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5
votes
6
replies
418
views
Not fully paired differential gene expression analysis
edgeR
DifferentialExpression
updated 20 days ago by
ATpoint
★ 4.1k • written 24 days ago by
nhaus
▴ 30
0
votes
7
replies
410
views
DESeq filtering specific to contrasts
DESeq2
updated 14 days ago by
Michael Love
41k • written 11 weeks ago by
Emma
• 0
4
votes
6
replies
410
views
FilterByExpr low counts with small sample size
edgeR
16 days ago • updated 11 days ago
Jonathan
▴ 10
2
votes
6
replies
397
views
How to make boxplot with pairwise and overall p value from Limma
t.test
boxplot
limma
anova
25 days ago
Chris
• 0
4
votes
8
replies
387
views
Memory errors using read.bismark?
dmrseq
bsseq
updated 14 days ago by
Peter Hickey
▴ 740 • written 22 days ago by
Juan Higareda
• 0
1
vote
13
replies
382
views
Trying to use enrichGO
clusterProfiler
GenomicDistributionsData
KEGG
12 days ago • updated 9 days ago
fernanda.backsouza
▴ 10
2
votes
2
replies
382
views
[Diffbind] Blacklist error: Error: Invalid Normalization and Unable to apply Blacklist/Greylist
DiffBind
Normalization
22 days ago • updated 21 days ago
Henry
▴ 10
2
votes
9
replies
354
views
Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
DESeq2
RUVSeq
RUVseq
BatchEffect
DES
updated 8 days ago by
James W. MacDonald
65k • written 8 days ago by
Alexandre
• 0
1
vote
3
replies
344
views
Why is my gene not significantly differentially expressed?
limmaGUI
edgeR
limma
updated 18 days ago by
Gordon Smyth
50k • written 23 days ago by
Simran
• 0
0
votes
1
reply
337
views
Gene names from Matrix
BioCor
StarBioTrek
MatrixGenerics
updated 23 days ago by
Lluís Revilla Sancho
▴ 730 • written 7 months ago by
karmasstark
• 0
0
votes
8
replies
333
views
DESeq2 output used for PCA plot on R studio
DESeq2
RSTUDIO
PCAtools
updated 8 days ago by
swbarnes2
★ 1.3k • written 10 days ago by
Aaliya
• 0
0
votes
6
replies
332
views
unable to access Annotationhub latest record
DNAMethylation
AnnotationHub
EPICv2manifest
annotation
updated 22 days ago by
Tim Peters
▴ 180 • written 4 weeks ago by
s.malik
• 0
3
votes
3
replies
326
views
permutation test in edgeR
rna-seq
edgeR
updated 12 days ago by
Gordon Smyth
50k • written 25 days ago by
Netanel
• 0
2
votes
3
replies
321
views
ComplexHeatmap: add pch to rowAnnotation when the input is a data frame with variable number of continuous columns
ComplexHeatmap
18 days ago • updated 17 days ago
daniel.carbajo
• 0
1
vote
4
replies
308
views
Opposite sign of LFC in count plots of DEGs (DESeq2)
DESeq2
9 days ago • updated 7 days ago
winwater0928
• 0
0
votes
4
replies
304
views
Help with design.matrix
limma
28 days ago • updated 27 days ago
andrea.rodriguez-martinez13
• 0
1
vote
7
replies
302
views
Math expression in shapeCustom legend in EnhancedVolcano
EnhancedVolcano
8 days ago • updated 7 days ago
pl23
• 0
3
votes
1
reply
302
views
Use of negative binomial model for exponential decay rate estimation using DESeq (or similar)
DESeq2
updated 9 days ago by
Michael Love
41k • written 10 days ago by
i.sudbery
▴ 40
0
votes
7
replies
302
views
Methylation Analysis experiment design using Limma
DNAMethylation
limma
methylationArrayAnalysis
16 days ago • updated 10 days ago
hortense96
• 0
0
votes
2
replies
294
views
Error in if (nrow(peaks) > 0) { : argument is of length zero Calls: CopywriteR ... tryCatch -> tryCatchList -> tryCatchOne -> <Anonymous> Execution h…
CopywriteR
CopyhelpeR
4 weeks ago • updated 28 days ago
fer_talavera
• 0
0
votes
5
replies
287
views
Error for AnnotationForge makeOrgPackageFromNCBI function
OrgDb
AnnotationForge
updated 9 days ago by
James W. MacDonald
65k • written 12 days ago by
Gayatri
• 0
1
vote
0
replies
273
views
Tutorial:
Installing/switching between versions of R/Rstudio/Bioconductor on personal machine (Linux | Ubuntu)
bioconductor
Install
14 days ago
BioinfGuru
▴ 30
3
votes
6
replies
267
views
Surrogate variable analysis for paired RNA seq experiment
RUVSeq
sva
22 days ago
nhaus
▴ 30
0
votes
4
replies
261
views
DEG with low counts
DESeq2
updated 14 days ago by
Michael Love
41k • written 19 days ago by
Rajat
• 0
0
votes
2
replies
254
views
Odd behavior of Rsubread::featureCounts when setting `nonOverlap = 0`
featureCounts
Rsubread
4 weeks ago • updated 14 days ago
isaac.vock
• 0
1
vote
2
replies
253
views
CombineArrays for EPIC and EPIC V2
EPICV1
Methylation
combineArrays
minifi
EPICv2manifest
4 weeks ago • updated 1 hour ago
Kim
• 0
0
votes
3
replies
250
views
A ComBat-seq issue: the covariates are confounded
ComBat-seq
sva
updated 15 days ago by
W. Evan Johnson
▴ 850 • written 21 days ago by
schlumbohm
• 0
2
votes
5
replies
246
views
get BM error
ensembldb
updated 9 days ago by
James W. MacDonald
65k • written 9 days ago by
Fara
• 0
1
vote
3
replies
244
views
Lib sizes in the DGE object are different from original lib sizes
edgeR
DifferentialExpression
updated 27 days ago by
James W. MacDonald
65k • written 28 days ago by
Shaimaa Gamal
• 0
1
vote
2
replies
239
views
How to retrieve detailed regression results from deseq2
DESeq2
22 days ago • updated 14 days ago
Weiqian
▴ 10
0
votes
4
replies
239
views
Minfi - estimatecellscount
min
minfi
updated 4 days ago by
James W. MacDonald
65k • written 5 weeks ago by
clacarion
• 0
0
votes
2
replies
238
views
featureCounts reports an error:"featureCounts: input-files.c:2890: SAM_pairer_get_next_read_BIN: Assertion `l_name < 256' failed. Aborted (core dumpe…
metaMSdata
10 days ago • updated 8 days ago
shuwangxinze1996
• 0
0
votes
2
replies
238
views
Regressing out batch effects
DESeq2
updated 23 days ago by
Michael Love
41k • written 29 days ago by
kys91240
• 0
0
votes
4
replies
233
views
Differential peaks that were not identified in diffbind were identified in diseq2
DiffBind
DESeq2
ATACSeq
16 days ago • updated 13 days ago
lonn
• 0
0
votes
3
replies
226
views
log2FoldChange value is way too different when compared with counts(dds)
DESeq2
updated 2 days ago by
Michael Love
41k • written 3 days ago by
HAK
• 0
1
vote
2
replies
225
views
MethylKit read issue
methylKit
updated 28 days ago by
James W. MacDonald
65k • written 4 weeks ago by
CHEN
• 0
2
votes
5
replies
223
views
How to retrieve gene ontology GO class from gene list?
GO.db
goTools
updated 8 days ago by
James W. MacDonald
65k • written 10 days ago by
bandconductor
• 0
1
vote
4
replies
222
views
Error EPICv2 ChAMP
ChAMPdata
IlluminaHumanMethylationEPICv2anno.20a1.hg38
ChAMP
updated 11 days ago by
James W. MacDonald
65k • written 15 days ago by
Mirella Aurora
• 0
0
votes
4
replies
220
views
Storing directories inside an ExperimentHub package?
ExperimentHub
23 days ago • updated 22 days ago
Tim Barry
• 0
3
votes
3
replies
218
views
DESeq2 - dispersion estimation when there is none
DESeq2
updated 10 days ago by
Michael Love
41k • written 11 days ago by
WardDeb
• 0
0
votes
2
replies
215
views
Interaction term vs grouping variable design considerations
DESeq2
DifferentialExpression
updated 23 days ago by
Michael Love
41k • written 28 days ago by
nhaus
▴ 30
0
votes
3
replies
215
views
Assist me in determining whether the analysis process using the limma package has been executed correctly.
GEOquery
ArrayExpress
limma
DifferentialExpression
updated 3 days ago by
James W. MacDonald
65k • written 4 days ago by
SSSJec
• 0
0
votes
1
reply
211
views
How do I select a GC file (gc.wig)? (How to determine the inputdata of HMMcopy)
CopyNumberPlots
HMMcopy
CopyNumberVariation
updated 3 days ago by
James W. MacDonald
65k • written 3 days ago by
SSSJec
• 0
1
vote
2
replies
209
views
significant difference of p-values with dream() after updating VariancePartition
variancePartition
15 days ago
Le
• 0
3
votes
2
replies
206
views
Cannabis and KEGGgraph
KEGGREST
GenomicDistributionsData
KEGGgraph
KEGG
updated 11 days ago by
Guido Hooiveld
★ 3.9k • written 14 days ago by
fernanda.backsouza
▴ 10
1
vote
3
replies
205
views
Different Shrunk Log2FC according with reference
DESeq2
updated 9 days ago by
Michael Love
41k • written 9 days ago by
andrebolerbarros
▴ 20
0
votes
3
replies
202
views
Interaction Terms in DESeq2 - Shrinkage
DESeq2
updated 17 days ago by
Michael Love
41k • written 18 days ago by
andrebolerbarros
▴ 20
0
votes
2
replies
202
views
RNAseq:Technical replicates from different batches
technicalReplicates
BatchEffect
limma
RNASeq
14 days ago • updated 11 days ago
catv
• 0
0
votes
2
replies
201
views
Get genomic coordinates of CpGs sites (chromosomes, genomic position)
GenomeWideAssociation
SequenceAnnotation
MethylationArray
GenomicDistributionsData
2 days ago • updated 1 day ago
Yveto
• 0
3
votes
3
replies
201
views
DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
DESeq2
contrasts
updated 4 days ago by
ATpoint
★ 4.1k • written 4 days ago by
bioinf
▴ 10
205 results • Page
2 of 5
Recent ...
Replies
Comment: CombineArrays for EPIC and EPIC V2
by
Kim
• 0
Thank you Tim, this is a great help in getting me started!
Comment: Too many significant genes when integrating gtex and tcga
by
Michael Love
41k
Yes, this was one of the aspects we highlighted in the 2014 paper, and it's also in the workflow. Check these places first. Also take a st…
Answer: Too many significant genes when integrating gtex and tcga
by
James W. MacDonald
65k
See `?results`, in particular the lfcThreshold argument.
Comment: Once again a "Model matrix not full rank"
by
James W. MacDonald
65k
You already ran a temporal assay. Now you can compare each time point to the baseline. The interaction term is only applicable if you have…
Comment: Timecourse RNASeq analysis
by
Aurora
• 0
I managed to run ImpulseDE2 as I am interested in getting significant gene changes over time. Now I would like to run deseq as well and com…
Votes
Answer: CombineArrays for EPIC and EPIC V2
Answer: Too many significant genes when integrating gtex and tcga
Comment: Too many significant genes when integrating gtex and tcga
A: Print Differentially Expressed Exons From Dexseq Results
stuck on unimplemented type 'list' in 'EncodeElement'
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