Showing : rna • reset
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5
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448
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Help with DESeq results to pathways with the fgsea package
fgsea rna
updated 10 weeks ago by 65k • written 11 weeks ago by • 0
0
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1
reply
264
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Running Deseq2 with all samples vs samples for each comparison separately
RNA DESeq2
updated 3 months ago by 41k • written 3 months ago by • 0
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2
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1.0k
views
Access Ensembl GRCH38 Version 87 Using BiomaRt
biomaRt ensembldb RNA ensembl
updated 12 months ago by ★ 2.0k • written 12 months ago by • 0
3
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6
replies
1.9k
views
Limma: log2 of TMM values for RNA-Seq Analysis and PlotMDS()?
Log2 edgeR RNA limma Normalization
17 months ago Sara • 0
0
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0
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522
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Correct GFF3 translation for FeatureCounts
RNASeq FeatureCounts Rna
18 months ago rridley3 • 0
1
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1
reply
693
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Confused about design for RNA seq experiment
RNAseq ExperimentData RNAseq123 RNA ExperimentalDesign
updated 19 months ago by ★ 4.0k • written 19 months ago by ▴ 30
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763
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Job: Scientist/Senior Scientist – Bioinformatics / Computational Biology
MassSpectrometryData RNA DifferentialExpression
20 months ago António Miguel de Jesus Domingues ▴ 490
0
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2
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826
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Rsubread align --R session aborts
RNA Alignment Rsubread
20 months ago sreekalasn • 0
2
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1
reply
1000
views
Error in RNAseq123 analysis, unexpected symbol.
RNAseq123 Rna
updated 22 months ago by 50k • written 22 months ago by • 0
1
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2
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1.6k
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Is it necessary to standardize Combat in R?
RNA Combat sva R
23 months ago takemoto • 0
1
vote
1
reply
775
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Description of the headers obtained from the Eligos2 output
methylation RNA native epitranscriptomics
updated 2.1 years ago by ▴ 90 • written 2.1 years ago by ▴ 10
0
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1
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858
views
Ensembl dataset version
tximport rna rnaseqGene biomaRt
updated 2.3 years ago by ★ 4.0k • written 2.3 years ago by • 0
0
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0
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584
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Cross-cultivar RNA-seq analysis
RNA Normalization
3.0 years ago nancy • 0
0
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4
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1.3k
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Fitting a continuous variable with batch effect using Limma. Also a question about normalization.
limma edgeR rna
3.0 years ago Nirad ▴ 10
0
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3
replies
2.2k
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Pearson correlation between gene expression and phenotype
edgeR RNASeq
updated 3.4 years ago by 50k • written 3.4 years ago by ▴ 10
4
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3
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2.2k
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How to resolve NAs when annotating a diff gene list with org.Hs.eg.db terms?
annotation rna
updated 3.5 years ago by 65k • written 3.5 years ago by ▴ 20
2
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7
replies
1.2k
views
Affycoretools package, HTA 2.0 annotation for non-coding RNA
affy rna hta2.0 r
updated 3.9 years ago by 65k • written 3.9 years ago by • 0
1
vote
1
reply
777
views
How to interpret coefficients of time course modeled data with DESeq2?
deseq2 RNA
updated 4.0 years ago by 41k • written 4.0 years ago by • 0
0
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6
replies
1.2k
views
Bizarre Dispersion Plots
deseq2 RNA RNASeq
updated 4.1 years ago by 41k • written 4.1 years ago by • 0
0
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0
replies
808
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Job: Post-doctoral Fellowship at BC Cancer Research Center, Vancouver BC
single cell RNA Data analysis Bioinformatics Job
4.8 years ago jmaanaki • 0
1
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1
reply
1.3k
views
how to filter genes in significant module?
RNA WGCNA filter
4.8 years ago modarzi ▴ 10
0
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2
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878
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DESeq and checkFullRank(modelMatrix)
RNA DESeq2
updated 5.0 years ago by 41k • written 5.0 years ago by ▴ 10
2
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1
reply
813
views
DESeq2 statistic and design
deseq2 RNA
updated 5.0 years ago by 41k • written 5.0 years ago by ▴ 10
5
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5
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1.4k
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Association analysis between gene expression and Phenotype
rna singlecell limma
updated 5.1 years ago by ★ 28k • written 5.1 years ago by ▴ 20
2
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3
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1.1k
views
Number and percent of mapped reads from RNA seq analysis.
rna-seq rna
updated 5.1 years ago by ★ 13k • written 5.1 years ago by ▴ 20
1
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0
replies
931
views
Job: 1 Computational Biologist and 1 Molecular Biologist
computational biology molecular biology RNA r-loop ncRNA Job
5.5 years ago l.brown ▴ 10
0
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0
replies
930
views
R code for finding wobble/redundant positions in an RNA string
r code rna wobble
6.2 years ago fawazfebin ▴ 60
0
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1
reply
978
views
Time series comparison with DESEQ2
RNA rnaseq deseq2
updated 6.7 years ago by 41k • written 6.7 years ago by ▴ 20
0
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1
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1.4k
views
RNA sequencing analysis for time point experiment using DeSeq2
deseq2 RNA rnaseq
updated 6.7 years ago by 41k • written 6.7 years ago by ▴ 20
1
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1
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1.3k
views
Outliers - to remove or to not remove that is the question
outliers rna microarray
updated 6.9 years ago by ▴ 420 • written 6.9 years ago by ▴ 30
5
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4
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1.3k
views
Experiment design contrasts any time edgeR
DGE rnaseq RNA edger
updated 7.0 years ago by • 0 • written 7.0 years ago by ▴ 40
3
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6
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1.9k
views
Regressing effect of treatment on RNA-seq expected count from rsem.
RNA rna-seq science bioinformatics
updated 7.0 years ago by 65k • written 7.0 years ago by ▴ 20
6
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5
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3.8k
views
Clustering differential expression results from voomWithQualityWeights
RNA limma rsem edgeR
updated 7.0 years ago by 50k • written 7.0 years ago by ▴ 20
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0
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1.5k
views
Finding candidate binding sites for RNA binding proteins in R
R bioconductor RNA mRNA RNA binding proteins
7.1 years ago Walter F. Baumann ▴ 10
3
votes
1
reply
1.6k
views
Targeted RNASeq/CaptureSeq DE Analysis
EdgeR deseq2 RNA differential expression
updated 7.6 years ago by ★ 28k • written 7.6 years ago by • 0
0
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4
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5.0k
views
RNAseq Adjusting for Covariates?
RNA rnaseq Covariate adjustment deseq
updated 8.2 years ago by 41k • written 8.2 years ago by • 0
0
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2
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1.3k
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Normalizzation indipentent data from RNAAeq experiment
rna normalization
9.6 years ago aristotele_m ▴ 40
37 results • Page 1 of 1
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Replies
Answer: DESEQ2 IHW and Apelgm method for Shrinkage (adding s values to FDR) by Michael Love 41k
> I got many p values that had "1.000000e+00" and padj "1" and stat of "0" when I added the Log threshold of LFC >1 and LFC < -1...is this …
Comment: Trying to use enrichGO by fernanda.backsouza ▴ 10
With all my love, thank you Guido, GOxploreR beeing a big ally for me. I don't know how to be grateful right now.
Comment: DESeq2 output used for PCA plot on R studio by swbarnes2 ★ 1.3k
You didn't generate it with an experiment, you made it up: Your PCA doesn't look like a good RNASeq experiment, because it's not.
Answer: Opposite sign of LFC in count plots of DEGs (DESeq2) by swbarnes2 ★ 1.3k
Your contrast is comparing LGR5 to Homeostasis. The bottom 2 are fine, it's the top one that is wrong. Are you sure this step isn't misla…
Comment: get BM error by James W. MacDonald 65k
I believe you need NCBI Gene IDs for KEGG, in which case you may need to map. The three genes you have shown here don't map, and of those t…
Votes
C: when to apply quantile normalization with voom in limma/voom framework with RNA-
A: How to know if I should use voomWithQualityWeights() or not?
A: Weird results (ribosomal proteins / Y-linked genes) in limma/voom
Answer: Log-cpm values from limma
Answer: Log-cpm values from limma
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