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35
views
Limma for paired and unpaired samples. Strange number of DEGs in unpaired samples
limma
lmfit
R
DifferentialExpression
updated 4 hours ago by
ATpoint
★ 1.2k • written 5 hours ago by
Dimitris
• 0
0
votes
0
replies
18
views
Job:
Bioconductor Community Manager
Community
Teaching
Bioconductor
11 hours ago
Matthew Ritchie
▴ 1000
0
votes
0
replies
23
views
Package name already exists due to previous issue
Bioconductor
16 hours ago
Daan
• 0
0
votes
0
replies
32
views
Diffbind: different results on the same sample depending on contrast parameters
DifferentialExpression
DiffBind
17 hours ago
Luca
• 0
1
vote
4
replies
70
views
How to figure out the FC value from Limma
limma
DEGseq
DifferentialExpression
updated 1 hour ago by
Kevin Blighe
3.5k • written 18 hours ago by
beslinail
• 0
3
votes
3
replies
90
views
Question on RNA-Seq single sample data analysis in EdgeR
R
edgeR
limma
RNASeq
Normalization
updated 6 hours ago by
Gordon Smyth
45k • written 1 day ago by
mohammedtoufiq91
▴ 10
0
votes
6
replies
102
views
regression analysis for epic array methylation data using limma
limma
Epigenetics
STATISTICS
R
1 day ago • updated 2 hours ago
nabiyogesh
▴ 10
0
votes
1
reply
41
views
scRNASeq reference at a transcript level
EWCE
scRNAseq
updated 1 day ago by
Basti
▴ 280 • written 1 day ago by
marc.tormo
• 0
2
votes
2
replies
48
views
How to run GSVA with partially NA genes
GSVA
1 day ago • updated 10 hours ago
blakebowen
• 0
0
votes
0
replies
25
views
error with: .make_splicings <- function(exons, cds, stop_codons=NULL)
GenomicFeatures
updated 23 hours ago by
James W. MacDonald
59k • written 1 day ago by
rsnorman
• 0
0
votes
2
replies
82
views
Using limma to read Illumina HT-12 v4 data
limma
updated 2 days ago by
Gordon Smyth
45k • written 2 days ago by
orbitofrontal
• 0
2
votes
6
replies
144
views
Warnings when using "ashr" in lfcshrinkage for DESeq2
RNASeq
DESeq2
2 days ago • updated 1 day ago
andrebolerbarros
▴ 10
0
votes
0
replies
38
views
CPM after RUVseq
RUVSeq
CPM
Normalization
2 days ago
Jess
• 0
0
votes
2
replies
62
views
R: problem with running time-series analysis software: Mfuzz
time-series
mfuzz
replace=FALSE
3 days ago
soda
▴ 80
2
votes
4
replies
96
views
Robust Multi-array Average could get negative value or not?
pd.hg.u219
oligo
AffymetrixChip
3 days ago
Yang Shi
• 0
2
votes
2
replies
74
views
how to make a group to apply the data to CAMERA
CAMERA
4 days ago • updated 2 days ago
Akira
• 0
1
vote
5
replies
129
views
UniprotKB AC/ID to Entrez ID Conversion
ID
Uniprot
EntrezID
4 days ago • updated 1 day ago
tom.kloter
• 0
0
votes
1
reply
90
views
Function Request for Rsubread or tximport: parsing command-line featureCounts output
tximport
Rsubread
updated 4 days ago by
ATpoint
★ 1.2k • written 4 days ago by
johnmcma
▴ 10
0
votes
4
replies
123
views
performing the differential binding in ChIP-seq
ChIP-seq
updated 2 days ago by
ATpoint
★ 1.2k • written 4 days ago by
Bogdan
▴ 640
3
votes
5
replies
125
views
Print EnhancedVolcano Plot as jpeg or similar in R Notebook
EnhancedVolcano
updated 4 days ago by
Kevin Blighe
3.5k • written 5 days ago by
Bine
▴ 20
1
vote
1
reply
68
views
Is it necessary to standardize Combat in R?
RNA
Combat
sva
R
5 days ago • updated 4 days ago
takemoto
• 0
4
votes
10
replies
207
views
Methods for removing unexpressed probes of Affymetrix Human Genome U219 Array
pd.hg.u219
Microarray
oligo
AffymetrixChip
5 days ago • updated 4 days ago
Yang Shi
• 0
0
votes
0
replies
33
views
Regarding installing RAMA, BRIDGE, and iterative BMA packages in MultiExperiment Viewer (MeV).
MeV+R
MeV
5 days ago
Souvik
• 0
1
vote
4
replies
108
views
ashr vs apeglm for interaction data
lfcShrink
DESeq2
5 days ago • updated 15 hours ago
Ali Barry
▴ 30
0
votes
1
reply
55
views
after calcNormFactors with EdgeR, how to get TMM normalized matrix?
Normalization
edgeR
updated 5 days ago by
Gordon Smyth
45k • written 5 days ago by
kevin
• 0
3
votes
4
replies
123
views
Quantile Normalization after rma or not?
limma
Microarray
oligo
5 days ago • updated 4 days ago
Yang Shi
• 0
1
vote
4
replies
202
views
different results in DESeq2 when comparing groups in two ways
DESeq2
5 days ago • updated 4 days ago
zohreh.fazelan
• 0
1
vote
1
reply
68
views
Using featureCounts and downloading Rsubread
featureCounts
RNASeq
Rsubread
R
updated 3 days ago by
Gordon Smyth
45k • written 5 days ago by
margo
• 0
2
votes
1
reply
61
views
Get MANE transcripts from TxDb.Hsapiens.UCSC.hg38.knownGene
AnnotationDbi
updated 5 days ago by
James W. MacDonald
59k • written 6 days ago by
nhaus
• 0
0
votes
1
reply
86
views
ChIP-seq peak annotation to gene in ChIPseeker
ChIPseeker
6 days ago • updated 3 days ago
Gyan Prakash Mishra
• 0
0
votes
1
reply
55
views
champ.SVD() error, assumes standard class() function will return single string
ChAMP
updated 6 days ago by
Basti
▴ 280 • written 6 days ago by
ramonfallon
• 0
4
votes
6
replies
177
views
How to best visualize multi-level transcriptomics experiment data?
ggplot2
Transcriptomics
Visualization
R
limma
6 days ago • updated 1 day ago
mohammedtoufiq91
▴ 10
3
votes
3
replies
114
views
edgeR: the F-statistics changed after removing lowly expressed gene
glmQLFTest
edgeR
updated 6 days ago by
Gordon Smyth
45k • written 6 days ago by
Pei
• 0
0
votes
0
replies
30
views
Rcpp std::chdir
Rcpp
6 days ago
ullrich
▴ 10
0
votes
1
reply
61
views
DESeq2 paired design: Differential list comparing fold changes
DESeq2
updated 6 days ago by
swbarnes2
★ 1.0k • written 6 days ago by
athira
• 0
0
votes
0
replies
37
views
RPX PXDataset error
PRIDE
rpx
PXDataset
6 days ago
gianluigi.caltabiano
• 0
2
votes
1
reply
66
views
Difference between raw counts and featureCounts for DESeq2
deseq
featurecounts
counts
DESeq2
updated 6 days ago by
swbarnes2
★ 1.0k • written 6 days ago by
Hicham
• 0
0
votes
3
replies
105
views
DiffBind: 0 consensus peaks for dba.peakset: New bug / undocumented change introduced between version 2.14 and 3.4.11
DiffBind
updated 6 days ago by
Rory Stark
★ 4.4k • written 7 days ago by
chrarnold
• 0
2
votes
2
replies
80
views
Methods for removing unexpressed probes of Human Transcriptome Array HTA 2.0
hta20transcriptcluster.db
Microarray
oligo
7 days ago
Yang Shi
• 0
0
votes
0
replies
50
views
LEA get Q-matrix from best snmf() run
LEA
8 days ago
janis
• 0
0
votes
1
reply
91
views
The results(dds) function in DESeq2 gives Na values for padj
DESeq2
updated 8 days ago by
ATpoint
★ 1.2k • written 8 days ago by
keshav.prasad.gubbi
• 0
0
votes
4
replies
134
views
featureCounts: 0% successfully assigned fragments
featureCounts
8 days ago • updated 5 days ago
KMS
• 0
0
votes
1
reply
57
views
AssignChromosomeRegion not working in Rstudio 4.2
ChIPpeakAnno
ChIPSeq
updated 7 days ago by
Ou, Jianhong
★ 1.3k • written 8 days ago by
Abhilansh
• 0
2
votes
2
replies
82
views
rtracklayer S.h
install
8 days ago • updated 6 days ago
gibran
• 0
2
votes
2
replies
101
views
Handling NA values in limma's voom design matrix
limma
voom
design
NAs
updated 9 days ago by
Gordon Smyth
45k • written 9 days ago by
dr
▴ 10
0
votes
13
replies
304
views
EnrichGO not working with db created from annotationhub
clusterProfiler
AnnotationHubData
GO
updated 6 days ago by
Guido Hooiveld
★ 3.2k • written 9 days ago by
Lucía
• 0
0
votes
1
reply
81
views
miRNA analysis with DESeq2
mirna
DESeq2
updated 10 days ago by
Michael Love
37k • written 10 days ago by
Faith.omonijo
• 0
0
votes
4
replies
161
views
different sample size from each individual
DESeq2
updated 10 days ago by
Michael Love
37k • written 10 days ago by
LW
• 0
0
votes
1
reply
89
views
resultsNames(dds) is not similar to coldata(dds)?
DESeq2
10 days ago
A.J.
▴ 20
0
votes
2
replies
72
views
toGRanges help!
GenomicRanges
toGRanges
Bioconductor
10 days ago
samantha.haque
• 0
1,000 results • Page
1 of 20
Recent ...
Replies
Comment: ROC plot for OPLS-DA
by
etienne.thevenot
▴ 20
I would suggest to concatenate your training and testing data rowwise, and to apply the `opls` method with the subset parameter to select t…
Comment: regression analysis for epic array methylation data using limma
by
nabiyogesh
▴ 10
Dear Basti, thank you so much! Could you please also help me how to arrange samples in the same order in rows (targests2) and columns in (…
Comment: How to figure out the FC value from Limma
by
ATpoint
★ 1.2k
You probably have opened that file in Excel before. Get it again directly from GEO2R, or even better take the R script GEO2R offers and run…
Comment: regression analysis for epic array methylation data using limma
by
Basti
▴ 280
The columns of your design matrix `var` corresponds to the coefficients that are fitted by limma. If you check your design matrix, you will…
Comment: How to figure out the FC value from Limma
by
beslinail
• 0
I tried to FC column to dbl from chr. But FC colomn is changed by NA genlist_filter <- read_csv(file = "AD_control5.csv") gen…
Votes
A: Can we use glmQLFit instead of glmFit and then glmLRT in edgeR
A: Can we use glmQLFit instead of glmFit and then glmLRT in edgeR
Answer: Limma for paired and unpaired samples. Strange number of DEGs in unpaired sample
A: Very low EdgeR Pvalues with very small sample size
A: Very low EdgeR Pvalues with very small sample size
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