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geneexpression
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120
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WGCNA sample-data preprocessing order
Microarray
WGCNA
GeneExpression
updated 4 days ago by
ATpoint
★ 4.6k • written 9 days ago by
Reuben
• 0
2
votes
1
reply
226
views
Using Affymetrix Custom Array
ArrayExpress
makecdfenv
AffymetrixChip
gcrma
GeneExpression
updated 10 weeks ago by
James W. MacDonald
67k • written 10 weeks ago by
Piotr.Keller
• 0
0
votes
1
reply
271
views
Is my time course analysis with DESeq2 valid?
GeneExpression
DESeq2
TimeCourse
updated 10 weeks ago by
Michael Love
43k • written 10 weeks ago by
Jason
• 0
4
votes
9
replies
872
views
Can DESeq2 rlog normalized matrix reflects time course result directly?
DESeq2
timecoursedata
GeneExpression
updated 8 months ago by
ATpoint
★ 4.6k • written 8 months ago by
pangtaihin
• 0
2
votes
3
replies
771
views
"could not find function "readTSGE" in package ctsGE" Error occurs when trying to follow ctsGE package instructions.
GeneExpression
Clustering
ctsGE
11 months ago
jbgnielson
• 0
2
votes
2
replies
741
views
DESeq2: Comparison of single clinical sample to 4 normals using tumour cohort to infer dispersion of single sample
GeneExpression
DESeq2
RNASeqData
RNASeq
StatisticalMethod
14 months ago
Manthos
• 0
2
votes
3
replies
3.6k
views
TPM from STAR mapped counts
GeneExpression
GenomicFeatures
RNA-Seq
TPM
updated 16 months ago by
Gordon Smyth
52k • written 16 months ago by
gingergeiger22
• 0
0
votes
1
reply
796
views
Read methylation data from tar files on GEO (GSE73303)
GeneExpression
Bioconductor
GEOquery
MethylationArrayData
18 months ago
Arianna
• 0
2
votes
2
replies
2.9k
views
FeatureCounts Output Counts at Exon Level Using Default Settings, want gene level
GeneExpression
21 months ago
CDSPARKS
▴ 30
0
votes
1
reply
912
views
Heatmap of two conditions from the normalized gene count data
GeneExpression
heatmaps
updated 23 months ago by
ATpoint
★ 4.6k • written 23 months ago by
Amazing570
• 0
0
votes
1
reply
3.8k
views
DESeq2 after Seurat analysis on integrated datasets
DESeq2
SingleCell
GeneExpression
Seurat
updated 23 months ago by
ATpoint
★ 4.6k • written 23 months ago by
bio.boi
• 0
3
votes
4
replies
2.3k
views
Comparing different method for DESEQ2
DESeq2
GeneExpression
updated 2.3 years ago by
Michael Love
43k • written 2.4 years ago by
Vapin
• 0
0
votes
3
replies
1.4k
views
CountOverlap
GeneExpression
ChIPSeqData
updated 2.3 years ago by
James W. MacDonald
67k • written 2.3 years ago by
michela
▴ 20
0
votes
0
replies
601
views
Free Gibbson energy RNA doublex
GeneExpression
RNAinteract
2.5 years ago
Tamina
• 0
1
vote
0
replies
792
views
Error while running Case study n. 3: Integration of methylation and expression for ACC
geneexpression
methylation
2.6 years ago
hlee
▴ 10
0
votes
2
replies
1.2k
views
Seeking help in multifactor differential gene expression analysis using DESeq2. Can not get significance difference while comparing 3 time factor (…
DifferentialExpression
GeneExpression
R
enrichment
DEGseq
updated 2.6 years ago by
swbarnes2
★ 1.4k • written 2.6 years ago by
ac.mainul.mmb
• 0
0
votes
1
reply
771
views
Error "DESeqDataSetFromMatrix" function not found
GeneExpression
updated 2.6 years ago by
Basti
▴ 780 • written 2.6 years ago by
Iraide
• 0
0
votes
1
reply
856
views
Aggregate differentially expressed genes across all contrast results using DEVis
DifferentialExpression
NanoporeRNASeq
DESeq2
GeneExpression
DEVis
updated 2.7 years ago by
James W. MacDonald
67k • written 2.7 years ago by
Ana
• 0
1
vote
1
reply
1.0k
views
Error while using champ.SVD function
GeneExpression
updated 2.8 years ago by
Basti
▴ 780 • written 2.8 years ago by
Afrin
• 0
0
votes
1
reply
2.2k
views
GDCprepare of STAR - counts
GeneExpression
TCGAbiolinks
updated 2.4 years ago by
Aurora
• 0 • written 2.8 years ago by
mahsa
• 0
0
votes
0
replies
1.1k
views
Volcano plot: Putting calculated values in legend
GeneExpression
updated 2.8 years ago by
Kevin Blighe
★ 4.0k • written 2.8 years ago by
Narender Singh
• 0
2
votes
2
replies
1.0k
views
Could use some advice on analyzing ranks of expressed genes in two conditions,
GeneExpression
DESeq2
ExpressionData
updated 2.8 years ago by
fivescarynightsgames
• 0 • written 2.9 years ago by
reza
▴ 10
0
votes
2
replies
2.5k
views
Using DESeq2 for Differential Expression Analysis with Interaction Term
DifferentialExpression
GeneExpression
DESeq2
Design()
3.3 years ago
p.l.chen
• 0
2
votes
2
replies
7.9k
views
Deseq2 and log2foldchange cutoffs- How to get differentially expressed genes?
DifferentialExpression
GeneExpression
DESeq2
R
updated 3.3 years ago by
ATpoint
★ 4.6k • written 3.3 years ago by
janjoy
▴ 10
0
votes
2
replies
1.3k
views
Gene expression in R
limma
edgeR
Biobase
GeneExpression
3.4 years ago
sogol.bouyeh
• 0
2
votes
2
replies
2.0k
views
DESeq2 - one groups with no replicates
DESeq2
rnaseqGene
DifferentialExpression
GeneExpression
3.6 years ago
SUMIT
• 0
5
votes
18
replies
3.5k
views
DESeq2 comparison two cell types and two genotypes
GeneExpressionWorkflow
DESeq2
GeneExpression
updated 3.7 years ago by
Michael Love
43k • written 3.8 years ago by
Yosapol
• 0
1
vote
3
replies
1.2k
views
DESeq analysis with Biological replicate.
DESeq2
ExpressionData
GeneExpression
updated 4.0 years ago by
Michael Love
43k • written 4.0 years ago by
Javad_Sadeghi
• 0
1
vote
1
reply
872
views
Accounting for fish body mass in DE?
GeneExpression
edgeR
updated 4.1 years ago by
James W. MacDonald
67k • written 4.1 years ago by
michael.lawrence
• 0
0
votes
1
reply
892
views
Find significant genes related to a specific disease
GeneExpression
RNASeqR
limma
updated 4.1 years ago by
António Miguel de Jesus Domingues
▴ 510 • written 4.1 years ago by
Will
• 0
1
vote
3
replies
1.2k
views
Troubles implementing LIMMA for paired samples (before/after treatment) comparaisons
GeneExpression
R
limma
MultipleComparison
updated 4.2 years ago by
James W. MacDonald
67k • written 4.2 years ago by
Gwénolé
• 0
0
votes
0
replies
767
views
Diffrential Gene expression in a group of specific methylated prometor patience
GeneExpression
methyAnalysis
MethylationArray
4.2 years ago
coobzie
• 0
2
votes
5
replies
13k
views
Calculating FPKM from raw counts data using fpkm() in deseq2
deseq2
r
fpkm()
geneexpression
updated 6.3 years ago by
Michael Love
43k • written 6.3 years ago by
Beginner
▴ 60
1
vote
4
replies
2.4k
views
Need help loading gene expression data into R using either ArrayExpress or GEOquery R packages
arrayexpress
geoquery
geneexpression
updated 6.8 years ago by
Sean Davis
21k • written 6.8 years ago by
arbet003
▴ 10
3
votes
1
reply
2.1k
views
Processing several GSM from GEO database using Barcode and fRMA
frma
barcode
geneexpression
gsm
ncbi geo
updated 7.2 years ago by
Matthew McCall
▴ 830 • written 7.2 years ago by
diana.elassal
• 0
1
vote
4
replies
2.1k
views
ddCT Package for StepOnePlus qPCR output
qPCR
ddCt
geneexpression
updated 7.5 years ago by
Zhang, Jitao David
▴ 120 • written 7.5 years ago by
rbronste
▴ 60
1
vote
3
replies
1.4k
views
General suggestions regarding gene expression
geneexpression
updated 8.0 years ago by
Gordon Smyth
52k • written 8.0 years ago by
mariagiovannetti86
• 0
0
votes
0
replies
1.1k
views
Where or how to find the gene interaction with itself?
microarray
gene interaction
geneexpression
8.1 years ago
shaheryar
• 0
0
votes
0
replies
1.3k
views
Error in SAMseq analysis with R
r
samseq
microarray
rnaseq
geneexpression
8.4 years ago
namilio
• 0
0
votes
0
replies
1.4k
views
Methylation and gene expression
methylation
geneexpression
8.5 years ago
Asma rabe
▴ 290
0
votes
2
replies
1.3k
views
Methylation and gene expression data
methylation
geneexpression
updated 8.7 years ago by
arfranco
▴ 130 • written 8.7 years ago by
Asma rabe
▴ 290
0
votes
3
replies
1.8k
views
error in drawing MDS plot for eset
MDSplot
geneexpression
insilicomerging
updated 8.9 years ago by
James W. MacDonald
67k • written 8.9 years ago by
Shamim Sarhadi
▴ 20
1
vote
6
replies
2.1k
views
how to deal the effect of enormous gene expression?
deseq2
rnaseq
tomato
geneexpression
updated 8.9 years ago by
Michael Love
43k • written 8.9 years ago by
hamaor
• 0
0
votes
1
reply
1.5k
views
Error in MetaQC R package.
r
metaqc
microarray
geneexpression
9.1 years ago
Agaz Hussain Wani
▴ 260
0
votes
3
replies
2.2k
views
problem with WGCNA
geneexpression
updated 9.2 years ago by
Peter Langfelder
★ 3.0k • written 9.2 years ago by
nazaninhoseinkhan
• 0
2
votes
5
replies
1.9k
views
heatmap in R
r language
microarray
geneexpression
updated 9.4 years ago by
Axel Klenk
★ 1.0k • written 9.4 years ago by
ifaizurahredzuan
• 0
1
vote
1
reply
1.3k
views
gene expression data analysis
microarray
geneexpression
updated 9.4 years ago by
James W. MacDonald
67k • written 9.4 years ago by
ifaizurahredzuan
• 0
1
vote
1
reply
5.5k
views
NA p and p adjusted value
geneexpression
updated 9.5 years ago by
Peter Langfelder
★ 3.0k • written 9.5 years ago by
timarogger
• 0
1
vote
2
replies
6.5k
views
explanation of makeContrasts function of limma package
limma
microarray
R
geneexpression
updated 9.5 years ago by
Aaron Lun
★ 28k • written 9.5 years ago by
elisa.micarelli
▴ 10
0
votes
3
replies
12k
views
How to combine expression values of multiple probes for one gene
probe
convert
geneexpression
onechannelgui
updated 9.5 years ago by
svlachavas
▴ 840 • written 9.5 years ago by
ayanava18
▴ 10
50 results • Page
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Comment: How to visualize these pathways using pathview
by
Guido Hooiveld
★ 4.1k
FYI: a similar, if not identical issue has been reported before (`pathview` has problems visualizing some pathways, but not all). You likel…
Comment: Why select top highly expressed genes based on normalized counts, but then plot
by
Quang NN
▴ 10
I see. Thank you ATpoint!
Comment: how should I apply "cpg.annotate" to TCGA methylation data in hg38 for HM450K?
by
Prakash Sah
• 0
Thanks James!
Comment: Why select top highly expressed genes based on normalized counts, but then plot
by
ATpoint
★ 4.6k
I think I explained what the differences between approach 1 and 2 is in my answer. I assume that the tutorial uses vst because it is varian…
Comment: Why select top highly expressed genes based on normalized counts, but then plot
by
Quang NN
▴ 10
Thank you ATpoint for your responses. I am a bit confused because the difference between the two approaches is the selection of the genes…
Votes
Bioconductor Course, Kenya 2025: Applications Open!
Comment: can't install tscR
Answer: Csaw normalization strategies - general inquiry
A: DESeq2 interaction term
Comment: Recount3 data correspond to TCGA data
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