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3
votes
3
replies
976
views
how to remove batch effects of RNA seq data based on continuous variables(like surrogate variables from sva package) using combatseq or limma (remove…
combatseq
RNASeqData
sva
limma
10 months ago
amnahsiddiqa
▴ 10
3
votes
8
replies
561
views
Downloading package mixOmics
mixOmics
12 weeks ago
noortje_bloem94
• 0
3
votes
1
reply
147
views
How to save the DEXSeq results
DEXSeq
updated 11 days ago by
Michael Love
42k • written 11 days ago by
Sara
• 0
3
votes
5
replies
908
views
DESeq2: Design model includes sex but only one female
DESeq2
updated 7 months ago by
Michael Love
42k • written 7 months ago by
mphogan
• 0
3
votes
4
replies
892
views
lfcShrink() and batch effects in DESeq2
BatchEffect
lfcshrink
RNA-seq
DESeq2
9 months ago
Eva
▴ 10
3
votes
4
replies
2.9k
views
Protein differential Expression analysis
differential expression
proteomics
updated 8 months ago by
ATpoint
★ 4.1k • written 6.4 years ago by
cardin.julie
• 0
3
votes
2
replies
464
views
For limma, if I leave coef=NULL, which test is the P value for?
limma
updated 10 months ago by
Gordon Smyth
50k • written 10 months ago by
S
• 0
3
votes
4
replies
8.7k
views
Gene-gene correlation analysis with RNA-seq
edgeR
updated 3 months ago by
Dimitris
• 0 • written 10.9 years ago by
Makis Motakis
▴ 30
3
votes
5
replies
667
views
DESEQ Inquiry
DESeq2
rnaseqGene
RNASeqData
limma
RNASeq
updated 10 weeks ago by
Michael Love
42k • written 12 weeks ago by
sap275
▴ 10
3
votes
6
replies
716
views
Methylation data analysis
MethylationArrayData
methylationArrayAnalysis
6 months ago
aldea85
• 0
3
votes
5
replies
1.7k
views
Error: 'IdClusters'
DECIPHER
updated 11 months ago by
Ling-Hsiu
▴ 10 • written 15 months ago by
julieta.micorriza
• 0
3
votes
2
replies
581
views
tximport: Error in is(txi, "list") : argument "txi" is missing, with no default
tximport
DESeq2
updated 5 months ago by
James W. MacDonald
65k • written 5 months ago by
mckinlee.salazar
• 0
3
votes
1
reply
440
views
NPARC package y-variable
NPARC
NPA
updated 8 months ago by
nils.kurzawa
▴ 20 • written 8 months ago by
Aidan
• 0
3
votes
4
replies
1.2k
views
Annotation hub and clusterProfiler errors
clusterProfiler
Annotationhub
updated 6 months ago by
Guido Hooiveld
★ 4.0k • written 6 months ago by
BS
• 0
3
votes
3
replies
9.5k
views
How to find rsID with biomaRt in R
biomaRt
updated 3 months ago by
James W. MacDonald
65k • written 3.2 years ago by
Federica
▴ 10
3
votes
7
replies
1.9k
views
Installing densvis on a Singularity container
densvis
docker
updated 11 months ago by
alan.ocallaghan
▴ 160 • written 12 months ago by
James W. MacDonald
65k
3
votes
5
replies
891
views
Getting batch corrected counts after RUVSeq
BatchEffect
RUVSeq
RNASeq
DESeq2
updated 8 months ago by
ATpoint
★ 4.1k • written 8 months ago by
Shashank
• 0
3
votes
8
replies
930
views
DropletUtils read10xcounts adds the 12 Cell Multiplexing Oligo tags as gene names
DropletUtils
droplet
written 5 months ago by
pcantalupo
▴ 10
3
votes
6
replies
23k
views
Deseq: What does "BaseMean" mean?
deseq2
updated 4 months ago by
kledo577
• 0 • written 5.7 years ago by
183227851
• 0
3
votes
2
replies
688
views
Does this RNAseq experiment require a repeated measures approach?
StatisticalMethod
RNASeqData
edgeR
DESeq2
updated 10 months ago by
Gordon Smyth
50k • written 10 months ago by
CDSPARKS
▴ 10
3
votes
3
replies
714
views
EdgeR: Should I include all samples/conditions in my dispersion estimation while only testing for certain contrasts
edgeR
updated 4 months ago by
Gordon Smyth
50k • written 4 months ago by
Jurgen
• 0
3
votes
6
replies
2.2k
views
Result from GSEA for non-model organism not as expected
clusterProfiler
updated 5 months ago by
James W. MacDonald
65k • written 19 months ago by
Susanne
▴ 10
3
votes
5
replies
2.2k
views
Why the LogFC and delta-beta value are different in ChAMP
DNAMethylation
ChAMP
updated 12 weeks ago by
sup0903
• 0 • written 3.4 years ago by
Hongbii
• 0
3
votes
2
replies
496
views
How does one test for interactions in EdgeR?
edgeR
3 months ago
pl23
• 0
3
votes
5
replies
924
views
limma-trend vs. limma-voom theoretical difference
limma
limma-trend
limma-voom
updated 4 months ago by
floriandeckert
▴ 30 • written 18 months ago by
hainct
• 0
3
votes
11
replies
5.0k
views
GO enrichment analysis on Solanum lycopersicum proteomics dataset (UniProt IDs)
clusterProfiler
AnnotationHub
GO
uniprot
updated 3 months ago by
Guido Hooiveld
★ 4.0k • written 3.3 years ago by
Alberto
• 0
3
votes
10
replies
2.8k
views
error in package GenVisR while installing
GenVisR
GenVis
updated 6 months ago by
zlskidmore
▴ 30 • written 22 months ago by
abhisikta
• 0
3
votes
6
replies
860
views
Question on constructing design matrix and defining contrasts for analysis
wgcna
DESeq2
edgeR
limma
design
updated 11 months ago by
Gordon Smyth
50k • written 11 months ago by
mohammedtoufiq91
▴ 10
3
votes
16
replies
18k
views
featureCounts - paired-end data
featurecounts
rsubread
updated 11 months ago by
Robert
• 0 • written 9.0 years ago by
samuel collombet
▴ 140
3
votes
4
replies
260
views
Help with error using camera() in limma
CAMERA
limma
17 days ago
Chris
▴ 10
3
votes
4
replies
505
views
Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it does not happen in Linux
GenomAutomorphism
updated 9 days ago by
Mike Smith
★ 6.5k • written 12 days ago by
robersy
• 0
3
votes
4
replies
506
views
Right way to pseudobulk donors across replicates in edgeR
pseudobulk
edgeR
DifferentialExpression
updated 10 weeks ago by
Gordon Smyth
50k • written 10 weeks ago by
Jack S.
▴ 50
3
votes
2
replies
478
views
What is the meaning of "CpG interrogation" and "single nucleotide extension(SBE)"?
SBE
CpG
minfi
CpGinterrogation
5 months ago
kyj2226
• 0
3
votes
5
replies
288
views
Help with running egsea()
EGSEA
11 days ago • updated 10 days ago
Chris
▴ 10
3
votes
18
replies
3.0k
views
News:
List of Deprecated Packages for Bioc3.18
Deprecation
devel
Bioconductor
Devel
10 months ago • updated 4 weeks ago
shepherl
3.9k
3
votes
2
replies
234
views
How to extract counts from a DESeq dataset ordered by a specific sample?
DESeq2
Transcriptomics
7 weeks ago
Anita
▴ 10
3
votes
11
replies
1.4k
views
SD filtering before DEG analysis with limma : possible or not ?
filtering
STD
DEG
limma
updated 10 months ago by
Gordon Smyth
50k • written 10 months ago by
alexia.brunel
• 0
3
votes
2
replies
407
views
How to do DESeq2 on Kallisto Data
DESeq2
Kallisto
updated 3 months ago by
dsull
▴ 30 • written 3 months ago by
Aaliya
• 0
3
votes
1
reply
328
views
Use of negative binomial model for exponential decay rate estimation using DESeq (or similar)
DESeq2
updated 24 days ago by
Michael Love
42k • written 24 days ago by
i.sudbery
▴ 40
3
votes
3
replies
13k
views
How to fix 'long vectors not supported yet' error in R
r
updated 6 weeks ago by
Amruta
• 0 • written 5.3 years ago by
r.tor
• 0
3
votes
3
replies
454
views
Function 'sexp_as_cholmod_sparse' doesn't exist in the package 'Matrix'
scater
runPCA
updated 7 weeks ago by
alan.ocallaghan
▴ 160 • written 7 weeks ago by
Najla Abassi
• 0
3
votes
1
reply
1.7k
views
Is it always preferable to recount library sizes of DGEList object in edgeR after filtering?
edgeR
RnaSeqSampleSizeData
RNAseq
updated 4 months ago by
Gordon Smyth
50k • written 3.0 years ago by
Lucy
▴ 60
3
votes
16
replies
2.2k
views
DESeq2 - groups with biological replicates from the same patient
DESeq2
updated 3 months ago by
shepherl
3.9k • written 2.9 years ago by
S
▴ 10
3
votes
6
replies
305
views
Surrogate variable analysis for paired RNA seq experiment
RUVSeq
sva
5 weeks ago
nhaus
▴ 30
3
votes
1
reply
1.4k
views
Mouse to Human Genes using bioconductor
ensembldb
updated 9 months ago by
James W. MacDonald
65k • written 9 months ago by
Max
▴ 10
3
votes
1
reply
327
views
Dataset imbalance
RNASeq
DifferentialExpression
RnaSeqSampleSizeData
RnaSeqSampleSize
updated 4 months ago by
Gordon Smyth
50k • written 4 months ago by
Shaimaa Gamal
• 0
3
votes
4
replies
696
views
Questions Trying to Re-create MicroArray Data (GSE97562) (Fig. 2)
MicroRNAArrayData
GSE97562
pd.hugene.1.0.st.v1
Annotation
updated 10 months ago by
James W. MacDonald
65k • written 10 months ago by
rodj5201
• 0
3
votes
12
replies
2.2k
views
t.test to determine the P-value in DESeq2
DESeq2
written 19 months ago by
Maryam.sed1400
• 0
3
votes
7
replies
882
views
Genes in open chromatin region
ChIPpeakAnno
DiffBind
written 8 months ago by
Chris
▴ 10
3
votes
3
replies
250
views
DESeq2 - dispersion estimation when there is none
DESeq2
updated 24 days ago by
Michael Love
42k • written 25 days ago by
WardDeb
• 0
1,738 results • Page
4 of 35
Recent ...
Replies
Comment: Numerical differences in DESeq2 output depending on different design matrix cons
by
JKim
• 0
The 1st model has an interaction term, but the 2nd model doesn't have the interaction term. That would probably make the difference.
Comment: Ensembl plants at annotationHub?
by
Daniele
• 0
Hi, I'm using Ensembl Plants release 59 with the IWGSC RefSeq v1.1 gene annotation. Thanks a lot for replying so quickly!
Comment: Numerical differences in DESeq2 output depending on different design matrix cons
by
swbarnes2
★ 1.4k
If you restrict yourself to, say, genes with a baseMean > 50, are the numerical differences between the two designs significant?
Comment: DESeq2- Mysterious interaction suppresses DEGs
by
shaunpeterson
• 0
The images I tried to attach didn't attach. Here is the PCA (MDS) of all the samples, with the Cortex outlier highlighed. ![enter image de…
Comment: STRINGdb : error with string_db$plot_network()
by
damian.szk
▴ 80
ok. Forcing non-secure protocol should not be needed anymore, due to changes in the underlying library. However, I will look at why "HTTP" …
Votes
Answer: Error while installing BiocManager::install("org.Vdahliae.eg.db")
Comment: How can I create a eigencorplot of pca created from deseq2 object with PCAtools
Answer: Importing a GraphNEL into Cytoscape with RCy3 with error: "Error in cyrestPUT(pa
Answer: Importing a GraphNEL into Cytoscape with RCy3 with error: "Error in cyrestPUT(pa
Rsubread counting peaks ATACseq
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