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25
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Question about collapsing ASVs by genus level during DESeq2 analysis
Bioconductor
1 day ago
acharyapratiksha24
• 0
0
votes
1
reply
83
views
NA values for DESeq2 p-values and adjusted p-values using large sample sizes
Bioconductor
updated 5 days ago by
ATpoint
★ 5.0k • written 6 days ago by
Gabriel
• 0
0
votes
1
reply
61
views
Gatins Set error
flowCore
Bioconductor
updated 6 days ago by
James W. MacDonald
68k • written 6 days ago by
yoav.lubelsky
• 0
0
votes
0
replies
34
views
Job:
Senior Teaching Fellow (MSc Computational Biomedicine)
Bioconductor
6 days ago
s.samarajiwa
• 0
0
votes
0
replies
32
views
What R/Bioconductor tools would you recommend for the analysis of sncRNA, specifically piRNA?
Bioconductor
6 days ago
Matthew Thornton
▴ 380
0
votes
1
reply
84
views
How to model DE for RNA-seq in concordant monozygotic twins?
RNASeq
variancePartition
RUVSeq
limma
edgeR
updated 2 days ago by
Gordon Smyth
53k • written 7 days ago by
ilaria.massaiu
• 0
0
votes
0
replies
67
views
News:
Bioconductor 3.22 Release Schedule
Devel
Release
Bioconductor
24 days ago • updated 7 days ago
shepherl
4.2k
1
vote
1
reply
99
views
incomplete imputation of missing values with LEA impute() function
Bioconductor
LEA
8 days ago • updated 7 days ago
nadia
• 0
0
votes
1
reply
139
views
Streamlining the computing time for MiloDE p-value correction in large dataset?
SingleCellExperiment
edgeR
miloR
Bioconductor
updated 5 days ago by
ATpoint
★ 5.0k • written 9 days ago by
Joseph
• 0
0
votes
0
replies
39
views
featureCounts -L: high unassigned:mapq fraction
Bioconductor
updated 8 days ago by
James W. MacDonald
68k • written 9 days ago by
sikora
• 0
1
vote
11
replies
6.7k
views
DiffBind - question on the use of filtered or unfiltered input data
DiffBind
written 2.5 years ago by
Oscar
▴ 10
0
votes
2
replies
191
views
Help with defining groups
limpa
12 days ago • updated 1 day ago
Phinney, Brett
▴ 10
1
vote
2
replies
142
views
Error Loading Experiment from SimBenchData Package
Bioconductor
12 days ago • updated 2 days ago
ksankaran
• 0
0
votes
7
replies
1.9k
views
News:
EuroBioC2023 abstract submission is open!
presentation
EuroBioC2023
Conference
community
Bioconductor
written 2.6 years ago by
Maria Doyle
▴ 100
1
vote
4
replies
266
views
DESeq2 interactions
DESeq2
written 25 days ago by
Michael
• 0
0
votes
0
replies
67
views
gene Set with high AUC score but no cells assigned to cell type
AUCell
16 days ago
gogeni5529
• 0
0
votes
0
replies
74
views
GOseq Probability Weight Function bad fit
goseq
16 days ago
francesca
• 0
0
votes
3
replies
227
views
Sharing a collection of datasets in ExperimentHub
Bioconductor
updated 8 days ago by
Aaron Lun
★ 29k • written 17 days ago by
Stephen Piccolo
▴ 600
0
votes
0
replies
117
views
DESeq2 for candidate gene analysis
Bioconductor
19 days ago
piffelpaff
• 0
0
votes
1
reply
86
views
Problem with viewGO(ref_path) in SpliceWiz
Bioconductor
SpliceWiz
20 days ago • updated 16 days ago
jalte.jelsing
• 0
0
votes
0
replies
46
views
Problem with the TMB plot
Bioconductor
20 days ago
nayanajose
• 0
0
votes
3
replies
1.4k
views
problem for finding soft power for constructing network in WGCNA algorithm
RNA-seq
WGCNA package
soft power
written 7.2 years ago by
modarzi
▴ 10
0
votes
0
replies
83
views
After running samtools, the featureCounts command crashed.
RNASeqData
RNASeq
rnaseqGene
22 days ago
Rui
• 0
0
votes
6
replies
327
views
GO.db and others package Download failure
GO.db
Bioconductor
updated 20 days ago by
James W. MacDonald
68k • written 22 days ago by
Skyler
• 0
0
votes
0
replies
7
views
Spam:
Best Rehab Center In India | Luxury, Alcohol & Mental Health Care
COSMIC.67
22 days ago
Best Rehab Center
0
0
votes
2
replies
141
views
replace cat with message in glmTreat when lfc = 0
edgeR
glmTreat
updated 20 days ago by
Gordon Smyth
53k • written 24 days ago by
oliverd
• 0
0
votes
0
replies
56
views
Job:
Research and Service Group Leader positions in AI in Biology at EMBL
Bioconductor
22 days ago
Wolfgang Huber
★ 13k
0
votes
1
reply
87
views
Differential motif analysis
motifmatchr
updated 23 days ago by
James W. MacDonald
68k • written 23 days ago by
Marek Gierlinski
▴ 30
0
votes
0
replies
60
views
News:
Ensembl 115 has been released!
ensembl
ensembldb
biomaRt
24 days ago
Ensembl_Jorge
• 0
0
votes
0
replies
60
views
A problem occurs when running the monocle2
monocle
24 days ago
yuheng_yang
• 0
1
vote
3
replies
283
views
readDNAStringSet with non DNA characters
Biostrings
24 days ago • updated 22 days ago
biomiha
▴ 20
0
votes
2
replies
163
views
readBam vs read_bam_insert_metrics
cfDNAPro
cfDNA
updated 24 days ago by
James W. MacDonald
68k • written 27 days ago by
rtrivedi1
▴ 10
1
vote
3
replies
278
views
How to construct a BSgenome object from a custom genome assembly using BSgenomeForge?
BSgenome
BSgenomeForge
updated 24 days ago by
James W. MacDonald
68k • written 27 days ago by
mat149
▴ 80
3
votes
14
replies
886
views
DESeq2 used with zinbwave weights - Differences on contrasts calculations
zinbwave
DESeq2
written 10 weeks ago by
Aurélie
• 0
5
votes
13
replies
986
views
Guidance on limma for pseudobulk scRNA-seq with multi-factor design
limma
RNASeq
scRNAseq
updated 15 days ago by
Gordon Smyth
53k • written 28 days ago by
abaed
• 0
0
votes
0
replies
80
views
Questions about pseudo counts from RUVSeq
RUVSeq
28 days ago
Yuxiang
• 0
0
votes
1
reply
3.0k
views
Job:
Postdoctoral Research Position in Cancer Genomics at Dana-Farber Cancer Institute/Harvard University
cancer
computational biology
rnaseq
chipseq
Job
written 10.9 years ago by
gcyuan
• 0
0
votes
2
replies
161
views
3-factor DESeq2 trime-series analysis
time-series
DESeq2
29 days ago • updated 28 days ago
gogeni5529
• 0
0
votes
3
replies
278
views
Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
RUVSeq
DESeq2
updated 28 days ago by
Michael Love
43k • written 5 weeks ago by
yongjie.wang
• 0
0
votes
1
reply
424
views
Which of apeglm and ashr may be more appropriate for pseudobulked DESeq2 analysis of single-cell RNA-seq data?
apeglm
DESeq2
updated 4 weeks ago by
Michael Love
43k • written 6 weeks ago by
KS
• 0
0
votes
3
replies
168
views
A0A287BB68 not recognized as UNPROT key in org.Ss.eg.db using AnnotationDbi::select()
org.Ss.eg.db
AnnotationDbi
written 5 weeks ago by
Adam Munhall
• 0
0
votes
4
replies
292
views
voomLmFit fitted log-CPM values
limma
edgeR
updated 4 weeks ago by
Gordon Smyth
53k • written 4 weeks ago by
Lina
▴ 10
0
votes
0
replies
105
views
Extract snp information from genomic regions with biomaRt -> "oaries_snp"
biomaRt
updated 5 weeks ago by
James W. MacDonald
68k • written 5 weeks ago by
Jesus
• 0
1
vote
3
replies
269
views
ROAST compare results with complex design
limma
mroast
roast
CAMERA
written 5 weeks ago by
Jiahao Tian
• 0
0
votes
0
replies
5
views
Spam:
Exploring Robotic Hair Restoration Procedure
COSMIC.67
5 weeks ago
paseje6172
0
0
votes
1
reply
141
views
Length of 'dimnames' [1] not equal to array extent
SingleCellExperiment
singlecelle
scRNAseq
5 weeks ago
Francesco Silvestro
• 0
0
votes
0
replies
98
views
News:
Course: Single-cell analysis with R/Bioconductor
SingleCellExperiment
SingleCell
SingleCellWorkflow
SingleCellData
6 weeks ago
Ludwig Geistlinger
▴ 70
3
votes
5
replies
1.0k
views
How to account for solvent controls in DESeq2 when comparing follicular vs luteal phase?
DESeq2
updated 5 weeks ago by
James W. MacDonald
68k • written 6 weeks ago by
snijesh
▴ 200
0
votes
1
reply
314
views
News:
Bioconductor Board Nominations Open
TAB
Bioconductor
CAB
written 12 weeks ago by
shepherl
4.2k
2
votes
8
replies
547
views
Are voomByGroup and voomLmFit compatible?
limma
voomLmFit
voomByGroup
updated 5 weeks ago by
Gordon Smyth
53k • written 7 weeks ago by
richardallenfriedmanbrooklyn
▴ 30
46,647 results • Page
1 of 933
Recent ...
Replies
Comment: Help with defining groups
by
Phinney, Brett
▴ 10
I got it working , thank you!!!
Comment: Check removeBatchEffect effectiveness
by
massimo
• 0
I would kindly ask for additional support. When not including the design_timepoint argument in removeBatchEffect and thus keeping the inter…
Comment: Error Loading Experiment from SimBenchData Package
by
ksankaran
• 0
Aha, that was it! The object was class Seurat, but I didn't have it installed. After installing, everything works as expected.
Comment: Streamlining the computing time for MiloDE p-value correction in large dataset?
by
ATpoint
★ 5.0k
Is a method that is so computationally expensive really beneficial and necessary? You seem to have biological replication, don't you, so ps…
Answer: NA values for DESeq2 p-values and adjusted p-values using large sample sizes
by
ATpoint
★ 5.0k
Outliers without replacement, independent filtering or all-zero counts for that gene. That's the three options that trigger NAs https://bio…
Votes
Comment: Check removeBatchEffect effectiveness
Gene expression associated with continuous (quantitative) traits (Limma/edgeR/correlation)
Answer: Error Loading Experiment from SimBenchData Package
Comment: incomplete imputation of missing values with LEA impute() function
Answer: Expected memory usage for analyzing large single-cell seq datasets (> 450,000 ce
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