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Transcriptomics
•
reset
2
votes
4
replies
2.5k
views
Altered (negative or positive) logFC values for same gene from two intitial count matrices, one being a subset of the other
Transcriptomics
edgeR
DEG
3 months ago
Maziya
• 0
0
votes
3
replies
821
views
DESeq2 analysis with transcript counts
Transcriptomics
RNASeq
DESeq2
written 8 months ago by
sindhuri
• 0
0
votes
0
replies
255
views
Filtering Steps for proActiv Analysis
Transcriptomics
proActiv
10 months ago
LuciaNhu
• 0
1
vote
6
replies
1.6k
views
New to Deseq2 - DEG questions / MA plot
genomes
DESeq2
Transcriptomics
updated 10 months ago by
ATpoint
★ 4.9k • written 11 months ago by
Newbie
• 0
0
votes
0
replies
404
views
proActiv-promoter analysis
proActiv
Transcriptomics
13 months ago
wang.xiangyu
• 0
0
votes
0
replies
741
views
Job:
Postdoctoral fellowships in Spatial Omics and Single Cell Genomics at the University of Padova (IT)
SingleCell
Transcriptomics
SpatialDatasets
SpatialData
15 months ago
Silvio
• 0
3
votes
2
replies
684
views
How to extract counts from a DESeq dataset ordered by a specific sample?
DESeq2
Transcriptomics
17 months ago
Anita
▴ 10
4
votes
5
replies
3.7k
views
DESeq2, time-course interpretation
Transcriptomics
TimeCourse
DESeq2
updated 2.2 years ago by
Michael Love
43k • written 2.2 years ago by
smac_head
• 0
0
votes
4
replies
1.3k
views
problem while retriving the genes by using p value and log2fc as conditions
Bioconductor
Transcriptomics
DifferentialExpression
DESeq2
2.3 years ago
sreeshmaraj72
• 0
0
votes
1
reply
667
views
Time change in expression vs time change in phenotype
Transcriptomics
ExpressionData
DESeq2
updated 2.4 years ago by
Michael Love
43k • written 2.4 years ago by
Ivan
• 0
4
votes
5
replies
3.5k
views
EnhancedVolcano - Unable to customize the length of Connectors to Gene Labels
Transcriptomics
EnhancedVolcano
updated 2.4 years ago by
Kevin Blighe
★ 4.0k • written 2.4 years ago by
Peter
▴ 20
4
votes
8
replies
2.6k
views
DESEQ2 - What is the recommended workflow in terms of pre-filtering and counts normalization?
DESeq2
Transcriptomics
updated 2.4 years ago by
Wolfgang Huber
★ 13k • written 2.4 years ago by
Peter
▴ 20
0
votes
3
replies
1.3k
views
Understanding edgeR differential expression results
Transcriptomics
edgeR
DifferentialExpression
RNASeqData
RNASeq
updated 2.5 years ago by
Gordon Smyth
53k • written 2.5 years ago by
Teresa
• 0
0
votes
1
reply
1.2k
views
Is it possible to convert transcript-level to gene-level dataset to use it in DESeq2 ?
DESeq2
Transcriptomics
tximport
deseq
txi
updated 2.7 years ago by
Dario Strbenac
★ 1.6k • written 2.7 years ago by
Hicham
▴ 10
0
votes
1
reply
2.8k
views
Variance stabilization transformation (VST), blind=TRUE
DESeq2
Transcriptomics
RNASeq
updated 2.7 years ago by
Michael Love
43k • written 2.7 years ago by
Jayesh Kumar
• 0
0
votes
0
replies
939
views
Analysis of TIF-seq data to generate transcription start sites (TSS) and poly(A) site (PAS) clusters
Transcriptomics
SequencingData
2.8 years ago
Rom
• 0
0
votes
0
replies
1.0k
views
Job:
EPA Postdoctoral Fellowship in Bioinformatics and Computational Toxicology
MethylSeq
Transcriptomics
Pathways
Epigenetics
RNASeq
2.8 years ago
whduke
• 0
0
votes
2
replies
1.0k
views
Questions about `collapseReplicates` function in DESeq2
DESeq2
Transcriptomics
2.9 years ago
Yijing
• 0
0
votes
0
replies
1.0k
views
WCGNA: Using Eigengenes when correlating modules to external features? What about PC2, PC3???
Network
Transcriptomics
MicrobiomeData
RNASEQ
WCGNA
3.0 years ago
Julieta
• 0
3
votes
1
reply
3.7k
views
Understanding tximport working with salmon outputs
Transcriptomics
tximport
updated 3.0 years ago by
ATpoint
★ 4.9k • written 3.0 years ago by
Florent
▴ 10
0
votes
3
replies
1.7k
views
DESeq2 - transcript length correction necessary when quantifying reads from ends of transcripts?
Transcriptomics
DESeq2
3.1 years ago
marius.wenzel
• 0
4
votes
6
replies
2.2k
views
How to best visualize multi-level transcriptomics experiment data?
ggplot2
Transcriptomics
Visualization
R
limma
3.3 years ago
mohammedtoufiq91
▴ 10
1
vote
3
replies
1.8k
views
Identifying DEG between development stages
plant
Transcriptomics
DESeq2
r
updated 3.5 years ago by
Basti
▴ 780 • written 3.5 years ago by
cicely.day
• 0
2
votes
5
replies
1.9k
views
DESeq2 with baseline correction for gene expression differences
DESeq2
edgeR
Transcriptomics
updated 3.5 years ago by
Michael Love
43k • written 3.5 years ago by
R1337
• 0
2
votes
2
replies
1.2k
views
Deseq2 Resul variation by LFC variation
rnaseqGene
DESeq2
Transcriptomics
3.9 years ago • updated 2.3 years ago
diangomaiga
• 0
1
vote
2
replies
1.9k
views
Not many DE genes using glmTreat
EdgeR
Bioinformatics
Transcriptomics
Quantseq
4.1 years ago
Barista
• 0
0
votes
0
replies
1.3k
views
Job:
Senior Bioinformatician - University of Alabama at Birmingham
JobPosting
Transcriptomics
SingleCell
RNA-seq
4.1 years ago
lianov
• 0
0
votes
0
replies
1.2k
views
Job:
[apply by 8/15] PhD scholarship on functional and evolutionary genomics
JobPosting
Transcriptomics
GenomicVariation
4.2 years ago
marie
• 0
0
votes
2
replies
1.8k
views
Downloading org.Mm.eg.db after R reinstall
org.Mmu.eg.db
Transcriptomics
4.4 years ago
Kate
• 0
0
votes
0
replies
1.3k
views
Consensus wgcna soft threshold for each data
Transcriptomics
WGCNA
wgcna
4.5 years ago
2323982403
▴ 60
0
votes
1
reply
1.1k
views
Metatranscriptomics DESeq2 multifactorial sampling
Transcriptomics
DESeq2
updated 4.5 years ago by
Michael Love
43k • written 4.5 years ago by
seppe.demits
• 0
0
votes
0
replies
1.3k
views
Job:
Bioinformatician at MiroBio, Oxford, UK
Transcriptomics
Bioconductor
Statistics
Job
Biocon
4.5 years ago
m.jackson
• 0
5
votes
5
replies
4.5k
views
analyzing RNA-Seq transcriptomic data (GEO accession: GSE96058)
GEO
RNASeqR
Transcriptomics
updated 4.5 years ago by
James W. MacDonald
68k • written 4.5 years ago by
H. Z. Amini
▴ 10
0
votes
0
replies
1.4k
views
Job:
Computational Biologist at Denali Therapeutics
Transcriptomics
SingleCell
JobPosting
4.7 years ago
Steve Lianoglou
★ 13k
1
vote
1
reply
1.3k
views
NanoStringDiff Not Recognized as Numeric
Bioconductor
Transcriptomics
NanoStringDiff
updated 4.9 years ago by
Laurent Gatto
1.6k • written 4.9 years ago by
Pedro
▴ 10
0
votes
0
replies
1.0k
views
Problems with cummeRbund
plot
transcriptomics
differential expression
cummeRbund
5.5 years ago
raquelponti
• 0
1
vote
0
replies
1.4k
views
News:
News: Bioinformatics Summer School - July 2020 - UCLouvain (Belgium)
rnaseq
course
proteomics
transcriptomics
scrnaseq
News
5.6 years ago
Laurent Gatto
1.6k
0
votes
4
replies
1.6k
views
GOexpress - Error when plotting expression data
RNA-seq
transcriptomics
gene ontology
gene expression
GOexpress
updated 5.6 years ago by
kevin.rue
▴ 350 • written 5.6 years ago by
atorson
• 0
0
votes
0
replies
1.3k
views
Job:
Bioinformatics Scientist, CNS Transcriptomics at Alnylam Pharmaceuticals, Cambridge MA, USA
scRNA-seq
CNS
transcriptomics
RNAi
Job
5.7 years ago
Joseph Barry
▴ 10
0
votes
0
replies
1.0k
views
Job:
Bionformatician in personalized oncotherapy (BEL)
job
bioinformatician
immunotherapy
genomics
transcriptomics
Job
5.8 years ago
cedric
• 0
1
vote
0
replies
1.2k
views
Job:
Bioinformatics PhD: The circadian clock in the ageing plant, University of York, UK
transcriptomics
gene expression
time series
plant biology
circadian
Job
5.9 years ago
dezer
▴ 10
2
votes
2
replies
4.4k
views
How adjusted p-values are calculated in limma
limma
r
transcriptomics
FDR
statistics
updated 6.0 years ago by
Gordon Smyth
53k • written 6.0 years ago by
Wuschel
▴ 10
2
votes
0
replies
2.7k
views
Job:
Bioconductor infrastructure for the integration of genomics, transcriptomics and proteomics data
proteomics
genomics
transcriptomics
software
Job
6.0 years ago
Laurent Gatto
1.6k
1
vote
1
reply
1.4k
views
cpm filtering parameter
cpm filtering
transcriptomics
rnaseq
edgeR
metatranscriptomics
updated 6.0 years ago by
Gordon Smyth
53k • written 6.0 years ago by
vm.higareda
▴ 10
0
votes
0
replies
1.3k
views
Job:
Bionformatician Position / Heidelberg
neuroscience
data integration
transcriptomics
Job
6.5 years ago • updated 6.4 years ago
slezak
• 0
0
votes
0
replies
1.1k
views
How to integrate and visualize RNASeq / Proteomics data for metabolomics pathways?
r
metabolomics
KEGG
Proteomics
Transcriptomics
6.5 years ago
Wuschel
▴ 10
1
vote
0
replies
1.9k
views
Job:
Full time and permanent research logistician in Bioinformatics
transcriptomics
proteomics
job
bioinformatician
Job
6.5 years ago
Laurent Gatto
1.6k
1
vote
1
reply
1.8k
views
News:
Bioinformatics Summer School - July 2019 - UCLouvain (Belgium)
RNAseq
deseq2
proteomics
transcriptomics
workshop
News
written 6.5 years ago by
Laurent Gatto
1.6k
3
votes
4
replies
1.8k
views
Writing a loop to clean up protein names
Proteomics
Transcriptomics
Loops
batch-processing
R
updated 6.9 years ago by
Sebastian Gibb
▴ 80 • written 6.9 years ago by
laural710
• 0
49 results • Page
1 of 1
Recent ...
Replies
Comment: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
Michael Love
43k
I moved your post from an "answer" to a "comment". I don't know about `offst()` function, what is the scale or direction, or whether you a…
Comment: Average biological replicates in RNA-seq
by
boczniak767
▴ 740
@atpoint Should I first standardise data and then average replicates?
Comment: 3-factor DESeq2 trime-series analysis
by
gogeni5529
• 0
Thans you @swbarnes2 for the answer. I can see why trying to include all three parameters might be difficult to interpret. But we're inte…
Comment: 3-factor DESeq2 trime-series analysis
by
swbarnes2
★ 1.4k
What does your PCA look like? A lot of times, I find that different cell lines are so different from each other, that they should not be d…
Comment: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
yongjie.wang
• 0
Thanks for your reply. " normFactors <- exp(-1 * offst(set2)) normFactors <- normFactors / exp(rowMeans(log(normFactors))) normalizat…
Votes
Answer: Limpa Pipeline Order
A: How barcode-plot enrichment is calculated?
A: How barcode-plot enrichment is calculated?
Answer: ROAST compare results with complex design
How to account for solvent controls in DESeq2 when comparing follicular vs luteal phase?
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