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News:
Bioconductor 3.16 is Released!
Release
Packages
Bioconductor
Software
3.2 years ago
shepherl
4.3k
3
votes
7
replies
3.0k
views
How to visaulize RNAseq data with edgeR, limma, ...?
r
rnaseq
DifferentialExpression
limma
edger
updated 3.2 years ago by
Gordon Smyth
53k • written 3.2 years ago by
jirkamanuel.petersen
• 0
0
votes
0
replies
1.0k
views
Job:
Academic position in Non-clinical Biostatistics
JobPosting
StatisticalMethod
3.2 years ago
Laurent Gatto
1.6k
0
votes
0
replies
719
views
ConsensusClusterPlus and Scaled Data
ConsensusClusterPlus
3.2 years ago
William
• 0
1
vote
4
replies
1.3k
views
Paired analysis model
DESeq2
3.3 years ago • updated 3.2 years ago
giulia.lopatriello
• 0
0
votes
2
replies
1.7k
views
Is it possible for the legend labels to be different from the Colcustom names?
EnhancedVolcano
enha
3.2 years ago
elefth.pavlos
▴ 40
0
votes
0
replies
1.1k
views
How to perform a cluster analysis in RT-qPCR or HT-qPCR results?
cluster
qpcr
qpcrNorm
clusterProfiler
HTqPCR
3.2 years ago
Javier
• 0
1
vote
1
reply
1.5k
views
searching study metadata in recount3
recount3
updated 3.2 years ago by
Leonardo Collado Torres
★ 1.1k • written 3.3 years ago by
andrew.su
▴ 10
1
vote
0
replies
1.0k
views
Over Representation Analysis using PFAM/InterPro database
PFAM.db
clusterProfiler
3.2 years ago
alexandr.gopanenko
▴ 50
0
votes
0
replies
887
views
TCGAbiolinks GDCprepare produces error in vectbl_as_col_location()
TCGAbiolinks
3.2 years ago
lescano
▴ 10
1
vote
1
reply
1.3k
views
Limit the number of nodes in DESeq2
DESeq2
BiocParallel
updated 3.2 years ago by
ATpoint
★ 5.0k • written 3.2 years ago by
Sebastian
▴ 10
2
votes
3
replies
1.8k
views
system.file() failed to load .gmt file.
pathwayPCA
Gsea
updated 3.2 years ago by
gabriel.odom
• 0 • written 3.2 years ago by
Mikołaj Mierzejewski
• 0
0
votes
0
replies
959
views
Database for exploring annotations of the coding genome at variants, such as candidate regulatory SNPs at disease-associated loci.
SNP
ExomeSeqData
snapcount
SNAData
SNPRelate
3.2 years ago
m610110001
• 0
0
votes
0
replies
1.0k
views
News:
Bioconductor Carpentries instructor training
teaching
Carpentries
3.2 years ago
Maria Doyle
▴ 110
1
vote
0
replies
1.1k
views
Shotgun metagenomics data analysis
Metagenomics
abundance
3.2 years ago
YASIR
▴ 10
0
votes
0
replies
653
views
How does distance in bins calculated? Can it be replaced with distance in bp?
metagene
metagene2
3.2 years ago
sfpacman
• 0
1
vote
1
reply
862
views
DESeq2 results not correct
Mus_musculus_Data
updated 3.2 years ago by
James W. MacDonald
68k • written 3.2 years ago by
Ashish
• 0
0
votes
2
replies
983
views
How to get accession by the name of species
genbank
R
accession
3.2 years ago
wertykus1
• 0
0
votes
6
replies
5.3k
views
limma vs lme4: definition of "random effects"
limma
lme4
mixed
3.2 years ago
leandro
• 0
0
votes
0
replies
978
views
News:
Last day to make commits before Release 3.16
Release
Devel
Bioconductor
release
devel
3.2 years ago
shepherl
4.3k
0
votes
2
replies
2.4k
views
HOMER error: Where is the file?
HOMER
chip-seq
updated 3.2 years ago by
ATpoint
★ 5.0k • written 3.2 years ago by
兆豪
• 0
2
votes
1
reply
1.1k
views
Different sequencing depth between replicates and samples
DESeq2
edgeR
DiffBind
updated 3.2 years ago by
Gordon Smyth
53k • written 3.2 years ago by
cesar.arenasmena
▴ 10
0
votes
3
replies
988
views
limma manual typo
limma
updated 3.2 years ago by
Gordon Smyth
53k • written 3.2 years ago by
jeltje.van.baren
• 0
1
vote
3
replies
1.6k
views
Proteomics-time series with repeated measures
Proteomics
limma
repeatedmeasures
TimeSeriesExperiment
3.3 years ago • updated 3.2 years ago
proteome_AO
• 0
0
votes
2
replies
2.6k
views
ChAMP package:Error in cmdscale(d) : 'k' must be in {1, 2, .. n - 1}
MethylationArrayData
3.2 years ago
shuangshuang
• 0
0
votes
0
replies
774
views
Problem loading ChemmineOB
ChemmineOB
updated 3.2 years ago by
shepherl
4.3k • written 3.2 years ago by
juliev
• 0
0
votes
1
reply
1.6k
views
Bug: MOFA2 sets an environment variable that breaks knit in RStudio
RStudio
MOFA2
bug
knitr
updated 3.3 years ago by
James W. MacDonald
68k • written 3.3 years ago by
Kevin Coombes
▴ 430
0
votes
8
replies
2.3k
views
translate errors in fastq file reading
translate
biostrings
Biostrings
3.3 years ago • updated 3.2 years ago
Brett
• 0
0
votes
3
replies
2.7k
views
Differential Expression Analysis on RNAseq - RSEM expected_count from UCSC RNA-Seq toil recompute
DESeq2
Toil
RSEM
3.3 years ago • updated 3.2 years ago
Niveditha
▴ 20
1
vote
4
replies
3.4k
views
plotCounts to visualize variations of a specific genes along conditions
DESeq2
plotCounts
vst
updated 3.3 years ago by
Michael Love
43k • written 3.3 years ago by
Laia
▴ 10
4
votes
5
replies
2.9k
views
time course microarray data ArrayExpress database
R
GillespieColin
ArrayExpress
import
TimeSeriesExperiment
updated 2.3 years ago by
jacorvar
▴ 40 • written 3.3 years ago by
eraldagjika
▴ 20
2
votes
13
replies
4.2k
views
Too much data for Diffbind
DiffBind
updated 15 months ago by
Ou, Jianhong
★ 1.3k • written 3.3 years ago by
littlefishes20
▴ 10
0
votes
4
replies
1.4k
views
Error installing Bioc
clusterProfiler
Bioconductor
BiocManager
updated 3.3 years ago by
Guido Hooiveld
★ 4.1k • written 3.3 years ago by
Javier
• 0
0
votes
3
replies
4.7k
views
Error: BiocParallel errors 1 remote errors, element index: 1 506 unevaluated and other errors first remote error:
methylationArrayAnalysis
updated 2.5 years ago by
olatunbosun.arowolo
• 0 • written 3.3 years ago by
shuangshuang
• 0
1
vote
4
replies
1.6k
views
RNA-seq analysis between a wildtype vs a double microRNA kockout mouse brain
microRNA
gene
RNASeqRData
edgeR
geneontology
3.3 years ago
faiza
• 0
1
vote
1
reply
1.3k
views
creating a NOT gate for gating out a single quadrant in a quadrant gate openCyto
FlowCytometry
Opencyto
updated 3.3 years ago by
Kevin Blighe
★ 4.0k • written 3.3 years ago by
Noël
• 0
3
votes
12
replies
3.7k
views
Replicates in DESeq2
DESeq2
updated 3.2 years ago by
Yijing
• 0 • written 4.5 years ago by
leo.dagata
▴ 10
1
vote
2
replies
1.6k
views
Design formula with batch effects DESeq2 "Not full rank matrix" error.
DESeq2
BatchEffect
RNASeq
updated 3.3 years ago by
swbarnes2
★ 1.4k • written 3.3 years ago by
Flavio
• 0
1
vote
2
replies
1.0k
views
A quick question on “Biostrings” package
local
Smith-Watermanmatrix;
Biostrings;
3.3 years ago • updated 3.2 years ago
happymonan
• 0
1
vote
3
replies
1.2k
views
What should I do with RNA-Seq data to get a gene that work?
find
RNASeqRData
updated 3.3 years ago by
ATpoint
★ 5.0k • written 3.3 years ago by
yuhang
• 0
0
votes
4
replies
1.2k
views
DESeq2 design with continuous variables AND filtering of low counts
DESeq2
updated 3.3 years ago by
Michael Love
43k • written 3.3 years ago by
bgurdon
• 0
0
votes
0
replies
882
views
gate_quad_sequential as parent gate for downstream gating in opencyto CSV template
opencyto
FlowCytometry
3.3 years ago
Noël
• 0
3
votes
1
reply
1.2k
views
Should I set a seed prior to running AUCell?
AUCell
updated 3.3 years ago by
ATpoint
★ 5.0k • written 3.3 years ago by
Lucy
▴ 60
0
votes
2
replies
1.3k
views
kinToMatrix creates an empty matrix (except for diagonal 0.5 values)
kingToMatrix
GENESIS
Plants
3.3 years ago
Monica
• 0
1
vote
11
replies
3.3k
views
Use local Txome database to avoid downloading GTF from FTP server
tximeta
updated 3.3 years ago by
Michael Love
43k • written 3.3 years ago by
Hasher
• 0
2
votes
8
replies
2.1k
views
Errors when plotting data using heatmap_GO (GOexpress)
GOexpress
updated 3.3 years ago by
kevin.rue
▴ 350 • written 3.3 years ago by
grolo
• 0
2
votes
4
replies
1.7k
views
DiffBind and peak counts as input
DESeq2
edgeR
DiffBind
updated 3.2 years ago by
Rory Stark
★ 5.2k • written 3.3 years ago by
Bogdan
▴ 670
2
votes
6
replies
12k
views
Unable to download packages from Bioconductor
downloading-issue
updated 3.2 years ago by
W
• 0 • written 3.6 years ago by
Aryan
▴ 10
2
votes
2
replies
1.1k
views
2-Way Repeated Measures ANOVA - Affymetrix
AffymetrixChip
MicroarrayData
Anova
3.3 years ago
evan.j.williams
▴ 10
3
votes
12
replies
3.9k
views
t.test to determine the P-value in DESeq2
DESeq2
written 3.3 years ago by
Maryam.sed1400
• 0
46,732 results • Page
73 of 935
Recent ...
Replies
Answer: Array weights in dpcDE
by
Gordon Smyth
53k
The uncertainty of quantification is fully accounted for in the dpcDE() analysis, including in the computation of sample weights. Your use…
Answer: lfc shrinkage estimator in DESeq2
by
Michael Love
43k
Try the vignette which has a lot of information on these type of questions.
Comment: limpa analysis advice
by
Gordon Smyth
53k
The DPC slope is beta1. The estimated slopes for your data look pretty low, lower than I've ever used for a real dataset. Nevertheless, as …
Comment: limpa analysis advice
by
SamGG
▴ 360
Thanks for your feedback and for reassuring me. :-) As you suggested, I tried dpcCN() and set the slope to 0.7 in addition to the standard…
Comment: Making a Full Rank Model for Allele-Specific Expression with DESeq2
by
chp265
• 0
Good point, I hadn't considered the fact quantitative `time` implies a (logit-)linear response *to* time. You're correct that I haven't exp…
Votes
A: DESeq2: Is it possible to convert read counts to expression values via TPM and r
A: Filtering read counts matrix: how to deal with duplicated gene symbols, differen
Comment: limpa analysis advice
Answer: When to use edgeR or limma
Answer: When to use edgeR or limma
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