflow…
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Thanks Michael. To this end, do you routinely use RUV or only when you detect a batch? It looks like it works in a similar fashion to SVA w…
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Thank you. I agree with your thoughts about the similarities of NanoString with RNA-seq. NanoString though, also has positive, negative pro…
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