Log In
Sign Up
about
faq
Ask a question
Latest
News
Jobs
Tutorials
Tags
Users
New Post
Latest
News
Jobs
Tutorials
Tags
Users
Log In
Sign Up
About
Limit
all time
today
this week
this month
this year
Unanswered
All posts
Sort
Update
Answers
Bookmarks
Creation
Replies
Rank
Views
Votes
0
votes
0
replies
12
views
log2FoldChange value is way too different when compared with counts(dds)
DESeq2
1 hour ago
HAK
• 0
0
votes
1
reply
77
views
How do I select a GC file (gc.wig)? (How to determine the inputdata of HMMcopy)
CopyNumberPlots
HMMcopy
CopyNumberVariation
updated 5 hours ago by
James W. MacDonald
65k • written 20 hours ago by
SSSJec
• 0
3
votes
4
replies
95
views
Help with error using camera() in limma
CAMERA
limma
8 hours ago • updated 4 hours ago
Chris
• 0
0
votes
1
reply
46
views
Fold change calculation in Diffbind vs. DESEQ2?
DiffBind
ChIPSeqData
DESeq2
updated 10 hours ago by
Michael Love
41k • written 11 hours ago by
marcusn
• 0
0
votes
1
reply
47
views
Timecourse RNASeq analysis
DESeq2
ImpulseDE2
timecour
timecoursedata
moanin
updated 10 hours ago by
Michael Love
41k • written 16 hours ago by
Aurora
• 0
0
votes
1
reply
49
views
Padj "NA" with thousands of low count although the raw read counts are quite large
DESeq2
updated 10 hours ago by
Michael Love
41k • written 1 day ago by
Agung
• 0
0
votes
0
replies
20
views
News:
BioC2024 deadlines approaching
BioC2024
Bioconductor
Community
11 hours ago
Maria Doyle
▴ 70
0
votes
1
reply
40
views
Version 3.15 S4Vectors packages are missing
S4Vectors
updated 11 hours ago by
shepherl
3.8k • written 19 hours ago by
bounlu
• 0
0
votes
1
reply
41
views
putting my own expression data in deseq2
DESeq2
updated 13 hours ago by
ATpoint
★ 4.1k • written 14 hours ago by
Dev
• 0
2
votes
2
replies
131
views
Perform gene set enrichment analysis using fgsea on two conditions that don't have differential express genes
fgsea
1 day ago
Chris
• 0
0
votes
3
replies
169
views
Assist me in determining whether the analysis process using the limma package has been executed correctly.
GEOquery
ArrayExpress
limma
DifferentialExpression
updated 7 hours ago by
James W. MacDonald
65k • written 1 day ago by
SSSJec
• 0
0
votes
0
replies
34
views
Could we detect difference at pathway level but not significant different at gene level?
GSVA
1 day ago
Chris
• 0
3
votes
3
replies
168
views
DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
DESeq2
contrasts
updated 1 day ago by
ATpoint
★ 4.1k • written 1 day ago by
bioinf
▴ 10
1
vote
1
reply
95
views
Dominance Index for Shotgun Metagenomics Data
microbiome
updated 4 days ago by
Leo Lahti
▴ 40 • written 4 days ago by
Dario Strbenac
★ 1.5k
0
votes
2
replies
161
views
PCA plot suggestions
DESeq2
PCAtools
updated 4 days ago by
Michael Love
41k • written 5 days ago by
Aaliya
• 0
1
vote
3
replies
168
views
Deseq2. decontXcounts not integers. Useful alternative?
DESeq2
celda
updated 4 days ago by
Michael Love
41k • written 5 days ago by
JalapenoCornbread
• 0
0
votes
1
reply
97
views
ATACseq merge peaks
DiffBind
updated 4 days ago by
ATpoint
★ 4.1k • written 6 days ago by
Shloka
• 0
1
vote
1
reply
138
views
Improving the design matrices vignette in RNAseq123
RNAseq123
updated 4 days ago by
Gordon Smyth
50k • written 8 days ago by
Lluís Revilla Sancho
▴ 730
1
vote
7
replies
255
views
Math expression in shapeCustom legend in EnhancedVolcano
EnhancedVolcano
5 days ago • updated 4 days ago
pl23
• 0
2
votes
9
replies
327
views
Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
DESeq2
RUVSeq
RUVseq
BatchEffect
DES
updated 5 days ago by
James W. MacDonald
65k • written 5 days ago by
Alexandre
• 0
0
votes
0
replies
64
views
One error problem occurred caused by data type during using GDCprepare to handle BRCA data
GDCRNATools
TCGA
5 days ago
glaciya2018
• 0
1
vote
2
replies
127
views
Reproducibility issue with scran together with lapply and BiocParallel
scran
BiocParallel
bluster
6 days ago • updated 5 days ago
ATpoint
★ 4.1k
0
votes
1
reply
116
views
DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR)
DESeq2
RNAseq
updated 6 days ago by
Michael Love
41k • written 6 days ago by
kcarey
• 0
0
votes
0
replies
59
views
how to do Differential splicing from SGSeq analysis
DEXSeq
SGSeq
6 days ago • updated 1 day ago
Sara
• 0
2
votes
5
replies
225
views
get BM error
ensembldb
updated 6 days ago by
James W. MacDonald
65k • written 6 days ago by
Fara
• 0
2
votes
5
replies
205
views
How to retrieve gene ontology GO class from gene list?
GO.db
goTools
updated 5 days ago by
James W. MacDonald
65k • written 7 days ago by
bandconductor
• 0
0
votes
0
replies
62
views
Exon-exon Junction Analysis for Variable-length Exons
Rsubread
6 days ago
Dario Strbenac
★ 1.5k
1
vote
3
replies
187
views
Different Shrunk Log2FC according with reference
DESeq2
updated 6 days ago by
Michael Love
41k • written 6 days ago by
andrebolerbarros
▴ 20
1
vote
4
replies
274
views
Opposite sign of LFC in count plots of DEGs (DESeq2)
DESeq2
6 days ago • updated 4 days ago
winwater0928
• 0
3
votes
1
reply
278
views
Use of negative binomial model for exponential decay rate estimation using DESeq (or similar)
DESeq2
updated 6 days ago by
Michael Love
41k • written 7 days ago by
i.sudbery
▴ 40
1
vote
1
reply
147
views
Citation for edgeR user guide
edgeR
updated 7 days ago by
Gordon Smyth
50k • written 7 days ago by
k___
▴ 30
0
votes
7
replies
668
views
News:
List of Deprecated Packages for Bioc3.19
Release
Devel
ExperimentData
Software
8 weeks ago • updated 7 days ago
shepherl
3.8k
1
vote
1
reply
94
views
Cannabis OrgDb
KEGGgraph
org.Cs.eg.db
clusterProfiler
KEGG
AnnotationHubSoftware
updated 7 days ago by
James W. MacDonald
65k • written 7 days ago by
fernanda.backsouza
▴ 10
0
votes
8
replies
309
views
DESeq2 output used for PCA plot on R studio
DESeq2
RSTUDIO
PCAtools
updated 5 days ago by
swbarnes2
★ 1.3k • written 7 days ago by
Aaliya
• 0
0
votes
0
replies
51
views
News:
Interested in the analysis of Spatial Omics data?
tidySpatialExperiment
SpatialExperiment
MoleculeExperiment
7 days ago
info
▴ 10
1
vote
4
replies
181
views
minfi::read.metharray.exp
minfiData
minfi
updated 6 days ago by
Basti
▴ 780 • written 8 days ago by
clacarion
• 0
0
votes
2
replies
209
views
featureCounts reports an error:"featureCounts: input-files.c:2890: SAM_pairer_get_next_read_BIN: Assertion `l_name < 256' failed. Aborted (core dumpe…
metaMSdata
7 days ago • updated 5 days ago
shuwangxinze1996
• 0
1
vote
2
replies
962
views
DMR.Plot shows error "Error in simpleLoess(y, x, w, span, degree = degree, parametric = FALSE, : NA/NaN/Inf in foreign function call (arg 1)"
DMRcate
Gviz
updated 7 days ago by
Tim Peters
▴ 170 • written 14 months ago by
jose.ramirez1
▴ 10
0
votes
1
reply
124
views
limma-voom: Calculate the log-fold change in gene expression within a sample
limma
RNASeq
voom
updated 8 days ago by
Gordon Smyth
50k • written 8 days ago by
taur.vil
• 0
3
votes
3
replies
202
views
DESeq2 - dispersion estimation when there is none
DESeq2
updated 7 days ago by
Michael Love
41k • written 8 days ago by
WardDeb
• 0
1
vote
4
replies
194
views
Error EPICv2 ChAMP
ChAMPdata
IlluminaHumanMethylationEPICv2anno.20a1.hg38
ChAMP
updated 8 days ago by
James W. MacDonald
65k • written 12 days ago by
Mirella Aurora
• 0
0
votes
1
reply
109
views
FDR via q-value
qvalue
updated 8 days ago by
James W. MacDonald
65k • written 11 days ago by
John
• 0
0
votes
5
replies
277
views
Error for AnnotationForge makeOrgPackageFromNCBI function
OrgDb
AnnotationForge
updated 6 days ago by
James W. MacDonald
65k • written 9 days ago by
Gayatri
• 0
0
votes
2
replies
183
views
RNAseq:Technical replicates from different batches
technicalReplicates
BatchEffect
limma
RNASeq
11 days ago • updated 8 days ago
catv
• 0
0
votes
3
replies
1.2k
views
News:
European Bioconductor Conference 2023 planning
Europe
Conference
EuroBioC
Events
News
updated 8 days ago by
lauryfriese932
• 0 • written 18 months ago by
Maria Doyle
▴ 70
6
votes
12
replies
4.8k
views
BiocFileCache/ExperimentHub/AnnotationHub temporary ERROR
BiocFileCache
AnnotationHub
ExperimentHub
6 months ago • updated 8 days ago
shepherl
3.8k
1
vote
13
replies
353
views
Trying to use enrichGO
clusterProfiler
GenomicDistributionsData
KEGG
9 days ago • updated 6 days ago
fernanda.backsouza
▴ 10
0
votes
0
replies
69
views
Unlocking Potential: NURS FPX Strategies and Insights
levi
NURSFPX
ragene20sttranscriptcluster.db
ssrch
9 days ago
vaxahey107
• 0
14
votes
17
replies
1.1k
views
Why p value inconsistent when using different number of pathway score?
limma
10 days ago • updated 2 hours ago
Chris
• 0
0
votes
0
replies
86
views
I am trying to detect alternative splicing.
ASpli
10 days ago
Sebashish
• 0
45,594 results • Page
1 of 912
Recent ...
Replies
Comment: Help with error using camera() in limma
by
Chris
• 0
Thanks James! I fixed the error. I try gene symbol instead of entrez and it worked.
Comment: limma for gene expression analysis with big and unbalanced sample size
by
XW
• 0
Could you explain more about the comment "there are no problems with using limma for unbalanced data sets" ? thanks.
Comment: Limma, eBayes different sample size of groups and adjustment
by
XW
• 0
Could you explain more about how the analysis self-correct the different sample sizes when calling DEGs in limma? Thanks
Answer: How do I select a GC file (gc.wig)? (How to determine the inputdata of HMMcopy)
by
James W. MacDonald
65k
The section of the vignette that is *directly above the one you quote* says that you can use the HMMcopy utils to do all three things. And …
Comment: Help with error using camera() in limma
by
James W. MacDonald
65k
Again, this is where you need to read the help page and try to diagnose. It says ``` index: an index vector or a list of index vectors. …
Votes
C: How to establish a subset from TxDb.Hsapiens.UCSC.hg38.knownGene DB
C: Extremely big genes in annotation TxDb packages
Make custom Txdb class objects from Txdb
Comment: Help with error using camera() in limma
Answer: Help with error using camera() in limma
Awards
• All
Popular Question
to
junhui.li11
• 0
Scholar
to
James W. MacDonald
65k
Popular Question
to
jeffrey.maurer
• 0
Centurion
to
Chris
• 0
Commentator
to
Leonard Goldstein
▴ 200
Locations
• All
WEHI, Melbourne, Australia,
just now
United States,
22 minutes ago
United States,
24 minutes ago
Switzerland,
58 minutes ago
United States,
1 hour ago
Traffic: 503 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6