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15
votes
23
replies
2.1k
views
The difference when using Limma compare between 3 conditions vs 2 conditions
limma
7 weeks ago • updated 20 days ago
Chris
▴ 10
15
votes
17
replies
1.3k
views
Why p value inconsistent when using different number of pathway score?
limma
4 weeks ago • updated 18 days ago
Chris
▴ 10
10
votes
10
replies
1.9k
views
SGSeq: moving toward diffex from SGSeq analysis
sgseq
rnaseq
updated 4 days ago by
Leonard Goldstein
▴ 220 • written 6.7 years ago by
Sylvain Foisy
▴ 80
7
votes
39
replies
5.8k
views
Ensembl plants at annotationHub?
ensembl
ensembldb
plant
arabidopsis thaliana
annotationhub
updated 2 days ago by
Daniele
• 0 • written 5.5 years ago by
Guido Hooiveld
★ 4.0k
7
votes
6
replies
615
views
Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it does not happen in Linux
GenomAutomorphism
written 14 days ago by
robersy
• 0
6
votes
12
replies
5.1k
views
BiocFileCache/ExperimentHub/AnnotationHub temporary ERROR
BiocFileCache
AnnotationHub
ExperimentHub
6 months ago • updated 28 days ago
shepherl
3.9k
6
votes
19
replies
2.6k
views
DESeq2 a lot of genes showing up as differentially expressed that only have 1 sample with any expression
deseq2
updated 20 days ago by
Sey R.
• 0 • written 4.6 years ago by
hsbio
▴ 10
5
votes
6
replies
2.8k
views
Although I successfully installed the package "simpleaffy'' , I cannot library it. Why?
simpleaffy
updated 18 days ago by
James W. MacDonald
65k • written 2.4 years ago by
yanchangjian2019
• 0
5
votes
3
replies
318
views
Log-cpm values from limma
limma
edgeR
RNASeq
updated 10 days ago by
Gordon Smyth
50k • written 4 weeks ago by
Shaimaa Gamal
• 0
5
votes
8
replies
8.9k
views
Limma design with continuous and categorical covariates
limma
batch effect
covariates
microarray
updated 27 days ago by
hortense96
• 0 • written 7.8 years ago by
dmcleanuk
▴ 30
4
votes
5
replies
337
views
Method to find pathways different between 2 groups
fgsea
GSVA
CAMERA
14 days ago • updated 5 days ago
Chris
▴ 10
4
votes
0
replies
145
views
News:
Bioconductor 3.19 is Released!
Release
Bioconductor
Packages
Software
18 days ago
shepherl
3.9k
4
votes
9
replies
269
views
Can DESeq2 rlog normalized matrix reflects time course result directly?
DESeq2
timecoursedata
GeneExpression
updated 4 days ago by
ATpoint
★ 4.1k • written 5 days ago by
pangtaihin
• 0
4
votes
2
replies
634
views
Why does GSEA on edgeR results for randomized samples give highly significant p-values?
fgsea
edgeR
17 days ago • updated 13 days ago
gene_bioconductor
▴ 10
4
votes
9
replies
3.1k
views
Create protein sequences including variants from a VCF file
VariantAnnotation
customProDB
updated 4 days ago by
Yun
• 0 • written 5.2 years ago by
daniel.magnus.bader
▴ 50
4
votes
6
replies
475
views
FilterByExpr low counts with small sample size
edgeR
4 weeks ago • updated 28 days ago
Jonathan
▴ 10
4
votes
9
replies
494
views
Memory errors using read.bismark?
dmrseq
bsseq
5 weeks ago • updated 13 days ago
Juan Higareda
• 0
3
votes
4
replies
293
views
Store output of matchPWM()
biostrings
Biostrings
19 days ago • updated 6 days ago
mat149
▴ 70
3
votes
3
replies
368
views
permutation test in edgeR
rna-seq
edgeR
updated 29 days ago by
Gordon Smyth
50k • written 6 weeks ago by
Netanel
• 0
3
votes
3
replies
253
views
DESeq2 - dispersion estimation when there is none
DESeq2
updated 27 days ago by
Michael Love
42k • written 28 days ago by
WardDeb
• 0
3
votes
1
reply
329
views
Use of negative binomial model for exponential decay rate estimation using DESeq (or similar)
DESeq2
updated 26 days ago by
Michael Love
42k • written 26 days ago by
i.sudbery
▴ 40
3
votes
1
reply
152
views
How to save the DEXSeq results
DEXSeq
updated 14 days ago by
Michael Love
42k • written 14 days ago by
Sara
• 0
3
votes
2
replies
238
views
Cannabis and KEGGgraph
KEGGREST
GenomicDistributionsData
KEGGgraph
KEGG
updated 28 days ago by
Guido Hooiveld
★ 4.0k • written 4 weeks ago by
fernanda.backsouza
▴ 10
3
votes
3
replies
273
views
DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
DESeq2
contrasts
updated 21 days ago by
ATpoint
★ 4.1k • written 21 days ago by
bioinf
▴ 10
3
votes
1
reply
1.5k
views
Limma without eBayes, is it indistunguishable from Ordinary Least Square?
Limma
ebayes
bayesian
ls
updated 12 days ago by
Gordon Smyth
50k • written 5.6 years ago by
CantExitVIM
▴ 10
3
votes
5
replies
305
views
Help with running egsea()
EGSEA
13 days ago • updated 12 days ago
Chris
▴ 10
3
votes
10
replies
485
views
Extremely small p-values using Limma for proteomic data
Proteomics
limma
updated 10 days ago by
James W. MacDonald
65k • written 13 days ago by
Abbey
• 0
3
votes
4
replies
266
views
Help with error using camera() in limma
CAMERA
limma
20 days ago • updated 19 days ago
Chris
▴ 10
2
votes
5
replies
259
views
How to retrieve gene ontology GO class from gene list?
GO.db
goTools
updated 25 days ago by
James W. MacDonald
65k • written 26 days ago by
bandconductor
• 0
2
votes
2
replies
398
views
Importing a GraphNEL into Cytoscape with RCy3 with error: "Error in cyrestPUT(paste("networks", net.suid, "tables", tbl, sep = "/"), : object 're…
RCy3
8 days ago • updated 3 days ago
Matthew Thornton
▴ 360
2
votes
1
reply
148
views
Trim/Filter out-of-bounds GRanges
GenomicRanges
updated 14 days ago by
Michael Love
42k • written 14 days ago by
mat149
▴ 70
2
votes
3
replies
258
views
Too many significant genes when integrating gtex and tcga
RNA-seq
DESeq2
updated 15 days ago by
ATpoint
★ 4.1k • written 16 days ago by
Reza
• 0
2
votes
7
replies
411
views
deseq2 results
DESeq2
DifferentialExpression
rnaseqGene
DifferentialRegulation
12 days ago • updated 9 days ago
sajadahmad41454
• 0
2
votes
3
replies
279
views
package goseq seems to be not available on the latest version of R
goseq
updated 14 days ago by
Gordon Smyth
50k • written 16 days ago by
Patrycja
▴ 20
2
votes
3
replies
120
views
Problem installing ChAMP on a MacBook
ChAMP
updated 4 days ago by
shepherl
3.9k • written 4 days ago by
Galia
• 0
2
votes
1
reply
184
views
minfi latest update for EPIC V2 chip
minfi
minfiDataEPIC
updated 13 days ago by
James W. MacDonald
65k • written 13 days ago by
e1271094
• 0
2
votes
4
replies
1.7k
views
EGSEA usage with microarray data (with vooma)?
EGSEA
gene set testing
vooma
limma voom
gsea
updated 11 days ago by
Chris
▴ 10 • written 7.4 years ago by
Pekka Kohonen
▴ 190
2
votes
13
replies
493
views
Trying to use enrichGO
clusterProfiler
GenomicDistributionsData
KEGG
28 days ago • updated 25 days ago
fernanda.backsouza
▴ 10
2
votes
2
replies
233
views
fgsea significant result
fgsea
15 days ago • updated 14 days ago
Chris
▴ 10
2
votes
1
reply
83
views
How to design DEXSeq formula for multiple variables
DEXSeq
updated 5 days ago by
Alejandro Reyes
★ 1.9k • written 7 days ago by
Sara
• 0
2
votes
5
replies
313
views
get BM error
ensembldb
updated 26 days ago by
James W. MacDonald
65k • written 26 days ago by
Fara
• 0
2
votes
5
replies
224
views
Error while installing BiocManager::install("org.Vdahliae.eg.db")
org.vdahlaie.eg.db
Bioconductor
5 days ago • updated 3 days ago
Patadù
▴ 10
2
votes
3
replies
134
views
How to correctly adjust for gender in edgeR
design
glm
edgeR
updated 4 days ago by
Gordon Smyth
50k • written 5 days ago by
Guillermo
• 0
2
votes
9
replies
428
views
Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
DESeq2
RUVSeq
RUVseq
BatchEffect
DES
updated 24 days ago by
James W. MacDonald
65k • written 25 days ago by
Alexandre
• 0
2
votes
4
replies
289
views
Error EPICv2 ChAMP
ChAMPdata
IlluminaHumanMethylationEPICv2anno.20a1.hg38
ChAMP
updated 28 days ago by
James W. MacDonald
65k • written 4 weeks ago by
Mirella Aurora
• 0
2
votes
2
replies
1.1k
views
limma for gene expression analysis with big and unbalanced sample size
limma
microarray
statistics
updated 19 days ago by
XW
• 0 • written 8.0 years ago by
tati6406
• 0
2
votes
3
replies
1.8k
views
ChipQC 'names' attribute [9] must be the same length as the vector [2]'
chipqc
updated 6 days ago by
bandconductor
• 0 • written 3.6 years ago by
ZheFrench
▴ 60
2
votes
2
replies
286
views
error in limma , contrast.matrix Number of rows of contrast matrix must match number of coefficients in fit
limma
13 days ago • updated 7 days ago
zzrammal
• 0
2
votes
2
replies
238
views
Perform gene set enrichment analysis using fgsea on two conditions that don't have differential express genes
fgsea
20 days ago
Chris
▴ 10
1
vote
3
replies
892
views
How to tell and visualize up/down regulation of pathways from clusterprofiler gsea output
clusterProfiler
RNASeq
updated 26 days ago by
Guangchuang Yu
★ 1.2k • written 14 months ago by
ruiqi.li
▴ 10
170 results • Page
1 of 4
Recent ...
Replies
Answer: How to run allelic imbalance in apeglm
by
Michael Love
42k
You can put 1 / 1 for the ratio when you are missing counts and then set the weight for that matrix cell to 1e-6. Let me know if that's …
Comment: Numerical differences in DESeq2 output depending on different design matrix cons
by
Ken C
• 0
Got you. Thanks again for your comments.
Comment: DESeq2- Mysterious interaction suppresses DEGs
by
shaunpeterson
• 0
Thanks so much for your comment. Yes, this makes sense to me, especially since the effect of the drug is subtle. But it raises a larger con…
Answer: ERROR; return code from pthread_create() is 22
by
James W. MacDonald
65k
Seems OK to me. ``` > dat <- ReadAffy() > dat AffyBatch object size of arrays=744x744 features (20 kb) cdf=HG-U219 (49386 affyids) …
Answer: LRT, more than two conditions and pairwise comparisons
by
James W. MacDonald
65k
Unless you think the different lines or tissues will have very different within-group variances, you should just analyze together and make …
Votes
Answer: DESeq2- Mysterious interaction suppresses DEGs
Answer: Subsetting RNAseq data | voomaByGroup()
biomaRt not working - lazy table error
Answer: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
Comment: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
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