Showing : Differential expression • reset
3
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4
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3.4k
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Protein differential Expression analysis
differential expression proteomics
updated 15 months ago by ★ 4.5k • written 6.9 years ago by • 0
0
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17
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4.0k
views
What causes this DESeq2 error at "fitting model and testing"?
deseq2 differential expression error
updated 17 months ago by • 0 • written 7.1 years ago by • 0
3
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3
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3.9k
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Using PEER to identify hidden confounders in RNA-seq data (not working)
PEER RNA-seq differential expression hidden confounders
updated 2.7 years ago by ▴ 30 • written 5.8 years ago by ▴ 40
4
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2
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1.8k
views
Moderated F-test followed by moderated t-test in limma
limma differential expression
updated 4.2 years ago by 51k • written 4.2 years ago by • 0
5
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3
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1.6k
views
Latent factors for differential expression
deseq2 zinbwave 10X scRNA-seq Differential expression
updated 4.3 years ago by 42k • written 4.3 years ago by ▴ 20
3
votes
1
reply
1.2k
views
Obtain p-values for group * condition interaction using DESeq2?
DESeq2 interaction differential expression
updated 4.4 years ago by 42k • written 4.4 years ago by ▴ 160
6
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5
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2.9k
views
edgeR + tximport: calcNormFactors and the usage of offsets
edgeR tximport differential expression
4.5 years ago hollandademello ▴ 20
1
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1
reply
1.9k
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Differential expression on Nanostring normalised protein data
R Differential expression
updated 4.5 years ago by ★ 4.0k • written 4.5 years ago by • 0
0
votes
4
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1.5k
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Agilent two-color microarray analysis with limma: 2x3 factorial design
limma microarray contrast matrix 2x3 factorial design differential expression
updated 4.6 years ago by 51k • written 4.6 years ago by • 0
2
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2
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838
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edgeR - Correct approach to compare abundances of genomic regions?
edger differential expression
updated 4.6 years ago by 51k • written 4.6 years ago by ▴ 20
0
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0
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844
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Problems with cummeRbund
plot transcriptomics differential expression cummeRbund
4.6 years ago raquelponti • 0
0
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1
reply
813
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using deseq2 for intronic counts for 2 conditins with replicates
deseq2 intron differential expression
updated 4.6 years ago by 42k • written 4.6 years ago by ▴ 40
4
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7
replies
1.4k
views
'NA' found under Gene Names in the list of top differentially expressed genes found using edgeR
edger differential expression TCGA Gene ID
updated 4.6 years ago by 67k • written 4.6 years ago by ▴ 60
7
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4
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5.4k
views
Limma when to use zero intercept
limma regression differential expression linear model
updated 4.7 years ago by 51k • written 4.7 years ago by • 0
2
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1
reply
718
views
Detecting the power in differential expression
POWER DESEQ2 edger LIMMA DIfferential expression
5.0 years ago Beginner • 0
2
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3
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1.6k
views
DESeq2 2x2 factorial design: find genes unique to factor A?
deseq2 deseq differential expression contrasts
updated 5.0 years ago by 42k • written 5.0 years ago by ▴ 180
0
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1
reply
959
views
How to handle batch effect and interaction with experimental variables
deseq2 statistics differential expression
5.0 years ago A • 0
0
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0
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1.7k
views
GDCprepare error in TCGAbiolinks
tcgabiolinks gdcprepare differential expression
5.1 years ago fawazfebin ▴ 60
9
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11
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2.9k
views
dream vignette says limma-voom repeated measures two-group comparison needs two duplicateCorrelation rounds which differs from limma user's guide and…
dream variancePartition limma differential expression repeated measures
updated 5.1 years ago by 51k • written 5.1 years ago by ▴ 20
5
votes
8
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2.0k
views
Unexpected results while analyzing a subset of genes in GEO RNAseq dataset containing TPM values
R limma TPM rnaseq differential expression
updated 5.1 years ago by ★ 13k • written 5.1 years ago by ▴ 30
3
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2
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2.2k
views
Variation between technical replicates (DESeq2, batch effects)
rnaseq deseq2 differential expression batch effect replicates
updated 5.2 years ago by 42k • written 5.2 years ago by ▴ 10
5
votes
6
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1.5k
views
is our edgeR code doing what we think it does?
edger differential expression rna-seq
updated 5.2 years ago by ▴ 870 • written 5.2 years ago by ▴ 10
0
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1
reply
2.0k
views
Minimum number of cells required for the MAST
MAST scRNAseq differential expression
5.2 years ago Andrew_McDavid ▴ 270
26
votes
17
replies
8.2k
views
DESeq2 1.20.0 lfcshrink function
deseq2 differential expression logfoldchange lfcshrink lfcthreshold
updated 3.5 years ago by 42k • written 6.4 years ago by ▴ 40
2
votes
4
replies
1.5k
views
Majority upregulated genes in edgeR
edger differential expression genes fold change
updated 5.4 years ago by ★ 28k • written 5.4 years ago by • 0
1
vote
1
reply
1.1k
views
All Pairwise Comparisons with DESeq2 While Controlling for Batch Effects: Does Reference Level Matter?
deseq2 differential expression batch correction design
updated 5.4 years ago by 42k • written 5.4 years ago by ▴ 40
4
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4
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1.6k
views
Batch Correction: DESeq2 Linear Model vs Batch-Correction-Specific Algorithms (RUV, ComBat, ect.)
deseq2 differential expression batch correction
updated 5.4 years ago by 42k • written 5.4 years ago by ▴ 40
1
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12
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1.6k
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RNA-Seq and edgeR: remarkable differences between cpms and fitted values
RNA-Seq edger fitted values differential expression normalization
updated 5.4 years ago by 51k • written 5.4 years ago by ▴ 90
4
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5
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4.4k
views
How does limma calculate the log2fc?
Limma Differential Expression Time-course Analysis
updated 5.5 years ago by 51k • written 5.5 years ago by ▴ 10
1
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1
reply
1.1k
views
How to normalize RNA-seq when 50% genes are DE? (TMM)
limma TMM differential expression normalization rnaseq
updated 5.6 years ago by 67k • written 5.6 years ago by • 0
0
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1
reply
1.2k
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Scran: Performing DE after cell-typle identifaction on the corrected and integrated datasets
Scran MNN_correct Differential expression single-cell
5.7 years ago baldissera152 • 0
5
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1
reply
1.4k
views
Limma - Using removeBatchEffect before differential expression analysis
limma batch effect differential expression
updated 5.7 years ago by 67k • written 5.7 years ago by • 0
0
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3
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1.7k
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How to handle replicates and treatment conditions in TissueEnrich package
rna-seq TissueEnrich tissue-specific expression differential expression
updated 5.8 years ago by ▴ 10 • written 5.8 years ago by • 0
1
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1
reply
1.5k
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PLAGE method fails in GSVA if an overlap between gene sets and genes in expressions is less than two
gsva plage differential expression gene set enrichment
5.9 years ago krassowski.michal ▴ 40
2
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7
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2.5k
views
Get the countsFromAbundance as limma input
tximport limma deseq2 differential expression
updated 6.2 years ago by 51k • written 6.2 years ago by • 0
15
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12
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2.8k
views
ZINB-WaVE + DESeq2 misses low-expressed, single-condition genes that look like they should be markers
deseq2 ZINB-WaVE single cell RNA-seq differential expression
updated 6.3 years ago by 42k • written 6.3 years ago by ▴ 60
2
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3
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1.7k
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Global expression shift using RUVSeq
ruvseq rnaseq normalization differential expression
updated 6.4 years ago by ▴ 980 • written 6.4 years ago by • 0
1
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4
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1.7k
views
venDiagram after limma showing more genes after specifying up or down regulated specifically
venn diagram venndiagram limma microarray differential expression
updated 6.4 years ago by 67k • written 6.4 years ago by ▴ 40
5
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1
reply
1.2k
views
DE analysis when large portion of transcriptome is changed
rna-seq edger ebseq differential expression
updated 6.4 years ago by ★ 28k • written 6.4 years ago by • 0
0
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4
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1.6k
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Deseq2 problem visualizing gene expression with batch corrected values
deseq2 limma differential expression visualization
updated 6.4 years ago by 42k • written 6.4 years ago by • 0
1
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4
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1.4k
views
DE analysis of orthologs with varying sequence lengths using RSEM+tximport+DEseq2: normalization for sequence length?
deseq2 sequence length normalization RSEM tximport differential expression
updated 6.4 years ago by 42k • written 6.4 years ago by ▴ 50
0
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0
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1.1k
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pair wise ballgown
ballgown differential expression
6.4 years ago zoe.ward • 0
0
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0
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1.4k
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Ballgown normalisation... why are still using FPKM?
rnaseq normalization differential gene expression differential expression
6.4 years ago manuelmendoza • 0
1
vote
6
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1.5k
views
differential expression analysis with big dataset and >500 surrogate variables
SVA deseq2 differential expression LRT
6.5 years ago aec ▴ 90
0
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1
reply
1.1k
views
Interpretation of p-value plot
p values differential expression limma
updated 6.5 years ago by 51k • written 6.5 years ago by • 0
5
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4
replies
1.4k
views
Finding genes respond differently to treatment between many genotype comparison
edgeR nested design differential expression
updated 6.6 years ago by 51k • written 6.6 years ago by • 0
0
votes
3
replies
1.0k
views
interaction model deseq2 specific DE genes
deseq2 interaction model differential expression
updated 6.6 years ago by 42k • written 6.6 years ago by ▴ 90
6
votes
4
replies
1.7k
views
Fitting Gamma GLMs for examining differential expression
deseq2 edger differential expression
updated 6.7 years ago by ★ 7.9k • written 6.7 years ago by ▴ 10
7
votes
10
replies
6.5k
views
Justification for collapsing technical replicates in DESeq2
deseq2 rnaseq replicate differential expression
updated 6.7 years ago by ▴ 30 • written 8.3 years ago by ▴ 50
7
votes
11
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2.9k
views
Analysis of pathways using edgeR
rnaseq differential expression edgeR kegg
updated 6.7 years ago by 51k • written 6.7 years ago by ▴ 60
50 results • Page 1 of 1
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Replies
Comment: [How to construct a model.matrix ] by kyj2226 • 0
Thanks to you, I learn a lot. Many thanks, Yujin.
Comment: How to extract phastcons score for all protein coding genes and lncRNAs using Ge by rania.alanany • 0
thank you for your advice, I posted separate post.
Comment: How to extract phastcons score for all protein coding genes and lncRNAs using Ge by Robert Castelo ★ 3.4k
Hi, this is a long comment thread on 4-year old post, please open a new post with you question. To increase the odds of getting useful answ…
Answer: DESeq2 rerun returns different results by ATpoint ★ 4.5k
DESeq2 is deterministic if you use same input, code and software versions. Make sure to version-control your environment by at least tracki…
Answer: [How to construct a model.matrix ] by Gordon Smyth 51k
> Initially, I simply used > `lm(Epigenetic_age ~ Age_group + Age + Sex + PC1 + PC2 + PC3 + PC4 + PC5 + B + CD4T + CD8T + Mono + NK + Neutr…
Votes
Answer: [How to construct a model.matrix ]
C: DESeq2 baseMean values for each sample
C: DESeq2 baseMean values for each sample
Comment: ColData and ERROR: rows did not converge in beta, labelled in mcols(object)$beta
A: Some questions for remove batch effect by using mnnCorrect
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