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TCGA
•
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0
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547
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One error problem occurred caused by data type during using GDCprepare function of TCGAbiolinks to handle TCGAbiolinks BRCA data
GDCRNATools
TCGA
11 months ago • updated 10 months ago
glaciya2018
• 0
9
votes
18
replies
3.9k
views
Generation of QSEA TCGA 450K Human Methylation Calibration Dataset
illimina 450k methylation
qsea
MEDIPS
TCGA
updated 14 months ago by
Matthias Lienhard
▴ 280 • written 8.0 years ago by
justinburgener
▴ 20
0
votes
2
replies
1.0k
views
TCGA counts to TPM from recount3 do not seem to right
recount3
TCGA
RNASeq
updated 16 months ago by
Leonardo Collado Torres
★ 1.1k • written 17 months ago by
tangming2005
▴ 200
0
votes
1
reply
824
views
GenomicDataCommons: Retrieve patient data for RPPA samples
TCGA
GenomicDataCommons
GDC
written 21 months ago by
António Miguel de Jesus Domingues
▴ 510
1
vote
1
reply
766
views
WGCNA Cluster batch effect
BatchEffect
TCGA
WGCNA
updated 21 months ago by
ATpoint
★ 4.7k • written 21 months ago by
khaserdene99
• 0
1
vote
3
replies
1.6k
views
Can i perform DEseq2 from 2 different rse objects download from RECOUNT database
DESeq2
recount
GTEx
TCGA
recount3
updated 22 months ago by
ATpoint
★ 4.7k • written 22 months ago by
David
▴ 20
0
votes
1
reply
941
views
TCGA RNAseq Normalization
normalization
RNASEQ
Normalization
TCGAutils
TCGA
updated 2.3 years ago by
ATpoint
★ 4.7k • written 2.3 years ago by
Deniz
• 0
2
votes
9
replies
3.4k
views
Stuck on how to voom-limma on RSEM data from TCGA
TCGA
r
RNASeqData
voom
limma
2.8 years ago • updated 2.7 years ago
Peipei
• 0
2
votes
2
replies
2.7k
views
Create TPM gene tables/dataframe from ReCount3
recount3
TCGA
GTEx
DESeq2
2.8 years ago
David
▴ 20
3
votes
2
replies
2.7k
views
Difference in TCGA RNAseq expression values between GDC harmonized and legacy data
GDC
TCGA
tcgabiolinks
updated 2.9 years ago by
cute scientist
• 0 • written 6.3 years ago by
Antonio Ahn
▴ 10
4
votes
5
replies
2.0k
views
GDCprepare() can never complete request ("Error: cannot allocate vector")
TCGAbiolinks
TCGA
updated 2.9 years ago by
biologsr
• 0 • written 4.5 years ago by
aush
▴ 40
1
vote
1
reply
1.7k
views
recount3 - download bigwig files for TCGA data
recount3
recount
TCGA
updated 3.0 years ago by
Leonardo Collado Torres
★ 1.1k • written 3.0 years ago by
Xuebing
▴ 10
0
votes
2
replies
1.2k
views
Removing paired samples for DESeq2 analysis
RNASeq
DifferentialExpression
DESeq2
tcga
3.2 years ago
IamFrofro
• 0
3
votes
2
replies
1.5k
views
How to obtain clinical data from TCGA via Bioconductor GenomicDataCommons
TCGA
GenomicDataCommons
updated 3.3 years ago by
Robert Castelo
★ 3.4k • written 3.3 years ago by
Hashirama
▴ 10
5
votes
9
replies
4.0k
views
Error via running GDCquey from TCGAbiolinks package
TCGA
Genome Data Common portal
updated 3.8 years ago by
H. Z. Amini
▴ 10 • written 6.7 years ago by
modarzi
▴ 10
0
votes
0
replies
1.0k
views
Determine whether RNAseq data from TCGA is paired or single-end?
TCGA
TCGAbiolinks
3.9 years ago
rebeliscu
• 0
0
votes
1
reply
1.1k
views
Getting number of cases added by date to TCGA project with `GenomicDataCommons`
TCGA
GenomicDataCommons
4.0 years ago
Ezgi
▴ 60
0
votes
0
replies
1.2k
views
Job:
PhD position in Developmental Neurobiology and Bioinformatics
rnaseq
neurobiology
tcga
Job
4.4 years ago
Laurent Gatto
1.6k
0
votes
0
replies
981
views
Identify significantly different DNA methylation probes in tumor and normal samples GBM ELMER R package
ELMER
TCGA
GEO
4.5 years ago
andrade4beatriz
• 0
0
votes
5
replies
4.4k
views
Retrieve TCGA expression data for a single gene
TCGA
TCGAbiolinks
updated 4.6 years ago by
Tiago Chedraoui Silva
▴ 260 • written 4.6 years ago by
aush
▴ 40
4
votes
7
replies
5.2k
views
Analyzing TCGA RSEM Data with DESeq2 downloaded from FireBrowse
deseq2
tximport
tcga
rsem
firebrowse
updated 4.8 years ago by
Kevin Blighe
★ 4.0k • written 4.8 years ago by
J. Smith
• 0
0
votes
2
replies
1.9k
views
Recount2 Bigwigs for TCGA
R
TCGA
recount2
recount
updated 5.0 years ago by
Leonardo Collado Torres
★ 1.1k • written 5.0 years ago by
alex.v.nesta
• 0
4
votes
7
replies
1.5k
views
'NA' found under Gene Names in the list of top differentially expressed genes found using edgeR
edger
differential expression
TCGA
Gene ID
updated 5.0 years ago by
James W. MacDonald
68k • written 5.0 years ago by
fawazfebin
▴ 60
0
votes
0
replies
934
views
Problem for downloading TCGA cancer data by TCGAbiolinks
TCGAbiolinks
GDC
TCGA
5.3 years ago
modarzi
▴ 10
0
votes
4
replies
1.6k
views
problem for downloading TCGA data by TCGAbiolikns package
TCGAbiolinks
TCGA
GDC
5.3 years ago
modarzi
▴ 10
2
votes
3
replies
994
views
DESeq2 doesn't return the most drastically DEG
deseq2
tcga
5.4 years ago
ndjayne
• 0
2
votes
2
replies
3.3k
views
DESeq2: design = ~1 For Normalisation Only.
deseq2
limma
normalization
tcga
rnaseq
updated 5.4 years ago by
Michael Love
43k • written 5.4 years ago by
Alex Greenshields-Watson
• 0
1
vote
2
replies
1.5k
views
TCGAbiolinks : Bisulfite-seq data : Legacy archive
tcga
bisulfite
tcgabiolinks
updated 5.7 years ago by
Tiago Chedraoui Silva
▴ 260 • written 5.7 years ago by
DS
• 0
1
vote
1
reply
1.6k
views
TCGA isoform expression quantification file - how to reshape data
data.frame
tcga
reshape data
updated 5.8 years ago by
Steve Lianoglou
★ 13k • written 5.8 years ago by
mario.zanfardino
▴ 160
1
vote
2
replies
1.7k
views
TCGA MAF (Mutations) data
TCGA
MAF
GDC
snp
mutations
updated 5.9 years ago by
Sean Davis
21k • written 5.9 years ago by
mario.zanfardino
▴ 160
0
votes
1
reply
1.4k
views
How to download the TCGA SKCM subtype information?
TCGA
melanoma
TCGA
SKCM
5.9 years ago
syrttgump
▴ 20
5
votes
4
replies
2.3k
views
Perform correlation analysis between miRNA and mRNA gene expression data on the same TCGA dataset based on the curatedTCGAData
curatedTCGAData
MultiAssayExperiment
multiomics
TCGA
updated 6.0 years ago by
Levi Waldron
★ 1.1k • written 6.0 years ago by
svlachavas
▴ 840
0
votes
0
replies
1.3k
views
TCGAbiolinks specific mutation query
TCGAbiolinks
TCGA
Mutation
GDCquery
rnaseq
6.0 years ago
Yonatan Amzaleg
• 0
1
vote
3
replies
2.9k
views
Error via running GDCdownload() in TCGAbiolinks
TCGAbiolinks
TCGA
Download
updated 6.0 years ago by
Tiago Chedraoui Silva
▴ 260 • written 6.0 years ago by
modarzi
▴ 10
4
votes
6
replies
4.2k
views
DESeq2 vst normalization: Do I have a heteroscedasticity problem and if so, what can I do about it?
deseq2
normalization
rna-seq
TCGA
vst
updated 6.1 years ago by
Michael Love
43k • written 6.1 years ago by
Marc Saric
▴ 70
1
vote
5
replies
2.0k
views
Issue with TCGAbiolinks package.
TCGA
GDCprepare
6.1 years ago
salvocomplicazioni1
• 0
0
votes
0
replies
1.0k
views
Reference for minimum number of probes commonly used for segmentation of microarray data
segmentation
reference
CNA
TCGA
6.1 years ago
jlarsen728
• 0
4
votes
2
replies
1.4k
views
GenomicDataCommons request timeouts when cases() %>% ... %>% results_all()
genomicdatacommons
tcga
gdc
rest
http
updated 6.2 years ago by
Sean Davis
21k • written 6.2 years ago by
mk
▴ 20
0
votes
0
replies
1.3k
views
Tumor subtype classification when genes of gene set are missing from samples - GSVA approach
GSVA
geneset
RNASeq
TCGA
subtypes
6.2 years ago
Pietro
▴ 40
0
votes
0
replies
1.2k
views
GDC RNA seq files (replicates?)
gdc data
TCGA
gdcdownload
6.2 years ago
coyoung
▴ 10
2
votes
4
replies
1.7k
views
edgeR. Known cancer genes are not differentially expressed?
edger
tcga
updated 6.3 years ago by
Aaron Lun
★ 28k • written 6.3 years ago by
rina
▴ 30
0
votes
1
reply
1.3k
views
RNAseq gene expression analysis in Personalized genomics
TCGA
deseq2
edger
limma
updated 6.4 years ago by
Michael Love
43k • written 6.4 years ago by
bioc.vkas
• 0
2
votes
3
replies
1.8k
views
Problem for finding "Locus Link ID" from Annotation file
wgcna package
TCGA
LocusLinkID
genecode
ensembl
updated 6.4 years ago by
James W. MacDonald
68k • written 6.4 years ago by
modarzi
▴ 10
8
votes
12
replies
7.6k
views
MultiAssayExperiment: Error in dimnames(x) <- dn : length of 'dimnames' [2] not equal to array extent
multiassayexperiment
wide format
TCGA
updated 6.5 years ago by
Marcel Ramos
700 • written 6.5 years ago by
mario.zanfardino
▴ 160
8
votes
8
replies
8.2k
views
Voom transformation from counts and normalization to negative values
rnaseq
voom
limma
tcga
statistics
updated 6.5 years ago by
Ryan C. Thompson
★ 7.9k • written 6.5 years ago by
Beginner
▴ 60
1
vote
2
replies
1.5k
views
how to output and get the normalization gene expression data (similar to the FPKM gene expression data) from reads count data by DESeq2 and edgeR?
deseq2
edger
fpkm
counts
TCGA
updated 6.5 years ago by
Gordon Smyth
52k • written 6.5 years ago by
ZihaoXing
• 0
3
votes
4
replies
3.1k
views
TCGA biolinks - Samples with multiple profiles
tcgabiolinks
tcga
genomicdatacommon
6.6 years ago
gamma.jian
▴ 10
0
votes
5
replies
2.7k
views
DNA methylation analysis without raw data IDAT files
r
illimina 450k methylation
minfi
champ
tcga
updated 6.6 years ago by
Yuan Tian
▴ 290 • written 6.6 years ago by
Beginner
▴ 60
4
votes
2
replies
2.7k
views
Where to download expected counts instead of normalized RSEM for TCGA LUAD?
limma
tcga
updated 6.6 years ago by
Steve Lianoglou
★ 13k • written 6.6 years ago by
Ahdee
▴ 50
0
votes
2
replies
3.0k
views
Error: vector memory exhausted (limit reached?)
tcgabiolinks
tcga
cnv
mac os x
gaia
updated 6.6 years ago by
rreck
▴ 20 • written 6.6 years ago by
rina
▴ 30
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Comment: DiffBind custom normalization factor
by
Abbie
• 0
Hello! Thanks so much for adding dba.normalize and all the documentation in the manual! I'm hoping I can ask a follow-up question here. I…
Answer: CpGs with genomic positions greater than the chromosome size in EPICv2?
by
James W. MacDonald
68k
All of the annotation packages provided by Bioconductor are simply repackaging of existing data from one or more sources. In this case, the…
Comment: Using TPM from tximport of Salmon output for singscore
by
Kent
• 0
Thank you for the suggestions Michael. Would you say that after filtering out the low count genes, `rlog(counts, blind = T)` is doing the s…
Comment: Using TPM from tximport of Salmon output for singscore
by
Michael Love
43k
For clustering generally we recommend `vst()` see the workflow for example. For working with individual samples, I can see how a scaling/t…
Comment: DESeq2 model - How to test case/control time course model when taking account wi
by
Michael Love
43k
I'd recommend working with a local statistician or someone familiar with linear models in R. I have to reserve my time on the support site …
Votes
A: Machine learning in R book
Data preparation and Machine learning on time course RNAseq data
Data preparation and Machine learning on time course RNAseq data
Answer: Use of org.Rn.eg.db with GRCr8 reference genome
A: use tximport's countsFromAbundance when using DESeq2
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