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0
votes
9
replies
1.5k
views
Questions about the false positives indicated by DESeq2 and QQ plot
pre-filterin
DESeq2
updated 7 months ago by
yujin
▴ 10 • written 22 months ago by
Weiqian
▴ 10
4
votes
9
replies
448
views
Memory errors using read.bismark?
dmrseq
bsseq
29 days ago • updated 3 days ago
Juan Higareda
• 0
2
votes
9
replies
1.2k
views
Importing RSEM processed data already formatted as a summarized experiment into DESeq2
tximport
tximportData
DESeq2
10 months ago
Ndimensional
▴ 20
5
votes
9
replies
1.3k
views
Batch correction and repeated measures
RUVSeq
sva
limma
updated 22 days ago by
Gordon Smyth
50k • written 7 months ago by
Jonathan
▴ 10
1
vote
9
replies
891
views
DESeq2 not replicable results
RNASeqData
DESeq2
updated 10 months ago by
Michael Love
42k • written 10 months ago by
Matteo
• 0
4
votes
9
replies
4.4k
views
How to get DESeq2 sizefactors from data imported using tximport
deseq2
sailfish
tximport
updated 11 months ago by
IUH
• 0 • written 6.9 years ago by
stan
• 0
6
votes
9
replies
802
views
biomaRt has encountered an unknown server error.
biomaRt
host
useEnsembl
updated 3 months ago by
alexander3
• 0 • written 3 months ago by
Amparo
• 0
5
votes
9
replies
941
views
Light difference when using coef vs omit a group when compare 3 groups.
limma
4 weeks ago • updated 22 days ago
Chris
• 0
5
votes
9
replies
2.5k
views
edgeR: Should there be a normalization step for CRISPR screens? How to deal with bottlenecked screens?
edger
calcnormfactors
updated 8 months ago by
Novice_cam
• 0 • written 6.5 years ago by
knaxerova
▴ 10
0
votes
9
replies
569
views
limma fo continous variables
limma
DEGseq
updated 10 weeks ago by
James W. MacDonald
65k • written 11 weeks ago by
mia
• 0
0
votes
9
replies
3.1k
views
clarification about bumphunter value output
minfi
bumphunter
updated 11 months ago by
Jennifer Dehner
• 0 • written 6.2 years ago by
src3
• 0
2
votes
9
replies
406
views
Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
DESeq2
RUVSeq
RUVseq
BatchEffect
DES
updated 15 days ago by
James W. MacDonald
65k • written 15 days ago by
Alexandre
• 0
0
votes
9
replies
857
views
DESeq2: How to estimate effects of one factor controlling for other factors?
DESeq2
8 months ago
mjd275
• 0
2
votes
8
replies
2.4k
views
Reading HDF5 Files In The Cloud
Rhdf5lib
rhdf5
3.2 years ago • updated 8 months ago
Thomas Sandmann
▴ 90
0
votes
8
replies
719
views
Deseq2 Results are different using batch design
DESeq2
9 months ago
hemantcnaik
• 0
3
votes
8
replies
920
views
DropletUtils read10xcounts adds the 12 Cell Multiplexing Oligo tags as gene names
DropletUtils
droplet
written 5 months ago by
pcantalupo
▴ 10
0
votes
8
replies
8.6k
views
Bioconductor version cannot be validated
BiocManager
Bioconductor
updated 5 months ago by
Marcel Ramos
690 • written 2.8 years ago by
charlotte
▴ 10
0
votes
8
replies
424
views
DESeq and SVA for batch effect
DESeq2
BatchEffect
sva
9 weeks ago
michelafrancesconi8
• 0
2
votes
8
replies
1.1k
views
Failure of beta estimates to converge in DESeq2 despite noncollinearity and reasonable counts
converge
beta
DESeq2
11 months ago
Ndimensional
▴ 20
3
votes
8
replies
530
views
Downloading package mixOmics
mixOmics
11 weeks ago
noortje_bloem94
• 0
6
votes
8
replies
1.2k
views
CAGEr bug report: wrong consensus cluster results
CAGEr
updated 9 months ago by
Charles Plessy
▴ 180 • written 17 months ago by
Meng
▴ 30
1
vote
8
replies
2.8k
views
Significantly regulated genes produced by only a few affected samples
deseq2
written 6.2 years ago by
serpalma.v
▴ 60
2
votes
8
replies
2.3k
views
Error in DGEList(counts = cnt, group = group) : non-numeric values found in counts
edgeR
13 months ago • updated 11 months ago
Luis
• 0
2
votes
8
replies
1.3k
views
Reading GFF file and pre-processing, run iinfer_syntenet(), input and output
syntenet
updated 4 months ago by
Sujeevan
• 0 • written 16 months ago by
lwanderson8c
• 0
5
votes
8
replies
3.9k
views
Problem about picking soft threshold power in WGCNA software
wgcna
rnaseq
written 7.2 years ago by
panagiotis.mokos
▴ 40
3
votes
8
replies
528
views
Constrast for Time Course Experiments using Limma
limma
9 weeks ago
dl17032000
• 0
8
votes
8
replies
9.8k
views
Steps needed to change design (relevel factors) in DESeq2
deseq2
DESeq2
updated 7 months ago by
jd774
• 0 • written 4.8 years ago by
krassowski.michal
▴ 40
0
votes
8
replies
361
views
DESeq2 output used for PCA plot on R studio
DESeq2
RSTUDIO
PCAtools
updated 15 days ago by
swbarnes2
★ 1.4k • written 17 days ago by
Aaliya
• 0
0
votes
8
replies
1.1k
views
Support of parquet files for on-disk Bioconductor objects
DelayedArray
TileDBArray
8 months ago
sandmann.t
▴ 70
2
votes
8
replies
550
views
Microarray Limma Model fitting and DEG
limma
DGEanalysis
updated 6 weeks ago by
Gordon Smyth
50k • written 6 weeks ago by
Amit
• 0
1
vote
8
replies
2.6k
views
How to remove X & Y chromosome genes from RNAseq data
DESeq2
RNASeq
updated 4 months ago by
Jon
• 0 • written 3.4 years ago by
Archit
▴ 10
1
vote
8
replies
1.6k
views
Whether to build DESeq2 model with all data and then contrast groups or subset groups first, build model and then contrast?
DifferentialExpression
DESeq2
written 11 months ago by
sean.mccutcheon
• 0
10
votes
8
replies
3.9k
views
conumee error: query intensities not given for all probes.
conumee
updated 4 months ago by
Mingkee
• 0 • written 6.8 years ago by
yura.grabovska
▴ 30
0
votes
8
replies
828
views
Quickly calculating adjusted Fold Change with DESeq2
DifferentialExpression
DESeq2
6 months ago
Luca
• 0
0
votes
8
replies
1.2k
views
fail to annotate chromosome data to single cell object
error
SingleCell
annotate
updated 5 months ago by
yw24418
• 0 • written 14 months ago by
sandra.garces.19
• 0
5
votes
8
replies
1.5k
views
Can the limma package be applied to Log2 RUV-normalized data?
RUVnormalizeData
MicroarrayData
limma
updated 5 months ago by
Shaimaa Gamal
• 0 • written 11 months ago by
pg45863
▴ 10
1
vote
8
replies
554
views
edgeR error "Design matrix not of full rank"
edgeR
methylation
updated 3 months ago by
Gordon Smyth
50k • written 3 months ago by
sat
• 0
0
votes
8
replies
1.4k
views
Question about filterByExpr function edgeR
edgeR
8 months ago
lucap
• 0
4
votes
8
replies
1.2k
views
TCGAbiolinks cannot find bcr_patient_barcode
TCGAbiolinks
11 months ago
Habil Zare
▴ 200
0
votes
8
replies
1.7k
views
flowset/ncdfFlowset replace keywords doesn't work right
ncdfFlow
flowCore
updated 7 months ago by
osayou
• 0 • written 3.6 years ago by
anders.tondell
▴ 30
5
votes
8
replies
8.8k
views
Limma design with continuous and categorical covariates
limma
batch effect
covariates
microarray
updated 17 days ago by
hortense96
• 0 • written 7.7 years ago by
dmcleanuk
▴ 30
1
vote
8
replies
1.3k
views
DESeq2 test for synergistic vs additive effects
synergistic
additive
DESeq2
updated 5 weeks ago by
Wolfgang Huber
★ 13k • written 10 months ago by
Jane
▴ 10
0
votes
8
replies
717
views
GOslim missing GO term
GO.db
GOslim
5 months ago
amanda
• 0
4
votes
8
replies
2.2k
views
Can we use rlog or vst data values in limma for differential expression analysis?
DESeq2
limma
Normalization
R
updated 11 months ago by
Gordon Smyth
50k • written 17 months ago by
Sabiha
▴ 20
2
votes
8
replies
1.7k
views
Error in Create switchAnalyzeRlist
AlternativeSplicing
RNASeq
IsoformSwitchAnalyzeR
gtf
written 21 months ago by
bpshtiwan
▴ 10
0
votes
8
replies
652
views
Divergent results using exact, glmLRT, and glmQLF tests in edgeR
edgeR
12 weeks ago • updated 11 weeks ago
Marianna
▴ 20
4
votes
7
replies
2.1k
views
should statmod be a dependency of limma and not a suggests?
limma
updated 9 months ago by
Gordon Smyth
50k • written 7.9 years ago by
nathan
• 0
1
vote
7
replies
872
views
Permutation test in DESeq2?
DESeq2
updated 7 months ago by
Michael Love
42k • written 7 months ago by
michellewei
• 0
3
votes
7
replies
892
views
DESeq2 alternative hypyothesis: testing for genes NOT significantly downregulated
DESeq2
8 months ago
casco
▴ 10
8
votes
7
replies
1.2k
views
Why is DMRcate not available for Bioconductor version 3.18?
DMRcate
written 5 months ago by
Daniel E. Weeks
▴ 30
1,742 results • Page
3 of 35
Recent ...
Replies
Comment: Can't post a question containing code?
by
NATASHA
• 0
did you find a solution? I´m having the same problem
Comment: error
by
NATASHA
• 0
Please ignore, i didnt copy all the scrip
Comment: Extremely small p-values using Limma for proteomic data
by
James W. MacDonald
65k
If you ever find yourself using the `@` function, you should reconsider what you are doing. There are vanishingly small instances when an e…
Comment: How to use bootRanges to bootstrap small RNA loci (nullranges package)
by
Michael Love
42k
Here is some example code <https://gist.github.com/mikelove/cbbc84634b6854c865cb5eb08ad9b245>
Comment: How to use spike-in information (sequences from another species) with DESeq2::DE
by
maria.soler
• 0
Hi, I have a question barely related to this topic. Do you use a custom reference genome combining your organism and the one used as a spik…
Votes
A: AIC-like measure for DESeq2
Comment: Extremely small p-values using Limma for proteomic data
Comment: Log-cpm values from limma
Comment: Log-cpm values from limma
Comment: deseq2 results
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