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votes
0
replies
17
views
gsameth GSA: probe annotation to multiple genes
missMethyl
gsameth
2 hours ago
nurkenber
• 0
0
votes
0
replies
16
views
Testing Differential Expression with Interaction Effect Between Categorical and Continuous Variable with Repeated (Paired) Measures using variancePar…
limma
variancePartition
6 hours ago
GJ
• 0
0
votes
1
reply
38
views
promoters_txdb <- promoters(txdb, upstream = 2000) is not 2000
TxDb.Hsapiens.UCSC.hg38.knownGene
updated 6 hours ago by
James W. MacDonald
67k • written 14 hours ago by
linouhao
▴ 20
0
votes
0
replies
25
views
News:
Online course: Gene set enrichment analysis in R/Bioconductor
GSEABenchmarkeR
1 day ago
info
▴ 10
0
votes
1
reply
87
views
org.Hs.eg.db issue
org.Hs.eg.db
updated 3 hours ago by
shepherl
4.1k • written 2 days ago by
k.panov
• 0
0
votes
2
replies
320
views
Paired analysis in proteomics data for DE while interested in only a particular subset of the data
limma
modelmatrix
pairedanalysis
Proteomics
written 3 days ago by
svlachavas
▴ 840
0
votes
2
replies
164
views
iam not able to do retrive data using GEOquery R package
HELP
updated 3 days ago by
James W. MacDonald
67k • written 3 days ago by
ramya20kumar20
• 0
2
votes
4
replies
206
views
Differential variability modelling with vst() expression
limma
DESeq2
updated 3 days ago by
Michael Love
43k • written 4 days ago by
jtsy6383
• 0
0
votes
2
replies
100
views
Installation error for Rsamtools.
Rsamtools
updated 3 days ago by
ATpoint
★ 4.6k • written 4 days ago by
Sheila
• 0
2
votes
8
replies
430
views
Different annotation packages (org.Mm.eg.db, EnsDb.Mmusculus.v79, BiomaRt) returning drastically different numbers of transcripts
AnnotationDbi
EnsDb.Mmusculus.v79
biomaRt
org.Mm.eg.db
updated 3 days ago by
James W. MacDonald
67k • written 4 days ago by
Nicholas
• 0
0
votes
2
replies
251
views
DESeq2 analysis with transcript counts
Transcriptomics
RNASeq
DESeq2
4 days ago • updated 2 days ago
sindhuri
• 0
0
votes
0
replies
40
views
Issue when attempting to use output from DECIPHER IDtaxa (pr2 V5) with dada2/phyloseq
DECIPHER
taxonomyassignment
phyloseq
dada2
Pr2V5
5 days ago
Maggie
• 0
2
votes
8
replies
532
views
Why did PCA plots look exactly the same after adjusting for batch covariate in DESeq2 design?
DESeq2
BatchEffect
5 days ago • updated 3 days ago
Quang
▴ 10
0
votes
5
replies
197
views
Diffbind interpretation of data - fold change
DiffBind
6 days ago
Vera
• 0
1
vote
1
reply
250
views
Considerations behind the choice of RNAseq Differential Expression Analysis Tools
fishpond
DESeq2
rnaseqDTU
updated 6 days ago by
Michael Love
43k • written 6 days ago by
muhammadsohailraza
• 0
1
vote
2
replies
211
views
Package `not available for Bioconductor version '3.20'` when it should be
consensusOV
genefu
6 days ago
Levi Waldron
★ 1.1k
0
votes
1
reply
62
views
No preprocessing allowed for EPICv2 with DMRcate
missMethyl
DMRcate
updated 6 days ago by
James W. MacDonald
67k • written 6 days ago by
maximuscfyeung
• 0
0
votes
0
replies
39
views
Trouble with double delimiters in .fcs file (flowCore package)
flowCore
FlowCytometry
FlowCytometryData
7 days ago
vpochic
• 0
0
votes
1
reply
121
views
Want numbers in DESeq to be seen as numerical, not categorical
DESeq2
updated 7 days ago by
swbarnes2
★ 1.4k • written 7 days ago by
natalievillafranca
• 0
0
votes
4
replies
191
views
Newbie Question - adding metadata to an analysis
Biobase
7 days ago • updated 5 days ago
Bryan H
• 0
0
votes
2
replies
207
views
MissMethyl failed to use gometh function for EPICv2
missMethyl
EPICv2manifest
updated 7 days ago by
James W. MacDonald
67k • written 7 days ago by
maximuscfyeung
• 0
0
votes
1
reply
91
views
Correlation between gene clusters
genomes
RNASeqData
written 7 days ago by
Le
• 0
0
votes
2
replies
169
views
Mean variance curve troubleshoot filtering
limmavoom
filtering
edgeR
RNASeqData
meanvariancetrend
9 days ago • updated 7 days ago
gingergeiger22
• 0
0
votes
0
replies
57
views
Error ("could not find function "EM_estimate"") when running ENmix mpreprocess() on Microsoft Azure
Preprocessing
ENmix
9 days ago
balf0020
• 0
6
votes
5
replies
507
views
How do I fix an odd voom plot in a combined dataset?
limma
Normalization
voom
updated 6 days ago by
James W. MacDonald
67k • written 12 days ago by
1sunmic2
• 0
1
vote
8
replies
767
views
Incorporating Salmon offset matrix into Wilcoxon DEG analysis
edgeR
13 days ago • updated 6 days ago
Andre
• 0
0
votes
2
replies
236
views
DESeq error: counts matrix should be numeric, currently it has mode: character
DESeq2
15 days ago • updated 14 days ago
Kucsera
• 0
2
votes
1
reply
327
views
Is it possible to work with DESeq2 where data is not properly matched for sex and age
DESeq2
RNASeqData
updated 10 days ago by
Michael Love
43k • written 17 days ago by
KR
• 0
0
votes
1
reply
160
views
combining raw counts and estimated counts
RNASeqData
GEO
TCGAbiolinks
updated 18 days ago by
O'Brien
• 0 • written 20 days ago by
Noor
• 0
0
votes
1
reply
320
views
Please, I desperately need help with DESeq2. Three groups of repeated measures with two timepoints... (plus an additional group)
edgeR
DESeq2
updated 10 days ago by
Michael Love
43k • written 20 days ago by
Jimmy Mak
• 0
0
votes
2
replies
429
views
Cross-validation with multiple control subgroups in limma
limma
multtest
24 days ago • updated 22 days ago
Ali Barry
▴ 40
0
votes
0
replies
84
views
Job:
Postdoctoral Researcher in Spatial Gene Regulatory Networks underlying Stomatal Patterning
JobPosting
25 days ago
Jenny Drnevich
★ 2.0k
0
votes
1
reply
310
views
ChAMP.DMR error - Error in makeGenomicRatioSetFromMatrix
ChAMP
updated 18 days ago by
O'Brien
• 0 • written 26 days ago by
André
• 0
4
votes
8
replies
1.5k
views
Issues with seqlevelsStyle when making custom txdb objects for genomes/annotations from ToxoDB
txdbmaker
GenomeInfoDb
GenomicFeatures
Biostrings
gDNAx
updated 23 days ago by
Robert Castelo
★ 3.4k • written 26 days ago by
rohitsatyam102
▴ 20
1
vote
9
replies
761
views
Normalizing batch effects using technical replicates for DGE analysis between two conditions from 2 different runs
limma
DESeq2
edgeR
written 26 days ago by
anna.cot.anna.cot
▴ 30
0
votes
2
replies
218
views
Error in get_anno_data(ontology) : ontology not supported yet...
EnrichDO
27 days ago • updated 26 days ago
GODseeker
• 0
0
votes
2
replies
253
views
Build error on kunpeng2 Linux aarch64
Bioconductor
updated 12 days ago by
wise
• 0 • written 28 days ago by
Hongxiang
• 0
0
votes
1
reply
153
views
Errors running learn.bn function from Pigengene in windows environment
Pigengene
written 4 weeks ago by
Thomas
• 0
0
votes
1
reply
257
views
Need new version : tx2gene.gencode.v47.csv
tximportData
updated 4 weeks ago by
Guido Hooiveld
★ 4.1k • written 4 weeks ago by
gauravbcbp22
• 0
1
vote
5
replies
519
views
Deseq2 Design
RNASeq
DESeq2
updated 28 days ago by
James W. MacDonald
67k • written 4 weeks ago by
pm_25
• 0
0
votes
2
replies
284
views
Standardization of rlog transformed counts before PCA
RNASeq
DESeq2
Visualization
updated 27 days ago by
ATpoint
★ 4.6k • written 4 weeks ago by
bbao
• 0
0
votes
5
replies
483
views
p value is 0, padj is 0
DESeq2
4 weeks ago
Wirayatida
• 0
0
votes
2
replies
436
views
Cannot install GO.db and org.Hs.eg.db
Install
updated 4 weeks ago by
James W. MacDonald
67k • written 4 weeks ago by
Gabriel
• 0
0
votes
5
replies
594
views
Help using rtracklayer for gene ID extraction
rtracklayer
written 4 weeks ago by
Aichatou
• 0
1
vote
2
replies
469
views
Pre-filtering TPM kallisto for ssGSEA / DESeq2
tximport
DESeq2
GSVA
4 weeks ago
georgii.vdovin
• 0
1
vote
1
reply
351
views
Can DESeq2's design compensate for sequencing experimental design shortcomings?
DESeq2
updated 24 days ago by
Michael Love
43k • written 4 weeks ago by
txema.heredia
• 0
0
votes
0
replies
10
views
Spam:
Best Hotels In Maui
prebs
4 weeks ago
2zrcj3448p
0
0
votes
1
reply
9
views
Spam:
Small Business Directory Emerald Coast
BUScorrect
offtopic
updated 4 weeks ago by
shepherl
4.1k • written 4 weeks ago by
2zrcj3448p
0
0
votes
1
reply
154
views
Matching patterns in an AAStringset
Biostrings
updated 4 weeks ago by
Aidan
▴ 60 • written 4 weeks ago by
Cédric
• 0
4
votes
8
replies
1.1k
views
Feature Counts - potential bug with outfile.jcounts
featurecounts
Rsubread
4 weeks ago • updated 26 days ago
chris2.a.white
• 0
1,461 results • Page
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Answer: promoters_txdb <- promoters(txdb, upstream = 2000) is not 2000
by
James W. MacDonald
67k
If you use `promoters` and do not provide an argument for something that has a default, you will get the default argument. Your last call t…
Comment: org.Hs.eg.db issue
by
k.panov
• 0
installed R on another PC, works perfectly. ergo, problem outside of R probably..... :-(
Comment: MBDseq analysis using qsea package
by
Lukas
▴ 20
Hi, I checked in with Matthias and here is his response: You need to indicate "samples": "The indices of the samples for which individu…
Comment: DESeq2 analysis with transcript counts
by
sindhuri
• 0
Thank you for the response. Currently I am using the R studio for analysis and Im running DESeq2 for analysis of the **transcript counts.ts…
Comment: iam not able to do retrive data using GEOquery R package
by
James W. MacDonald
67k
Another alternative is to use the `GEOmetadb` package ([vignette here)][1], which will allow you to make queries to identify GEO datasets t…
Votes
Answer: Is there any chance to solve "the model matrix is not full rank" in DESeq2?
Answer: Issue carrying out DiffBind Analysis at a predefined peak set
Answer: Differential variability modelling with vst() expression
Answer: Differential variability modelling with vst() expression
Comment: Different annotation packages (org.Mm.eg.db, EnsDb.Mmusculus.v79, BiomaRt) retu
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