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Comment: Joining different datasets and analyzing using the group-specific condition effe
by
James W. MacDonald
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There is an example of how you can do this in the [DESeq vignette][1].
An alternative would be to fit a linear mixed model using the `vari…
Comment: Joining different datasets and analyzing using the group-specific condition effe
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jason.chai
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@mikelove Could I please ask if a similar method would work my set of samples?
I have 255 samples which have been generated from 76 subject…
Comment: Clarification on counting in Rsubread (featureCounts)
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Luca
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Thanks to both of you for your helpful suggestions.
The counting was already strand-specific at both the exon and gene levels. I resolved …
Comment: Clarification on counting in Rsubread (featureCounts)
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Gordon Smyth
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Have you told featureCounts to do strand-specific counting?
As pointed out by Frances Turner, you can lose reads if they overlap the gene …
Answer: Clarification on counting in Rsubread (featureCounts)
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Frances Turner
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You should look at the summary files produced by feature counts. This gives a breakdown of what happened to reads that were not assigned. Y…
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