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0
votes
2
replies
1.8k
views
Filter low counts OTUs from a MRfull table to prepare a heatmap
metagenomeseq
8.0 years ago
arguello.rguez
• 0
0
votes
1
reply
1.8k
views
How to draw venn diagram with four CSV files
R
SNP
venn diagram
updated 8.0 years ago by
James W. MacDonald
68k • written 8.0 years ago by
kwangseob
• 0
1
vote
3
replies
2.2k
views
RNA-seq Batch Correction
rna-seq
edger
limma
removebatcheffect()
updated 8.0 years ago by
Aaron Lun
★ 29k • written 8.0 years ago by
cvu
• 0
1
vote
3
replies
1.1k
views
Errors of loading HilbertVisGUI
R
8.0 years ago
xz2256
• 0
1
vote
1
reply
2.5k
views
How can I retrieve the gene names of my count matrix?
rnaseq
summarizeoverlaps
count matrix
updated 8.0 years ago by
James W. MacDonald
68k • written 8.0 years ago by
johnsonn573
• 0
0
votes
6
replies
1.9k
views
two factor chip with diffbind and edgeR (or DESeq2).
diffbind
edger
updated 8.0 years ago by
Aaron Lun
★ 29k • written 8.0 years ago by
james.dalgleish
▴ 30
0
votes
3
replies
1.4k
views
Is this procedure necessary in DESeq2 for my data
deseq2
multiple factor design
nested design
8.0 years ago
michael.steffen
▴ 20
0
votes
6
replies
8.1k
views
Error in DESeqDataSet(se, design = design, ignoreRank) : all variables in design formula must be columns in colData
deseq2
R
genomics
8.0 years ago
mokunf
• 0
3
votes
4
replies
3.5k
views
DESeq2 batch effect modeling and matrix not full rank
deseq2
rnaseq
rna-seq
updated 8.0 years ago by
Michael Love
43k • written 8.0 years ago by
emmak
▴ 20
1
vote
1
reply
1.3k
views
Custom regions in assignChromosomeRegion?
chippeakanno
updated 8.0 years ago by
Ou, Jianhong
★ 1.3k • written 8.0 years ago by
Roger
• 0
0
votes
2
replies
2.5k
views
merging gseaResult objects
clusterprofiler
gsea
updated 8.0 years ago by
Guangchuang Yu
★ 1.2k • written 8.0 years ago by
robmarin
• 0
0
votes
4
replies
2.4k
views
Gviz IdeogramTrack fails with Error: failed to load HTTP resource
gviz
gviz-package
rtracklayer
http
8.0 years ago
enricoferrero
▴ 680
2
votes
2
replies
1.1k
views
Moderated F-statistics for time course experiment
limma
rnaseq
updated 8.0 years ago by
Gordon Smyth
53k • written 8.0 years ago by
Perry Moerland
▴ 130
0
votes
0
replies
1.1k
views
How to get proteins which are associated with a specific pathway of interest and subset iRefIndex files which contain the relevant interactions
Path2PPI
8.0 years ago
moldach
▴ 20
0
votes
0
replies
1.4k
views
NOISeq: Need help with my data frame
R
noiseq
data.frame
8.0 years ago
aokoro
• 0
2
votes
4
replies
2.2k
views
error reading ballgown object
R
ballgown
8.0 years ago
l.j.leach
• 0
0
votes
0
replies
1.2k
views
Subset functionin R
R
ballgown
rnaseq
8.0 years ago
amy16
• 0
0
votes
3
replies
1.8k
views
What is the impact of using GOSeq to voom-adjusted RNA-seq Expressions ?
limma
limma-voom
goseq
updated 8.0 years ago by
Gordon Smyth
53k • written 8.0 years ago by
joelrosa
• 0
0
votes
1
reply
2.5k
views
ChAMP Methylation analysis error code "could not find function champ.load"
champ
methylationepic
updated 8.0 years ago by
Yuan Tian
▴ 290 • written 8.0 years ago by
csappleby-mallinder1
• 0
0
votes
0
replies
1.4k
views
Deconvolute bulk RNA-Seq from human epithelial tissue scrapings with immune/blood cell contamination
deconvolution
methods
8.0 years ago
casey.rimland
▴ 170
0
votes
2
replies
1.1k
views
Read Bam file using rbamtools::bamReader()
rbamtools
updated 8.0 years ago by
Martin Morgan
25k • written 8.0 years ago by
emadelhewihy
• 0
0
votes
0
replies
1.2k
views
Function to specify axis limits according to data range for node or parent node
ggcyto
autoplot
axis
8.0 years ago
anders.tondell
▴ 30
0
votes
1
reply
1.5k
views
[flowCore] linearization of logarithmic data seems to be calculated wrong
flowcore
flowcytometry
updated 8.0 years ago by
Jiang, Mike
★ 1.3k • written 8.0 years ago by
max.zhao
• 0
19
votes
7
replies
15k
views
DeSeq2 results converting ENSG IDs to Gene Symbols, more than one ENSG ID per Gene symbol
deseq2
ensembl
updated 8.0 years ago by
James W. MacDonald
68k • written 8.0 years ago by
casey.rimland
▴ 170
0
votes
5
replies
2.2k
views
Trouble using addMarkers from pRoloc on my own dataset
proloc
proteomics
msnset
8.0 years ago
moldach
▴ 20
0
votes
4
replies
1.3k
views
can DESeq2 be used to compare these miRNA read counts from 9 samples?
deseq2
rnaseq
updated 8.0 years ago by
Michael Love
43k • written 8.0 years ago by
susannehoward
• 0
3
votes
2
replies
1.4k
views
Prevent "New version" message to appear
package
updated 8.0 years ago by
Martin Morgan
25k • written 8.0 years ago by
nick
▴ 10
3
votes
6
replies
2.7k
views
gviz ideogramTrack connection error
gviz-package
gviz
updated 8.0 years ago by
Robert Ivanek
▴ 750 • written 8.0 years ago by
alperyilmaz
▴ 20
3
votes
6
replies
2.3k
views
Best way to design a contrast matrix for analysis of dataset
Limma
8.0 years ago
Nithisha
▴ 10
0
votes
0
replies
1.1k
views
Accessing the assembly data-output
R
rnaseq
ballgown
8.0 years ago
amy16
• 0
1
vote
1
reply
2.5k
views
Unexpected clustering of samples after batch effect removal with ComBat
ComBat
updated 8.0 years ago by
Gordon Smyth
53k • written 10.8 years ago by
jaro.slamecka
▴ 140
1
vote
9
replies
3.8k
views
Removing batch effects from microarray data based on only a subset of samples
limma
removebatcheffect()
ComBat
microarray
8.0 years ago
jaro.slamecka
▴ 140
1
vote
4
replies
2.0k
views
Using Human Protein Atlas annotations with pRoloc to interpretate subcellular location
pRoloc
annotation
subcellular location
updated 8.0 years ago by
Laurent Gatto
1.6k • written 8.0 years ago by
moldach
▴ 20
0
votes
0
replies
1.7k
views
Interpreting Affymetrix Human Genome U133 Plus 2.0 samples
gene expression
gene expression data
cel
affy
affymatrix
8.0 years ago
torgeirous
• 0
8
votes
3
replies
6.0k
views
DESeq2 with interaction using SVA
deseq2
sva
interaction term
grouping variable
deseq
8.0 years ago
kevin.kingsland
▴ 70
1
vote
1
reply
1.5k
views
Expression deconvolution using PSEA: applicable to RNA-seq?
deconvolution
rnaseq
psea
8.0 years ago
WouterDeCoster
▴ 130
6
votes
4
replies
6.3k
views
Are two biological replicates enough for a transcriptome analysis using DESeq2?
deseq2
updated 8.0 years ago by
Sean Davis
21k • written 8.0 years ago by
Jonas B.
▴ 40
3
votes
5
replies
2.9k
views
DEGs of microarray Data and hierarchical clustering and visualizing using heatmap
limma
heatmap
DEGs
updated 8.0 years ago by
Gordon Smyth
53k • written 8.0 years ago by
lawarde.ankita1
▴ 30
4
votes
6
replies
2.2k
views
DESeq2 design and stats question
deseq2
differential binding analysis
deseq
atac-seq
updated 8.0 years ago by
Michael Love
43k • written 8.0 years ago by
rbronste
▴ 60
0
votes
1
reply
982
views
ExpressionSet annotation with custom annotation file
annotation
microarray
R
8.0 years ago
genesignature
• 0
1
vote
7
replies
2.6k
views
Low Counts in top DE genes in DESeq2
deseq2
updated 8.0 years ago by
Michael Love
43k • written 8.0 years ago by
rrcutler
▴ 70
2
votes
0
replies
1.8k
views
News:
LAST CHANCE: May Institute 2018 - Computation and statistics for mass spectrometry and proteomics at Northeastern University in Boston
news
proteomics
course
training
msstats
News
8.0 years ago
mnchoi67
▴ 40
0
votes
0
replies
1.1k
views
Wtccc sanger dataset
SNP
8.0 years ago
hadeel.alzoubi
• 0
0
votes
4
replies
2.7k
views
rlog transformation producing outliers with very high log-transformed counts
deseq2
rlog
8.0 years ago
Frederik Ziebell
▴ 30
1
vote
3
replies
1.9k
views
biomaRT biotype TFs only
biomart
TF
biotype
ensembl
mus musculus
updated 8.0 years ago by
Mike Smith
★ 6.6k • written 8.0 years ago by
rbronste
▴ 60
4
votes
4
replies
9.4k
views
edgeR design matrix and contrasts: how to make contrast between groups that aren't shown in the design matrix columns?
edger
design and contrast matrix
contrast
limma contrast matrix
limma
updated 8.0 years ago by
Gordon Smyth
53k • written 8.0 years ago by
cafelumiere12
▴ 20
4
votes
4
replies
1.7k
views
what the first row without a gene name?
deseq2
updated 4.9 years ago by
James W. MacDonald
68k • written 8.0 years ago by
Raymond
▴ 20
1
vote
1
reply
2.7k
views
WGCNA - Choosing the best threshold
wgcna
sft
picksoftthreshold
threshold
rnaseq
updated 8.0 years ago by
Peter Langfelder
★ 3.0k • written 8.0 years ago by
Fabricio Almeida-Silva
▴ 40
0
votes
0
replies
1.5k
views
How to call "DBA_CONDITION" for mutiple conditions and How to control GLM program
cancer
deseq2
edger
CONDITIONS
DBA
8.0 years ago
arcucamila
• 0
0
votes
3
replies
2.1k
views
How to find multiple overlaps with pairwiseAlignment()?
biostrings
pairwisealignment
updated 8.0 years ago by
Hervé Pagès
16k • written 8.0 years ago by
kgorczak
▴ 10
46,717 results • Page
285 of 935
Recent ...
Replies
Comment: Joining different datasets and analyzing using the group-specific condition effe
by
James W. MacDonald
68k
There is an example of how you can do this in the [DESeq vignette][1]. An alternative would be to fit a linear mixed model using the `vari…
Comment: Joining different datasets and analyzing using the group-specific condition effe
by
jason.chai
• 0
@mikelove Could I please ask if a similar method would work my set of samples? I have 255 samples which have been generated from 76 subject…
Comment: Clarification on counting in Rsubread (featureCounts)
by
Luca
• 0
Thanks to both of you for your helpful suggestions. The counting was already strand-specific at both the exon and gene levels. I resolved …
Comment: Clarification on counting in Rsubread (featureCounts)
by
Gordon Smyth
53k
Have you told featureCounts to do strand-specific counting? As pointed out by Frances Turner, you can lose reads if they overlap the gene …
Answer: Clarification on counting in Rsubread (featureCounts)
by
Frances Turner
▴ 20
You should look at the summary files produced by feature counts. This gives a breakdown of what happened to reads that were not assigned. Y…
Votes
A: DESeq2: What is the unit of DESeq2 normalized read count (VST)? Is it tag per mi
Answer: Clarification on counting in Rsubread (featureCounts)
Comment: Clarification on counting in Rsubread (featureCounts)
C: ControlGenes/ housekeeping genes Deseq2
Answer: Clarification on counting in Rsubread (featureCounts)
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