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Views
Votes
2
votes
13
replies
434
views
Trying to use enrichGO
clusterProfiler
GenomicDistributionsData
KEGG
17 days ago • updated 13 days ago
fernanda.backsouza
▴ 10
5
votes
6
replies
433
views
Not fully paired differential gene expression analysis
edgeR
DifferentialExpression
updated 25 days ago by
ATpoint
★ 4.1k • written 29 days ago by
nhaus
▴ 30
0
votes
7
replies
426
views
DESeq filtering specific to contrasts
DESeq2
updated 19 days ago by
Michael Love
42k • written 11 weeks ago by
Emma
• 0
2
votes
6
replies
410
views
How to make boxplot with pairwise and overall p value from Limma
t.test
boxplot
limma
anova
4 weeks ago • updated 29 days ago
Chris
• 0
2
votes
9
replies
397
views
Batch/method effect correction on a cohort of patients with RUVseq and DESeq2
DESeq2
RUVSeq
RUVseq
BatchEffect
DES
updated 12 days ago by
James W. MacDonald
65k • written 13 days ago by
Alexandre
• 0
2
votes
2
replies
392
views
[Diffbind] Blacklist error: Error: Invalid Normalization and Unable to apply Blacklist/Greylist
DiffBind
Normalization
26 days ago
Henry
▴ 10
0
votes
8
replies
356
views
DESeq2 output used for PCA plot on R studio
DESeq2
RSTUDIO
PCAtools
updated 13 days ago by
swbarnes2
★ 1.3k • written 15 days ago by
Aaliya
• 0
1
vote
3
replies
352
views
Why is my gene not significantly differentially expressed?
limmaGUI
edgeR
limma
updated 23 days ago by
Gordon Smyth
50k • written 28 days ago by
Simran
• 0
0
votes
1
reply
346
views
Gene names from Matrix
BioCor
StarBioTrek
MatrixGenerics
updated 28 days ago by
Lluís Revilla Sancho
▴ 730 • written 7 months ago by
karmasstark
• 0
0
votes
6
replies
343
views
unable to access Annotationhub latest record
DNAMethylation
AnnotationHub
EPICv2manifest
annotation
updated 27 days ago by
Tim Peters
▴ 180 • written 5 weeks ago by
s.malik
• 0
3
votes
3
replies
343
views
permutation test in edgeR
rna-seq
edgeR
updated 17 days ago by
Gordon Smyth
50k • written 4 weeks ago by
Netanel
• 0
1
vote
2
replies
342
views
limma Intercept vs No-intercept models completely changing DMR results?
limma
DMRcate
MethylationArray
EPICV2
updated 4 days ago by
Gordon Smyth
50k • written 4 days ago by
Kim
• 0
1
vote
4
replies
339
views
Opposite sign of LFC in count plots of DEGs (DESeq2)
DESeq2
14 days ago • updated 12 days ago
winwater0928
• 0
1
vote
7
replies
333
views
Math expression in shapeCustom legend in EnhancedVolcano
EnhancedVolcano
13 days ago • updated 12 days ago
pl23
• 0
2
votes
3
replies
329
views
ComplexHeatmap: add pch to rowAnnotation when the input is a data frame with variable number of continuous columns
ComplexHeatmap
23 days ago • updated 22 days ago
daniel.carbajo
• 0
0
votes
7
replies
325
views
Methylation Analysis experiment design using Limma
DNAMethylation
limma
methylationArrayAnalysis
21 days ago • updated 15 days ago
hortense96
• 0
3
votes
1
reply
316
views
Use of negative binomial model for exponential decay rate estimation using DESeq (or similar)
DESeq2
updated 14 days ago by
Michael Love
42k • written 14 days ago by
i.sudbery
▴ 40
1
vote
2
replies
313
views
CombineArrays for EPIC and EPIC V2
EPICV1
Methylation
combineArrays
minifi
EPICv2manifest
4 weeks ago • updated 4 days ago
Kim
• 0
0
votes
5
replies
304
views
Error for AnnotationForge makeOrgPackageFromNCBI function
OrgDb
AnnotationForge
updated 14 days ago by
James W. MacDonald
65k • written 16 days ago by
Gayatri
• 0
1
vote
0
replies
293
views
Tutorial:
Installing/switching between versions of R/Rstudio/Bioconductor on personal machine (Linux | Ubuntu)
bioconductor
Install
19 days ago
BioinfGuru
▴ 30
3
votes
4
replies
281
views
Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it does not happen in Linux
GenomAutomorphism
updated 6 hours ago by
Mike Smith
★ 6.5k • written 2 days ago by
robersy
• 0
3
votes
6
replies
281
views
Surrogate variable analysis for paired RNA seq experiment
RUVSeq
sva
27 days ago
nhaus
▴ 30
2
votes
5
replies
276
views
get BM error
ensembldb
updated 14 days ago by
James W. MacDonald
65k • written 14 days ago by
Fara
• 0
0
votes
4
replies
272
views
DEG with low counts
DESeq2
updated 19 days ago by
Michael Love
42k • written 23 days ago by
Rajat
• 0
0
votes
3
replies
263
views
A ComBat-seq issue: the covariates are confounded
ComBat-seq
sva
updated 20 days ago by
W. Evan Johnson
▴ 850 • written 26 days ago by
schlumbohm
• 0
0
votes
3
replies
260
views
log2FoldChange value is way too different when compared with counts(dds)
DESeq2
updated 6 days ago by
Michael Love
42k • written 7 days ago by
HAK
• 0
0
votes
2
replies
259
views
Odd behavior of Rsubread::featureCounts when setting `nonOverlap = 0`
featureCounts
Rsubread
5 weeks ago • updated 19 days ago
isaac.vock
• 0
0
votes
2
replies
252
views
featureCounts reports an error:"featureCounts: input-files.c:2890: SAM_pairer_get_next_read_BIN: Assertion `l_name < 256' failed. Aborted (core dumpe…
metaMSdata
15 days ago • updated 13 days ago
shuwangxinze1996
• 0
0
votes
4
replies
250
views
Minfi - estimatecellscount
min
minfi
updated 9 days ago by
James W. MacDonald
65k • written 5 weeks ago by
clacarion
• 0
0
votes
2
replies
249
views
Regressing out batch effects
DESeq2
updated 28 days ago by
Michael Love
42k • written 4 weeks ago by
kys91240
• 0
2
votes
5
replies
248
views
How to retrieve gene ontology GO class from gene list?
GO.db
goTools
updated 13 days ago by
James W. MacDonald
65k • written 14 days ago by
bandconductor
• 0
0
votes
3
replies
247
views
Assist me in determining whether the analysis process using the limma package has been executed correctly.
GEOquery
ArrayExpress
limma
DifferentialExpression
updated 8 days ago by
James W. MacDonald
65k • written 9 days ago by
SSSJec
• 0
1
vote
2
replies
245
views
How to retrieve detailed regression results from deseq2
DESeq2
27 days ago • updated 18 days ago
Weiqian
▴ 10
0
votes
4
replies
242
views
Differential peaks that were not identified in diffbind were identified in diseq2
DiffBind
DESeq2
ATACSeq
21 days ago • updated 18 days ago
lonn
• 0
1
vote
2
replies
241
views
Get genomic coordinates of CpGs sites (chromosomes, genomic position)
GenomeWideAssociation
SequenceAnnotation
MethylationArray
GenomicDistributionsData
7 days ago • updated 6 days ago
Yveto
• 0
1
vote
4
replies
241
views
Error EPICv2 ChAMP
ChAMPdata
IlluminaHumanMethylationEPICv2anno.20a1.hg38
ChAMP
updated 16 days ago by
James W. MacDonald
65k • written 20 days ago by
Mirella Aurora
• 0
3
votes
3
replies
241
views
DESeq2: Contrast for two conditions combined versus one condition (A+B_vs_C)
DESeq2
contrasts
updated 9 days ago by
ATpoint
★ 4.1k • written 9 days ago by
bioinf
▴ 10
1
vote
3
replies
235
views
Getting Error in hclust(d, method = method): NA/NaN/Inf in foreign function call when trying to subset pheatmap
DESeq2
updated 10 hours ago by
ATpoint
★ 4.1k • written 6 days ago by
angelathuynh5
• 0
1
vote
0
replies
234
views
News:
Nominations for Bioconductor Awards 2024!
Recognition
Awards
Bioconductor
Bioc204
6 weeks ago • updated 1 day ago
shepherl
3.9k
0
votes
1
reply
234
views
How do I select a GC file (gc.wig)? (How to determine the inputdata of HMMcopy)
CopyNumberPlots
HMMcopy
CopyNumberVariation
updated 7 days ago by
James W. MacDonald
65k • written 8 days ago by
SSSJec
• 0
3
votes
3
replies
231
views
DESeq2 - dispersion estimation when there is none
DESeq2
updated 15 days ago by
Michael Love
42k • written 16 days ago by
WardDeb
• 0
0
votes
4
replies
230
views
Storing directories inside an ExperimentHub package?
ExperimentHub
28 days ago • updated 26 days ago
Tim Barry
• 0
0
votes
6
replies
228
views
Once again a "Model matrix not full rank"
DESeq2
design
coldata
updated 4 days ago by
swbarnes2
★ 1.3k • written 5 days ago by
arfranco
▴ 130
1
vote
3
replies
227
views
Deseq2. decontXcounts not integers. Useful alternative?
DESeq2
celda
updated 12 days ago by
Michael Love
42k • written 12 days ago by
JalapenoCornbread
• 0
0
votes
2
replies
223
views
Interaction term vs grouping variable design considerations
DESeq2
DifferentialExpression
updated 28 days ago by
Michael Love
42k • written 4 weeks ago by
nhaus
▴ 30
3
votes
4
replies
223
views
Help with error using camera() in limma
CAMERA
limma
8 days ago • updated 7 days ago
Chris
• 0
1
vote
2
replies
221
views
significant difference of p-values with dream() after updating VariancePartition
variancePartition
20 days ago • updated 19 days ago
Le
• 0
3
votes
2
replies
219
views
Cannabis and KEGGgraph
KEGGREST
GenomicDistributionsData
KEGGgraph
KEGG
updated 16 days ago by
Guido Hooiveld
★ 3.9k • written 18 days ago by
fernanda.backsouza
▴ 10
1
vote
3
replies
217
views
Different Shrunk Log2FC according with reference
DESeq2
updated 14 days ago by
Michael Love
42k • written 14 days ago by
andrebolerbarros
▴ 20
3
votes
3
replies
215
views
Reading huge bismark coverage files using bbseq::read.bismark
HDF5Array
BiocGenerics
BiocParallel
Biobase
26 days ago • updated 20 days ago
jacques.imbert
• 0
201 results • Page
2 of 5
Recent ...
Replies
Comment: deseq2 results
by
swbarnes2
★ 1.3k
Cross-posted https://www.biostars.org/p/9594331/#9594458
Answer: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
by
Mike Smith
★ 6.5k
The other answers here are all good and demonstrate the issue with floating point comparisons nicely. I'd like to add that your particular…
Comment: More regions in union when increasing DBA$config$mergeOverlap
by
Ian D.
▴ 70
Nudging my question. I could do with confirmation before proceeding with my current projects. Thank you.
Comment: Extremely small p-values using Limma for proteomic data
by
Robert Castelo
★ 3.3k
From the point of view of visualization, I find handy the fact that raw p-values give more resolution than adjusted ones, which allows one …
Comment: deseq2 results
by
ATpoint
★ 4.1k
> the results are not satisfactory to me Well, what is your question? The DESeq2 vignette covers how to do analysis, and some diagnostics…
Votes
A: Volcanoplot with limma - RAW P-values or Adj.P-Values
Answer: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
Comment: Mac ARM64 build report for BioC 3.19 from 'kjohnson3' reporting ERROR which it
Answer: minfi latest update for EPIC V2 chip
A: Error in DESeqDataSet : some values in assay are not integers
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