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votes
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135
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putting my own expression data in deseq2
DESeq2
updated 17 days ago by
ATpoint
★ 4.1k • written 17 days ago by
Dev
• 0
2
votes
1
reply
62
views
How to design DEXSeq formula for multiple variables
DEXSeq
updated 2 days ago by
Alejandro Reyes
★ 1.9k • written 4 days ago by
Sara
• 0
1
vote
7
replies
355
views
Math expression in shapeCustom legend in EnhancedVolcano
EnhancedVolcano
22 days ago • updated 21 days ago
pl23
• 0
0
votes
1
reply
136
views
FDR via q-value
qvalue
updated 25 days ago by
James W. MacDonald
65k • written 28 days ago by
John
• 0
0
votes
7
replies
361
views
Methylation Analysis experiment design using Limma
DNAMethylation
limma
methylationArrayAnalysis
4 weeks ago • updated 24 days ago
hortense96
• 0
15
votes
23
replies
2.0k
views
The difference when using Limma compare between 3 conditions vs 2 conditions
limma
7 weeks ago • updated 17 days ago
Chris
▴ 10
1
vote
4
replies
229
views
minfi::read.metharray.exp
minfiData
minfi
updated 23 days ago by
Basti
▴ 780 • written 25 days ago by
clacarion
• 0
4
votes
9
replies
3.1k
views
Create protein sequences including variants from a VCF file
VariantAnnotation
customProDB
updated 2 days ago by
Yun
• 0 • written 5.2 years ago by
daniel.magnus.bader
▴ 50
0
votes
4
replies
366
views
More regions in union when increasing DBA$config$mergeOverlap
DiffBind
updated 6 days ago by
Ziliang
• 0 • written 16 days ago by
Ian D.
▴ 70
1
vote
3
replies
235
views
Different Shrunk Log2FC according with reference
DESeq2
updated 23 days ago by
Michael Love
42k • written 23 days ago by
andrebolerbarros
▴ 20
1
vote
2
replies
1.0k
views
DMR.Plot shows error "Error in simpleLoess(y, x, w, span, degree = degree, parametric = FALSE, : NA/NaN/Inf in foreign function call (arg 1)"
DMRcate
Gviz
updated 24 days ago by
Tim Peters
▴ 180 • written 14 months ago by
jose.ramirez1
▴ 10
3
votes
1
reply
328
views
Use of negative binomial model for exponential decay rate estimation using DESeq (or similar)
DESeq2
updated 23 days ago by
Michael Love
42k • written 24 days ago by
i.sudbery
▴ 40
6
votes
10
replies
1.7k
views
Which input file is used for DGEList in EgdeR?
salmon
edgeR
tximport
nf-core
gene_counts
updated 28 days ago by
i.atanasoai
• 0 • written 15 months ago by
mohammedtoufiq91
▴ 10
1
vote
5
replies
1.5k
views
Cannot connect to ExperimentHub server
ExperimentHub
updated 9 days ago by
Jiayu
• 0 • written 5 months ago by
yanling
• 0
0
votes
2
replies
207
views
Deseq2 and Normalization of RNA sequencing data
DESeq2
16 days ago • updated 15 days ago
kcarey
• 0
2
votes
1
reply
141
views
Trim/Filter out-of-bounds GRanges
GenomicRanges
updated 11 days ago by
Michael Love
42k • written 11 days ago by
mat149
▴ 70
2
votes
3
replies
111
views
How to correctly adjust for gender in edgeR
design
glm
edgeR
updated 2 days ago by
Gordon Smyth
50k • written 2 days ago by
Guillermo
• 0
1
vote
2
replies
287
views
Get genomic coordinates of CpGs sites (chromosomes, genomic position)
GenomeWideAssociation
SequenceAnnotation
MethylationArray
GenomicDistributionsData
16 days ago • updated 15 days ago
Yveto
• 0
2
votes
2
replies
342
views
Importing a GraphNEL into Cytoscape with RCy3 with error: "Error in cyrestPUT(paste("networks", net.suid, "tables", tbl, sep = "/"), : object 're…
RCy3
5 days ago • updated 1 day ago
Matthew Thornton
▴ 360
0
votes
1
reply
97
views
normalization using Deseq2
DESeq2
rnaseqGene
Normalization
updated 8 days ago by
ATpoint
★ 4.1k • written 8 days ago by
sajadahmad41454
• 0
2
votes
5
replies
258
views
How to retrieve gene ontology GO class from gene list?
GO.db
goTools
updated 22 days ago by
James W. MacDonald
65k • written 24 days ago by
bandconductor
• 0
0
votes
3
replies
210
views
Finding DEGs by using edgeR
subsettingdata
edgeR
designmatrix
updated 28 days ago by
Gordon Smyth
50k • written 29 days ago by
prity6459
• 0
0
votes
7
replies
445
views
DESeq filtering specific to contrasts
DESeq2
updated 28 days ago by
Michael Love
42k • written 3 months ago by
Emma
• 0
0
votes
1
reply
155
views
rlog transformation
DESeq2
updated 29 days ago by
Michael Love
42k • written 29 days ago by
ishij9717
• 0
0
votes
2
replies
928
views
Problem for loading Bioconductor package in R
Bioconductor
written 4.3 years ago by
araaaju
• 0
0
votes
2
replies
237
views
RNAseq:Technical replicates from different batches
technicalReplicates
BatchEffect
limma
RNASeq
28 days ago • updated 25 days ago
catv
• 0
1
vote
3
replies
302
views
Getting Error in hclust(d, method = method): NA/NaN/Inf in foreign function call when trying to subset pheatmap
DESeq2
updated 9 days ago by
ATpoint
★ 4.1k • written 16 days ago by
angelathuynh5
• 0
0
votes
2
replies
946
views
using WGCNA, positive or negative corrlation between genes composing modules.
correlation
wgcna
network
updated 2 days ago by
mariakenneth147
• 0 • written 17 months ago by
pharamac
• 0
0
votes
3
replies
273
views
Assist me in determining whether the analysis process using the limma package has been executed correctly.
GEOquery
ArrayExpress
limma
DifferentialExpression
updated 17 days ago by
James W. MacDonald
65k • written 18 days ago by
SSSJec
• 0
1
vote
5
replies
514
views
Can't post a question containing code?
Bioconductor
updated 7 days ago by
NATASHA
• 0 • written 3 months ago by
DS
▴ 10
5
votes
6
replies
2.8k
views
Although I successfully installed the package "simpleaffy'' , I cannot library it. Why?
simpleaffy
updated 15 days ago by
James W. MacDonald
65k • written 2.3 years ago by
yanchangjian2019
• 0
5
votes
8
replies
8.8k
views
Limma design with continuous and categorical covariates
limma
batch effect
covariates
microarray
updated 24 days ago by
hortense96
• 0 • written 7.8 years ago by
dmcleanuk
▴ 30
0
votes
3
replies
98
views
Assymetric MA plot with no overall trend
DESeq2
DifferentialExpression
updated 2 days ago by
Michael Love
42k • written 3 days ago by
Kasper
• 0
1
vote
1
reply
131
views
Cannabis OrgDb
KEGGgraph
org.Cs.eg.db
clusterProfiler
KEGG
AnnotationHubSoftware
updated 24 days ago by
James W. MacDonald
65k • written 24 days ago by
fernanda.backsouza
▴ 10
4
votes
6
replies
467
views
FilterByExpr low counts with small sample size
edgeR
4 weeks ago • updated 25 days ago
Jonathan
▴ 10
0
votes
1
reply
154
views
Gene-by-sample covariates in limma
Metabolomics
limma
updated 15 days ago by
Gordon Smyth
50k • written 15 days ago by
c53aba27
• 0
0
votes
4
replies
216
views
FGSEA pre-ranked input in increasing order or decreasing
fgsea
29 days ago
sropri
• 0
0
votes
1
reply
158
views
Differential gene expression analysis results
DESeq2
DifferentialExpression
updated 7 days ago by
ATpoint
★ 4.1k • written 7 days ago by
sajadahmad41454
• 0
1
vote
2
replies
738
views
DESeq2 Error in `.rowNamesDF<-`(x, value = value): Invalid 'row.names' length
DESeq2
4 weeks ago • updated 21 days ago
Patadù
▴ 10
0
votes
2
replies
190
views
limma-voom: Calculate the log-fold change in gene expression within a sample
limma
RNASeq
voom
written 25 days ago by
taur.vil
• 0
2
votes
5
replies
790
views
DESeq2 setting significant p-value
DESeq2
updated 28 days ago by
Michael Love
42k • written 5.0 years ago by
Merlin
▴ 10
0
votes
1
reply
172
views
NEED FOR NESSASORY HELP
RNASeqData
edgeR
updated 15 days ago by
Gordon Smyth
50k • written 15 days ago by
z_bahari
• 0
2
votes
3
replies
89
views
Problem installing ChAMP on a MacBook
ChAMP
updated 1 day ago by
shepherl
3.9k • written 1 day ago by
Galia
• 0
1
vote
2
replies
366
views
CombineArrays for EPIC and EPIC V2
EPICV1
Methylation
combineArrays
minifi
EPICv2manifest
6 weeks ago • updated 14 days ago
Kim
• 0
2
votes
2
replies
1.1k
views
limma for gene expression analysis with big and unbalanced sample size
limma
microarray
statistics
updated 17 days ago by
XW
• 0 • written 8.0 years ago by
tati6406
• 0
1
vote
4
replies
355
views
Opposite sign of LFC in count plots of DEGs (DESeq2)
DESeq2
23 days ago • updated 21 days ago
winwater0928
• 0
2
votes
2
replies
223
views
fgsea significant result
fgsea
13 days ago • updated 12 days ago
Chris
▴ 10
0
votes
3
replies
300
views
log2FoldChange value is way too different when compared with counts(dds)
DESeq2
updated 16 days ago by
Michael Love
42k • written 17 days ago by
HAK
• 0
2
votes
2
replies
268
views
error in limma , contrast.matrix Number of rows of contrast matrix must match number of coefficients in fit
limma
10 days ago • updated 4 days ago
zzrammal
• 0
3
votes
1
reply
147
views
How to save the DEXSeq results
DEXSeq
updated 11 days ago by
Michael Love
42k • written 11 days ago by
Sara
• 0
192 results • Page
2 of 4
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Comment: Ensembl plants at annotationHub?
by
Daniele
• 0
Hi, I'm using Ensembl Plants release 59 with the IWGSC RefSeq v1.1 gene annotation. Thanks a lot for replying so quickly!
Comment: Numerical differences in DESeq2 output depending on different design matrix cons
by
swbarnes2
★ 1.4k
If you restrict yourself to, say, genes with a baseMean > 50, are the numerical differences between the two designs significant?
Comment: DESeq2- Mysterious interaction suppresses DEGs
by
shaunpeterson
• 0
The images I tried to attach didn't attach. Here is the PCA (MDS) of all the samples, with the Cortex outlier highlighed. ![enter image de…
Comment: STRINGdb : error with string_db$plot_network()
by
damian.szk
▴ 80
ok. Forcing non-secure protocol should not be needed anymore, due to changes in the underlying library. However, I will look at why "HTTP" …
Comment: STRINGdb : error with string_db$plot_network()
by
ninidea
• 0
> Does the vignette produce the same error? No. Anyway, I finally solved the problem. I don't know why, but I removed: protocol='http' fr…
Votes
Answer: Error while installing BiocManager::install("org.Vdahliae.eg.db")
Comment: How can I create a eigencorplot of pca created from deseq2 object with PCAtools
Answer: Importing a GraphNEL into Cytoscape with RCy3 with error: "Error in cyrestPUT(pa
Answer: Importing a GraphNEL into Cytoscape with RCy3 with error: "Error in cyrestPUT(pa
Rsubread counting peaks ATACseq
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