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RNA-Seq
•
reset
3
votes
4
replies
1.9k
views
Pipeline for an Anova-like analysis in edgeR
edgeR
anova
rna-seq
4.5 years ago • updated 4.4 years ago
Antonio M.
• 0
1
vote
2
replies
1.6k
views
multifactorial design matrix
designmatrix
RNA-seq
limma
updated 4.5 years ago by
Gordon Smyth
53k • written 4.5 years ago by
athief
▴ 20
1
vote
7
replies
2.0k
views
how to make a contrast of uniquely DE genes
edgeR
limma
RNA-seq
DEGseq
4.5 years ago
decppced
• 0
1
vote
3
replies
1.2k
views
Questions regarding DESeq2 experimental setup and lfcShrink
DESeq2
RNA-seq
updated 4.6 years ago by
Michael Love
43k • written 4.6 years ago by
zroger499
• 0
0
votes
0
replies
1.2k
views
Normalization for WGCNA on two organisms (virus-host)
RNA-Seq
DESeq2
WGCNA
Normalization
4.7 years ago
wrzl
• 0
3
votes
9
replies
3.2k
views
summarizeToGene from tximeta: not all 'rnames' found or unique.
RNA-seq
tximeta
4.8 years ago
Pufall, Miles A
▴ 30
0
votes
1
reply
1.4k
views
Limma/Voom Normalized Counts
limma
RNA-seq
updated 4.8 years ago by
Gordon Smyth
53k • written 4.8 years ago by
jackiesalzbank
• 0
0
votes
2
replies
2.4k
views
RNA-seq read simulation for benchmarking differential expression/splicing variance
polyester
simulation
RNA-seq
syntheticdata
updated 4.9 years ago by
Gordon Smyth
53k • written 4.9 years ago by
Endre Sebestyén
▴ 70
0
votes
2
replies
1.7k
views
Pathway analysis using GAGE returns all NA values
gage
rna-seq
pathwayanalysis
updated 2.4 years ago by
Rob
• 0 • written 4.9 years ago by
microPhD
• 0
3
votes
6
replies
4.1k
views
DESeq2: When to split multiple group samples for 'more' accurate sizefactors, dispersions etc.
Dispersion
RNA-seq
DESeq2
Multiple-group
updated 4.6 years ago by
Michael Love
43k • written 4.9 years ago by
Kelen
▴ 10
0
votes
3
replies
2.3k
views
GSVA on gene sets with 2 genes
GSVA
gene sets
RNA-seq
updated 4.9 years ago by
Robert Castelo
★ 3.4k • written 5.0 years ago by
Endre Sebestyén
▴ 70
0
votes
3
replies
1.6k
views
Weird set of samples in plant gene atlas study that appear over-corrected after DESeq2 normalization
RNA-Seq
DESeq2
updated 4.9 years ago by
Michael Love
43k • written 4.9 years ago by
julie.green
• 0
0
votes
0
replies
1.7k
views
how to find consensus modules between 2 datasets by different platform through WGCNA
consensusmodule
microarray
RNA-seq
WGCNA
5.0 years ago • updated 4.9 years ago
modarzi
▴ 10
0
votes
0
replies
1.4k
views
Job:
Bioinformatics Developer / Computational Biologist
bioinformatics
rna-seq
shiny apps
R
algorithm development
Job
5.0 years ago
mmartin
▴ 20
0
votes
0
replies
1.1k
views
circRNAprofiler - miRNA binding sites
circRNA
RNA-seq
circRNAprofiler
5.0 years ago
Terra
• 0
0
votes
0
replies
1.9k
views
HISAT2/StringTie/Ballgown for Novel Transcript Isoform Differential Expression
isoform
RNA-seq
hisat2
stringtie
ballgown
5.0 years ago
ra711
• 0
3
votes
3
replies
1.5k
views
Problem to understand Interactions at any time
RNA-seq
edgeR
interactions
updated 5.0 years ago by
Gordon Smyth
53k • written 5.0 years ago by
vm.higareda
▴ 10
0
votes
3
replies
1.8k
views
Pathway analysis of bacterial RNA-seq data
annotation
rna-seq
pathway analysis
5.0 years ago
microPhD
• 0
5
votes
6
replies
2.3k
views
RUVseq to remove a library size artefact in a zebrafish brain RNA-seq dataset
RUVSeq
RUV
RNA-seq
updated 5.0 years ago by
James W. MacDonald
68k • written 5.0 years ago by
karissa.barthelson
▴ 20
3
votes
1
reply
1.2k
views
How do you create a targets dataframe in edgeR?
edgeR
RNA-SEQ
updated 5.0 years ago by
James W. MacDonald
68k • written 5.0 years ago by
Terra
• 0
1
vote
1
reply
1.6k
views
Having issues mapping gene Ids during ReportingTools::publish() from Bioconductor.
ReportingTools
RNA-Seq
RMarkdown
updated 5.1 years ago by
James W. MacDonald
68k • written 5.1 years ago by
anthony.nash
▴ 20
1
vote
4
replies
2.5k
views
Issues with DESeq2 LRT for time-course experiment
rna-seq
R
DESeq2
LRT
updated 5.1 years ago by
Kevin Blighe
★ 4.0k • written 5.1 years ago by
luke.briody
▴ 10
0
votes
1
reply
1.3k
views
dupRadar: low % of duplicates for 150bp PE RNAseq
dupRadar
150bp
RNA-seq
updated 5.1 years ago by
sergisayolspuig
▴ 80 • written 5.1 years ago by
olegderyagin
• 0
1
vote
4
replies
2.5k
views
Using different LFC shrinkage estimators for spike-in normalised RNA-seq data
deseq2
apeglm
rna-seq
spike-in
updated 5.1 years ago by
Michael Love
43k • written 5.1 years ago by
nadezda.fursova
▴ 10
0
votes
0
replies
1.7k
views
Job:
Scientist 1, Bioinformatics (Research | Berkeley, CA | Full Time)
Bioinformatics
drug-discovery
RNA-seq
CRISPR
Job
5.1 years ago
yzhang
• 0
0
votes
0
replies
1.3k
views
Job:
Postdoctoral Fellow in Sars-CoV-2 genomics and transcriptomics @ Paul-Ehrlich-Institute, Germany
rna-seq
phylogenetics
protein modelling
single-cell sequencing
Job
5.2 years ago
liam.childs
• 0
3
votes
2
replies
6.6k
views
DESeq2 design formula with multiple factors
deseq2
RNA-Seq
updated 5.2 years ago by
Michael Love
43k • written 5.2 years ago by
Helena
▴ 10
0
votes
2
replies
3.8k
views
cmdscale() error: NA values not allowed in 'd', ask for help.
cmdscale
zinbwave
scRNA-seq
single cell sequencing
RNA-seq
updated 5.2 years ago by
davide risso
▴ 980 • written 5.3 years ago by
Wagaa
▴ 10
7
votes
6
replies
1.5k
views
Odd DESeq2 results - high significance in genes with too many zeros
deseq2
rna-seq
updated 5.2 years ago by
Michael Love
43k • written 5.2 years ago by
mbk0asis
• 0
1
vote
2
replies
1.2k
views
EdgeR blocking factor - missing paired sample
EdgeR
edgeR
RNA-seq
differential expression analysis
blocking factors
updated 5.3 years ago by
Gordon Smyth
53k • written 5.3 years ago by
Lucy
▴ 60
0
votes
2
replies
2.2k
views
DESeq2 doubts about transformations + nbinomWaldtest warning
rna-seq
deseq2
warnings
nbinomWaldTest
transformation
updated 5.3 years ago by
Michael Love
43k • written 5.3 years ago by
andreia
▴ 10
1
vote
0
replies
1.3k
views
Pathview : why are some genes not colored?
pathview
rna-seq
5.3 years ago
okna0215
▴ 10
1
vote
0
replies
1.5k
views
Job:
Bioinformatics Data Analyst
R
RNA-seq
job
Job
5.3 years ago
António Miguel de Jesus Domingues
▴ 510
1
vote
4
replies
2.4k
views
How to compare a single RNA-Seq sample with microarray samples?
voom
rna-seq
microarray
updated 5.3 years ago by
James W. MacDonald
68k • written 5.3 years ago by
Sam
▴ 10
4
votes
8
replies
3.3k
views
Don't let TopGo to trimm long GO names
go
software error
topgo
rna-seq
updated 2.7 years ago by
James W. MacDonald
68k • written 5.3 years ago by
morteza.aslanzadeh
▴ 30
0
votes
2
replies
3.5k
views
Normalisation of rlog and VSD transformation
deseq2
rna-seq
R
updated 5.4 years ago by
Kevin Blighe
★ 4.0k • written 5.4 years ago by
thjnant
▴ 10
3
votes
8
replies
4.5k
views
Normalisation in DESeq2
deseq2
rna-seq
updated 5.4 years ago by
Kevin Blighe
★ 4.0k • written 5.4 years ago by
thjnant
▴ 10
1
vote
3
replies
2.6k
views
Pairwise comparison of multiple groups with different base
deseq2
rna-seq
r
updated 5.4 years ago by
Michael Love
43k • written 5.4 years ago by
thjnant
▴ 10
4
votes
6
replies
5.5k
views
linear mixed models using edgeR+limma
linear mixed model
edger
limma
voom
RNA-seq
updated 5.4 years ago by
Gordon Smyth
53k • written 5.4 years ago by
weichengz
▴ 10
1
vote
7
replies
3.7k
views
High padj in DESeq2
deseq2
RNA-seq
microRNA
updated 5.4 years ago by
Kevin Blighe
★ 4.0k • written 5.4 years ago by
saulwang0102
▴ 10
2
votes
2
replies
2.2k
views
subsetting VST counts from one dataset
deseq2
rna-seq
updated 5.4 years ago by
Michael Love
43k • written 5.4 years ago by
A
▴ 60
0
votes
1
reply
788
views
Where to file data for RNA-seq workflow: gene-level exploratory analysis and differential expression?
rna-seq
updated 5.4 years ago by
Kevin Blighe
★ 4.0k • written 5.5 years ago by
anamarija
▴ 10
3
votes
4
replies
2.1k
views
Massive L2FC in DESeq2
deseq2
ashr
rna-seq
5.4 years ago
Alex
• 0
1
vote
1
reply
2.1k
views
EDASeq gene length normalization
edaseq
r
rna-seq
updated 5.4 years ago by
Kevin Blighe
★ 4.0k • written 5.4 years ago by
karlaarz
• 0
0
votes
1
reply
748
views
Having issues generating manhattan plot in deseq2
deseq2
RNA-seq
updated 5.5 years ago by
Michael Love
43k • written 5.5 years ago by
adeler001
• 0
0
votes
0
replies
1.1k
views
Export Results from BatchQC Shiny App
BatchQC
RNA-seq
R
Shiny
batch-effect
5.5 years ago
manninm
• 0
0
votes
5
replies
3.4k
views
Using raw pvalue instead of adjusted pvalue in Deseq 2
DESeq2
RNA-seq
updated 5.5 years ago by
Michael Love
43k • written 5.5 years ago by
adeler001
• 0
2
votes
4
replies
3.0k
views
DESeq2 Error: package or namespace load failed for 'DESeq2'
RNA-Seq
DESeq2
updated 5.5 years ago by
Istvan Albert
▴ 50 • written 5.5 years ago by
DataFanatic
▴ 10
2
votes
1
reply
1.5k
views
batchelor batch correction correction runs into errors
batchelor
batch-correction
mnncorrect
fastmnn
rna-seq
updated 5.5 years ago by
Aaron Lun
★ 29k • written 6.1 years ago by
firestar
▴ 20
4
votes
3
replies
1.5k
views
Rsamtools summarizeOverlaps produces low counts
Rsamtools
summarizeOverlaps
RNA-seq
updated 5.5 years ago by
James W. MacDonald
68k • written 5.5 years ago by
liorglic
▴ 10
418 results • Page
2 of 9
Recent ...
Replies
Answer: How to model DE for RNA-seq in concordant monozygotic twins?
by
Gordon Smyth
53k
Your question is more about science than it is about software syntax, and I can't give advice about how to conduct a scientific analysis, n…
Comment: incomplete imputation of missing values with LEA impute() function
by
Gordon Smyth
53k
Nadia, please tag your question with the name of the package ("LEA") that you are asking about. "impute" is the name of a different Biocond…
Comment: Sharing a collection of datasets in ExperimentHub
by
Aaron Lun
★ 29k
FYI the **scRNAseq** packages uses a non-ExperimentHub storage platform, see [here](https://github.com/ArtifactDB/gypsum-worker) for more d…
Comment: Error Loading Experiment from SimBenchData Package
by
ATpoint
★ 4.9k
Is the Seurat package installed? The error somewhat suggests that it could be related, and at https://www.bioconductor.org/packages/release…
Answer: Help with defining groups
by
Gordon Smyth
53k
Defining sample groups is separate to reading in the DIA-NN data, because the sample conditions and covariates are not necessarily coded in…
Votes
Comment: incomplete imputation of missing values with LEA impute() function
Answer: Expected memory usage for analyzing large single-cell seq datasets (> 450,000 ce
Answer: ordinary t from limma [was: How would I normally compare swirl?]
Comment: readDNAStringSet with non DNA characters
Comment: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
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