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votes
0
replies
4
views
putting my own expression data in deseq2
DESeq2
20 minutes ago
Dev
• 0
0
votes
0
replies
15
views
Timecourse RNASeq analysis
DESeq2
ImpulseDE2
timecour
timecoursedata
moanin
2 hours ago
Aurora
• 0
0
votes
0
replies
12
views
Version 3.15 S4Vectors packages are missing
S4Vectors
5 hours ago
bounlu
• 0
0
votes
2
replies
97
views
Assist me in determining whether the analysis process using the limma package has been executed correctly.
GEOquery
ArrayExpress
limma
DifferentialExpression
19 hours ago • updated 6 hours ago
SSSJec
• 0
0
votes
0
replies
10
views
How do I select a GC file (gc.wig)? (How to determine the inputdata of HMMcopy)
CopyNumberPlots
HMMcopy
CopyNumberVariation
6 hours ago
SSSJec
• 0
13
votes
16
replies
1.0k
views
Why p value inconsistent when using different number of pathway score?
limma
updated 9 hours ago by
Gordon Smyth
50k • written 9 days ago by
Chris
• 0
2
votes
2
replies
108
views
Perform gene set enrichment analysis using fgsea on two conditions that don't have differential express genes
fgsea
15 hours ago • updated 13 hours ago
Chris
• 0
0
votes
0
replies
20
views
Padj "NA" with thousands of low count although the raw read counts are quite large
DESeq2
13 hours ago
Agung
• 0
0
votes
0
replies
30
views
Could we detect difference at pathway level but not significant different at gene level?
GSVA
19 hours ago • updated 16 hours ago
Chris
• 0
6
votes
17
replies
2.3k
views
DESeq2 a lot of genes showing up as differentially expressed that only have 1 sample with any expression
deseq2
updated 17 hours ago by
Seyram M.
• 0 • written 4.6 years ago by
hsbio
▴ 10
0
votes
4
replies
228
views
Minfi - estimatecellscount
min
minfi
updated 19 hours ago by
James W. MacDonald
65k • written 4 weeks ago by
clacarion
• 0
8
votes
5
replies
1.6k
views
SGSeq: moving toward diffex from SGSeq analysis
sgseq
rnaseq
updated 20 hours ago by
Sara
• 0 • written 6.7 years ago by
Sylvain Foisy
▴ 80
3
votes
3
replies
155
views
DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
DESeq2
contrasts
updated 1 day ago by
ATpoint
★ 4.1k • written 1 day ago by
bioinf
▴ 10
0
votes
2
replies
127
views
Different number of exons in reference genome affects DEXSeq analysis
DEXSeq
11 days ago • updated 1 day ago
Alessia
• 0
0
votes
0
replies
58
views
how to do Differential splicing from SGSeq analysis
DEXSeq
SGSeq
5 days ago • updated 1 day ago
Sara
• 0
1
vote
1
reply
93
views
Dominance Index for Shotgun Metagenomics Data
microbiome
updated 3 days ago by
Leo Lahti
▴ 40 • written 3 days ago by
Dario Strbenac
★ 1.5k
1
vote
7
replies
252
views
Math expression in shapeCustom legend in EnhancedVolcano
EnhancedVolcano
4 days ago • updated 3 days ago
pl23
• 0
0
votes
2
replies
154
views
PCA plot suggestions
DESeq2
PCAtools
updated 3 days ago by
Michael Love
41k • written 5 days ago by
Aaliya
• 0
1
vote
2
replies
681
views
DESeq2 Error in `.rowNamesDF<-`(x, value = value): Invalid 'row.names' length
DESeq2
12 days ago • updated 3 days ago
ussarizona
▴ 10
1
vote
3
replies
163
views
Deseq2. decontXcounts not integers. Useful alternative?
DESeq2
celda
updated 3 days ago by
Michael Love
41k • written 4 days ago by
JalapenoCornbread
• 0
0
votes
1
reply
92
views
ATACseq merge peaks
DiffBind
updated 3 days ago by
ATpoint
★ 4.1k • written 5 days ago by
Shloka
• 0
1
vote
1
reply
133
views
Improving the design matrices vignette in RNAseq123
RNAseq123
updated 4 days ago by
Gordon Smyth
50k • written 8 days ago by
Lluís Revilla Sancho
▴ 730
1
vote
4
replies
271
views
Opposite sign of LFC in count plots of DEGs (DESeq2)
DESeq2
6 days ago • updated 4 days ago
winwater0928
• 0
2
votes
9
replies
321
views
Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
DESeq2
RUVSeq
RUVseq
BatchEffect
DES
updated 4 days ago by
James W. MacDonald
65k • written 4 days ago by
Alexandre
• 0
2
votes
5
replies
201
views
How to retrieve gene ontology GO class from gene list?
GO.db
goTools
updated 4 days ago by
James W. MacDonald
65k • written 6 days ago by
bandconductor
• 0
0
votes
0
replies
60
views
One error problem occurred caused by data type during using GDCprepare to handle BRCA data
GDCRNATools
TCGA
5 days ago
glaciya2018
• 0
1
vote
2
replies
123
views
Reproducibility issue with scran together with lapply and BiocParallel
scran
BiocParallel
bluster
5 days ago
ATpoint
★ 4.1k
0
votes
2
replies
200
views
featureCounts reports an error:"featureCounts: input-files.c:2890: SAM_pairer_get_next_read_BIN: Assertion `l_name < 256' failed. Aborted (core dumpe…
metaMSdata
7 days ago • updated 5 days ago
shuwangxinze1996
• 0
0
votes
8
replies
304
views
DESeq2 output used for PCA plot on R studio
DESeq2
RSTUDIO
PCAtools
updated 5 days ago by
swbarnes2
★ 1.3k • written 6 days ago by
Aaliya
• 0
0
votes
1
reply
114
views
DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR)
DESeq2
RNAseq
updated 5 days ago by
Michael Love
41k • written 5 days ago by
kcarey
• 0
1
vote
13
replies
350
views
Trying to use enrichGO
clusterProfiler
GenomicDistributionsData
KEGG
8 days ago • updated 5 days ago
fernanda.backsouza
▴ 10
2
votes
5
replies
222
views
get BM error
ensembldb
updated 5 days ago by
James W. MacDonald
65k • written 6 days ago by
Fara
• 0
0
votes
5
replies
276
views
Error for AnnotationForge makeOrgPackageFromNCBI function
OrgDb
AnnotationForge
updated 5 days ago by
James W. MacDonald
65k • written 8 days ago by
Gayatri
• 0
0
votes
0
replies
61
views
Exon-exon Junction Analysis for Variable-length Exons
Rsubread
5 days ago
Dario Strbenac
★ 1.5k
1
vote
3
replies
185
views
Different Shrunk Log2FC according with reference
DESeq2
updated 5 days ago by
Michael Love
41k • written 6 days ago by
andrebolerbarros
▴ 20
1
vote
3
replies
833
views
How to tell and visualize up/down regulation of pathways from clusterprofiler gsea output
clusterProfiler
RNASeq
updated 6 days ago by
Guangchuang Yu
★ 1.2k • written 14 months ago by
ruiqi.li
▴ 10
1
vote
4
replies
180
views
minfi::read.metharray.exp
minfiData
minfi
updated 6 days ago by
Basti
▴ 780 • written 7 days ago by
clacarion
• 0
3
votes
1
reply
276
views
Use of negative binomial model for exponential decay rate estimation using DESeq (or similar)
DESeq2
updated 6 days ago by
Michael Love
41k • written 6 days ago by
i.sudbery
▴ 40
1
vote
1
reply
144
views
Citation for edgeR user guide
edgeR
updated 6 days ago by
Gordon Smyth
50k • written 6 days ago by
k___
▴ 30
0
votes
7
replies
666
views
News:
List of Deprecated Packages for Bioc3.19
Release
Devel
ExperimentData
Software
8 weeks ago • updated 6 days ago
shepherl
3.8k
1
vote
1
reply
90
views
Cannabis OrgDb
KEGGgraph
org.Cs.eg.db
clusterProfiler
KEGG
AnnotationHubSoftware
updated 6 days ago by
James W. MacDonald
65k • written 6 days ago by
fernanda.backsouza
▴ 10
0
votes
0
replies
48
views
News:
Interested in the analysis of Spatial Omics data?
tidySpatialExperiment
SpatialExperiment
MoleculeExperiment
6 days ago
info
▴ 10
3
votes
3
replies
200
views
DESeq2 - dispersion estimation when there is none
DESeq2
updated 6 days ago by
Michael Love
41k • written 7 days ago by
WardDeb
• 0
0
votes
7
replies
275
views
Methylation Analysis experiment design using Limma
DNAMethylation
limma
methylationArrayAnalysis
12 days ago • updated 7 days ago
hortense96
• 0
5
votes
8
replies
8.8k
views
Limma design with continuous and categorical covariates
limma
batch effect
covariates
microarray
updated 7 days ago by
hortense96
• 0 • written 7.7 years ago by
dmcleanuk
▴ 30
1
vote
2
replies
958
views
DMR.Plot shows error "Error in simpleLoess(y, x, w, span, degree = degree, parametric = FALSE, : NA/NaN/Inf in foreign function call (arg 1)"
DMRcate
Gviz
updated 7 days ago by
Tim Peters
▴ 170 • written 14 months ago by
jose.ramirez1
▴ 10
0
votes
1
reply
119
views
limma-voom: Calculate the log-fold change in gene expression within a sample
limma
RNASeq
voom
updated 7 days ago by
Gordon Smyth
50k • written 7 days ago by
taur.vil
• 0
1
vote
4
replies
191
views
Error EPICv2 ChAMP
ChAMPdata
IlluminaHumanMethylationEPICv2anno.20a1.hg38
ChAMP
updated 7 days ago by
James W. MacDonald
65k • written 11 days ago by
Mirella Aurora
• 0
4
votes
6
replies
379
views
FilterByExpr low counts with small sample size
edgeR
12 days ago • updated 7 days ago
Jonathan
▴ 10
0
votes
1
reply
105
views
FDR via q-value
qvalue
updated 7 days ago by
James W. MacDonald
65k • written 10 days ago by
John
• 0
45,590 results • Page
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Replies
Comment: Assist me in determining whether the analysis process using the limma package ha
by
SSSJec
• 0
Thanks for your reply. I understand the principle of adjusted P-values, but I still want to address that issue. The description of the G…
Comment: Why p value inconsistent when using different number of pathway score?
by
Gordon Smyth
50k
Just a note that fgsea is not classical GSEA. The classical GSEA as described in the Subramanian paper and other papers from the Broad Inst…
Comment: Perform gene set enrichment analysis using fgsea on two conditions that don't ha
by
Chris
• 0
Thank you! The resource I use may mispresent the requirement input of fgsea.
Answer: Perform gene set enrichment analysis using fgsea on two conditions that don't ha
by
alserg
▴ 260
fgsea doesn't require the presence of deferentially expressed genes, it takes as the input the differential expression-based ranking of all…
Comment: Why p value inconsistent when using different number of pathway score?
by
Chris
• 0
Thanks Robert for an educative response!
Votes
Answer: Improving the design matrices vignette in RNAseq123
Answer: Perform gene set enrichment analysis using fgsea on two conditions that don't ha
Answer: Perform gene set enrichment analysis using fgsea on two conditions that don't ha
Comment: DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
Comment: Why p value inconsistent when using different number of pathway score?
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