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rna-seq
•
reset
0
votes
3
replies
1.4k
views
problem for finding soft power for constructing network in WGCNA algorithm
RNA-seq
WGCNA package
soft power
written 7.3 years ago by
modarzi
▴ 10
3
votes
4
replies
8.0k
views
Calculation of module membership in WGCNA
WGCNA
RNA-seq
ModuleMembership
Network
Hub gene
written 5.2 years ago by
Arindam
▴ 80
0
votes
1
reply
928
views
To count or not to count multi-overlapping reads?
RNA-Seq
featureCounts
Rsubread
updated 8 months ago by
Gordon Smyth
53k • written 8 months ago by
Arindam
▴ 80
5
votes
3
replies
29k
views
DESeq2: thousands of NA for pvalues?
deseq2
rna-seq
DEGs
cooks cutoff
written 6.8 years ago by
aishu.jp
▴ 30
1
vote
6
replies
1.6k
views
Volcano plot has many values around zero
RNA-seq
DESeq2
volcanoplot
updated 12 months ago by
Michael Love
43k • written 12 months ago by
nramakr
▴ 10
0
votes
2
replies
672
views
RNA-Seq p value range variation
DESeq2
pvalue
RNA-Seq
13 months ago
paul.meraner
• 0
0
votes
0
replies
582
views
WGCNA: Problem with selecting soft threshold
biologicalnetworkanalysis
genomes
RNA-Seq
co-expression
wgcna
13 months ago
Fernanda
• 0
1
vote
3
replies
1.0k
views
Including infectivity information to RNA-Seq analysis
RNASeqData
DESeq2
RNA-Seq
RNASeq
updated 17 months ago by
James W. MacDonald
68k • written 17 months ago by
Ben
▴ 50
2
votes
4
replies
1.1k
views
DESeq2- Mysterious interaction suppresses DEGs
DESeq2
PCAplot
RNA-Seq
DesignModel
updated 17 months ago by
Michael Love
43k • written 17 months ago by
shaunpeterson
• 0
1
vote
2
replies
1.0k
views
Multi-factorial, longitudinal disease progression analysis with unbalanced patient sampling - duplicateCorrelation or blocking?
rna-seq
duplicateCorrelation
limma
voom
18 months ago
Dylan.Sheerin
• 0
2
votes
6
replies
2.2k
views
Too many significant genes when integrating gtex and tcga
RNA-seq
DESeq2
updated 8 months ago by
Jane
• 0 • written 18 months ago by
Reza
• 0
3
votes
3
replies
1.8k
views
permutation test in edgeR
rna-seq
edgeR
updated 18 months ago by
Gordon Smyth
53k • written 19 months ago by
Netanel
• 0
0
votes
1
reply
1.4k
views
Human genes correlation to Bacterial genes
correlation
pearsoncorrelation
rna-seq
differential gene expression
updated 23 months ago by
ATpoint
★ 5.0k • written 7.6 years ago by
saad_k6
• 0
0
votes
2
replies
784
views
WGCNA less samples
RNA-Seq
WGCNA
Worms
23 months ago
davca
• 0
0
votes
4
replies
1.5k
views
DESeq2 : standardising variation between treatments
RNA-seq
DESeq2
2.1 years ago
ar14g12
• 0
0
votes
2
replies
4.9k
views
Dealing with multi-overlapping and multi-mapping reads
RNA-seq
featureCounts
ht-seq
written 5.3 years ago by
Arindam
▴ 80
2
votes
3
replies
4.8k
views
TPM from STAR mapped counts
GeneExpression
GenomicFeatures
RNA-Seq
TPM
updated 2.1 years ago by
Gordon Smyth
53k • written 2.1 years ago by
gingergeiger22
• 0
0
votes
3
replies
1.3k
views
RNAseq (Deseq2 and Dexseq2) analysis on two time points with expected same effect
DEXSeq
RNA-seq
DESeq2
updated 2.2 years ago by
Alejandro Reyes
★ 1.9k • written 2.2 years ago by
Alice
• 0
3
votes
4
replies
2.0k
views
lfcShrink() and batch effects in DESeq2
BatchEffect
lfcshrink
RNA-seq
DESeq2
2.2 years ago
Eva
▴ 10
0
votes
3
replies
1.2k
views
Fishpond with unbalanced dataset
rna-seq
salmon
fishpond
swish
updated 2.4 years ago by
Michael Love
43k • written 2.4 years ago by
samuelcjx
• 0
5
votes
7
replies
2.2k
views
Question about setting threshold using filtering by expression on genes in edgeR
filtering
RNA-Seq
CPM
edgeR
filterByExpr
updated 2.4 years ago by
Gordon Smyth
53k • written 2.5 years ago by
mohammedtoufiq91
▴ 10
0
votes
3
replies
1.3k
views
Is the gene-specific PCR efficiency a serious concern for intrasample comparisons in RNA-Seq?
RNA-Seq
normalization
PCR_efficiency
bias
intrasample_comparison
updated 2.5 years ago by
James W. MacDonald
68k • written 2.5 years ago by
FedeXandeR
• 0
0
votes
3
replies
1.5k
views
Why is RNA-Seq library size not constant among samples?
RNA-Seq
sequencer
library_size
sequency_depth
multiplexing
offtopic
updated 2.5 years ago by
James W. MacDonald
68k • written 2.5 years ago by
FedeXandeR
• 0
1
vote
1
reply
3.2k
views
FPKM t-test vs DEseq2
DESeq2
statistics
rna-seq
updated 2.6 years ago by
ATpoint
★ 5.0k • written 2.6 years ago by
SH
▴ 10
0
votes
1
reply
1.5k
views
Access modalities in h5 files to extract expression and atac data from 10xh5
rna-seq
Read10X_h5
atac
modalities
updated 2.6 years ago by
James W. MacDonald
68k • written 2.6 years ago by
Cynthia
• 0
2
votes
2
replies
1.3k
views
Multifactorial Design for DESEQ2
DESeq2
rna-seq
updated 2.6 years ago by
ATpoint
★ 5.0k • written 2.6 years ago by
genome_nerd
• 0
0
votes
1
reply
1.1k
views
Job:
Reopened: 'Omics data analyst for HPCBio, University of Illinois
SingleCell
Spatial
RNA-seq
2.7 years ago
Jenny Drnevich
★ 2.0k
0
votes
1
reply
1.3k
views
Error in DESeqDataSetFromHTSeqCount
DESeq2
RNA-seq
HTseq
DESeqDataSetFromHTSeqCount
updated 2.9 years ago by
Michael Love
43k • written 3.0 years ago by
abhay.kanodia
• 0
5
votes
9
replies
3.0k
views
GSVA perfomed on RSEM Expected Count data
GSVA
RNA-Seq
RSEM
Expected_Count
3.3 years ago
Yang Shi
▴ 10
2
votes
2
replies
2.0k
views
How to judge DEG analysis of these samples could be done by using limma::voom
limma
Clustering
voom
RNA-Seq
3.3 years ago
Yang Shi
▴ 10
0
votes
0
replies
1.3k
views
Job:
Computational Biology Contractor - Chinook Therapeutics
Patient_stratification
Multi-omics
RNA-seq
Kidney_Disease
3.3 years ago
eolson
• 0
2
votes
2
replies
1.6k
views
How to deal with stronger effect by donor than treatment
MDS
RNA-Seq
updated 3.3 years ago by
Gordon Smyth
53k • written 3.3 years ago by
Pei
• 0
0
votes
2
replies
4.1k
views
Incorporating spike-ins to rna-seq analysis
Spike-in
RNASeqR
DESeq2
Normalization
rna-seq
updated 2.2 years ago by
manuel.fernandez
• 0 • written 3.6 years ago by
cicely.day
• 0
3
votes
3
replies
4.7k
views
Using PEER to identify hidden confounders in RNA-seq data (not working)
PEER
RNA-seq
differential expression
hidden confounders
updated 3.6 years ago by
ahadli.farid
▴ 30 • written 6.8 years ago by
rodrigo.duarte88
▴ 40
5
votes
5
replies
3.1k
views
First principal component variance stabilised RNA-seq data correlates with total read counts per sample / library concentration
RNA-seq
PCA
control
DESeq2
Quality
3.7 years ago • updated 3.5 years ago
9906201a
▴ 20
1
vote
5
replies
3.3k
views
featureCounts parameter queries - minOverlap, fracOverlap, fracOverlapFeature
subread
Rsubread
featureCounts
RNA-seq
3.7 years ago
Lucy
▴ 60
1
vote
3
replies
2.2k
views
Two Genotypes Four Treatments RNA-seq Analysis
salmon
rna-seq
swish
bootstrap
fishpond
updated 3.7 years ago by
Michael Love
43k • written 3.7 years ago by
samuelcjx
• 0
0
votes
5
replies
5.9k
views
How to remove batch effect from RNA-seq without count data?
RUVSeq
FPKM
removeBatchEffect
RNA-seq
sva
updated 3.8 years ago by
ATpoint
★ 5.0k • written 4.6 years ago by
Xiaojie Cheng
• 0
0
votes
1
reply
1.4k
views
First time Statistical Analysis of RNA-seq
edgeR
RNA-Seq
log2
foldchange
q_value
updated 3.8 years ago by
Gordon Smyth
53k • written 3.8 years ago by
johndavidgriner74
• 0
1
vote
1
reply
3.9k
views
create S-plots with ropls package
s-plot
ropls
opls-da
rna-seq
updated 4.2 years ago by
Nathalie
▴ 10 • written 5.4 years ago by
andreia
▴ 10
0
votes
5
replies
1.9k
views
DTU analysis with tximport-DRIM-Seq-DEXSeq-stageR workflow starting from StrinTie output
RNA-seq
DRIMSeq
DEXSeq
rnaseqDTU
4.2 years ago
bioinformatic_trainee
▴ 10
0
votes
0
replies
1.4k
views
Job:
Senior Bioinformatician - University of Alabama at Birmingham
JobPosting
Transcriptomics
SingleCell
RNA-seq
4.3 years ago
lianov
• 0
0
votes
1
reply
1.5k
views
Interaction using a single factor model DESeq2
synergy
DESeq2
RNA-seq
contrast
4.3 years ago • updated 4.2 years ago
slcno
• 0
3
votes
5
replies
4.3k
views
Negative binomial fitting DESeq2
DESeq2
RNA-seq
updated 4.4 years ago by
Michael Love
43k • written 4.4 years ago by
Lucy
▴ 60
0
votes
1
reply
2.9k
views
Doing differential expression using FPKM , samples don't have replication
dge
fpkm
rna-seq
updated 4.4 years ago by
Kevin Blighe
★ 4.0k • written 4.4 years ago by
lkianmehr
• 0
2
votes
3
replies
1.8k
views
Differential expression error in edgeR - maybe from design matrix?
design
edgeR
matrix
RNA-seq
MDplot
updated 4.4 years ago by
Gordon Smyth
53k • written 4.4 years ago by
Alex
• 0
1
vote
1
reply
2.3k
views
Correlation analyses on TCGA data
RNA-seq
Normalization
edgeR
recount3
updated 4.4 years ago by
Leonardo Collado Torres
★ 1.1k • written 4.5 years ago by
mario.keller.1988
▴ 20
3
votes
4
replies
1.6k
views
need help regarding differential expression analysis
rna-seq
updated 4.5 years ago by
Kevin Blighe
★ 4.0k • written 4.5 years ago by
Rishav
▴ 20
0
votes
0
replies
1.4k
views
Job:
Computational Biologist, Blood Research Institute, Versiti, Wauwatosa, Wisconsin, USA
RNA-seq
Job
Single-Cell
4.5 years ago
rbrns
• 0
1
vote
4
replies
5.0k
views
NA values of count matrix in class DESeqDataSet
rna-seq
recount
updated 4.5 years ago by
Leonardo Collado Torres
★ 1.1k • written 8.1 years ago by
maleknias
• 0
418 results • Page
1 of 9
Recent ...
Replies
Comment: Failure to download resources (MeSHDb) from AnnotationHub
by
Steve Lianoglou
★ 13k
That fixed it, thanks Lori!
Comment: Suppress warning in RHDF5 write
by
nicolas.lhuillier
• 0
Thanks a lot for the quick and effective reply. I had tried "suppressWarnings" but not "suppressMessages".
Answer: Suppress warning in RHDF5 write
by
James W. MacDonald
68k
``` > fun <- function() message("this is a message") > fun() this is a message > suppressMessages(fun()) > ```
Answer: Failure to download resources (MeSHDb) from AnnotationHub
by
shepherl
4.2k
apologies. These are contributed by someone outside the core team. On upload I did not realize they included the older Bioconductor storageā¦
Answer: "Watched Tags" are being erased rather than updated
by
shepherl
4.2k
This should now be resolved
Votes
Answer: DESeq2 design for haplotype MPRA
Answer: Failure to download resources (MeSHDb) from AnnotationHub
Answer: Failure to download resources (MeSHDb) from AnnotationHub
Answer: Suppress warning in RHDF5 write
Answer: Suppress warning in RHDF5 write
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